Spectroscopy and Nuclear Magnetic Resonance Unit

Ramón Campos-Olivas Head of Unit
T +34 917328000 (Ext 3040)
rcampos@cnio.es

Technicians

Clara María Santiveri

This Unit focuses on the technical and scientific management of Nuclear Magnetic Resonance (NMR) Spectroscopy and molecular biophysics instrumentation available at the Structural Biology Programme. It provides CNIO researchers with equipment and experimental support for a variety of techniques used in biophysical studies of molecules involved in cancer. This includes the in vitro characterisation of the structure and dynamics of proteins by NMR, and characterisation of the affinity and kinetics of the interactions of proteins with other biopolymers and small molecules that could represent initial hits in the drug discovery process, or serve as research compounds for biophysical and functional studies. Furthermore, we use NMR to characterise the metabolic profiles of biofluids, cell growth media, and cell and tissue extracts from both animal models of cancer and human samples. In addition, in 2020, we adopted a mass spectrometer for the characterisation of intact proteins and for metabolite studies using HPLC-MS methods.

Publications

Izquierdo-Garcia JL, Comella-Del-Barrio P, Campos-Olivas R, Villar-Hernández R, Prat-Aymerich C, De Souza-Galvão ML, Jiménez-Fuentes MA, Ruiz-Manzano J, Stojanovic Z, González A, Serra-Vidal M, García-García E, Muriel-Moreno B, Millet JP, Molina-Pinargote I, Casas X, Santiago J, Sabriá F, Martos C, Herzmann C, Ruiz-Cabello J, Domínguez J (2020). Discovery and validation of an NMR-based metabolomic profile in urine as TB biomarker. Sci Rep 10, 22317. CNIO Publication.
Le Coq J, Camacho-Artacho M, Velázquez JV, Santiveri CM, Gallego LH, Campos-Olivas R, Dölker N, Lietha D (2017). Structural basis for interdomain communication in SHIP2 providing high phosphatase activity. Elife 6, e26640. CNIO Publication. Open Access
Bakiri L, Hamacher R, Graña O, Guío-Carrión A, Campos-Olivas R, Martinez L, Dienes HP, Thomsen MK, Hasenfuss SC, Wagner EF (2017). Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation. J Exp Med 214, 1387-1409. CNIO Publication.
San José-Enériz E, Agirre X, Rabal O, Vilas-Zornoza A, Sanchez-Arias JA, Miranda E, Ugarte A, Roa S, Paiva B, Estella-Hermoso de Mendoza A, Alvarez RM, Casares N, Segura V, Martín-Subero JI, Ogi FX, Soule P, Santiveri CM, Campos-Olivas R, Castellano G, de Barrena MGF, Rodriguez-Madoz JR, García-Barchino MJ, Lasarte JJ, Avila MA, Martinez-Climent JA, Oyarzabal J, Prosper F (2017). Discovery of first-in-class reversible dual small molecule inhibitors against G9a and DNMTs in hematological malignancies. Nat Commun 8, 15424. CNIO Publication. Open Access
Franco-Echevarría E, González-Polo N, Zorrilla S, Martínez-Lumbreras S, Santiveri CM, Campos-Olivas R, Sánchez M, Calvo O, González B, Pérez-Cañadillas JM (2017). The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition. Nucleic Acids Res 45, 10293-10305. CNIO Publication.
Burén S, Gomes AL, Teijeiro A, Fawal MA, Yilmaz M, Tummala KS, Perez M, Rodriguez-Justo M, Campos-Olivas R, Megías D, Djouder N (2016). Regulation of OGT by URI in Response to Glucose Confers c-MYC-Dependent Survival Mechanisms.. Cancer Cell 30, 290-307. CNIO Publication.
Fernández FJ, Ardá A, López-Estepa M, Aranda J, Peña-Soler E, Garces F, Round A, Campos-Olivas R, Bruix M, Coll M, Tuñón I, Jiménez-Barbero J, Vega MC (2016). Mechanism of sulfur transfer across protein-protein interfaces: The cysteine desulfurase model system.. ACS Catal 6, 3975-3984. CNIO Publication.
Assi HA, Harkness RW 5th, Martin-Pintado N, Wilds CJ, Campos-Olivas R, Mittermaier AK, González C, Damha MJ (2016). Stabilization of i-motif structures by 2′-ß-fluorination of DNA. Nucleic Acids Res 44, 4998-5009. CNIO Publication. Open Access
López-Guadamillas E, Fernández-Marcos PJ, Pantoja C, Muñoz-Martin M, Martínez D, Gómez-López G, Campos-Olivas R, Valverde AM, Serrano M (2016). p21Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARa. Sci Rep 6, 34542. CNIO Publication. Open Access
Morando MA, Saladino G, D'Amelio N, Pucheta-Martinez E, Lovera S, Lelli M, López-Méndez B, Marenchino M, Campos-Olivas R, Gervasio FL (2016). Conformational Selection and Induced Fit Mechanisms in the Binding of an Anticancer Drug to the c-Src Kinase. Sci Rep 6, 24439. CNIO Publication. Open Access
Bayó-Puxan N, Rodríguez-Mias R, Goldflam M, Kotev M, Ciudad S, Hipolito CJ, Varese M, Suga H, Campos-Olivas R, Barril X, Guallar V, Teixidó M, García J, Giralt E. (2016). Combined Use of Oligopeptides, Fragment Libraries, and Natural Compounds: A Comprehensive Approach To Sample the Druggability of Vascular Endothelial Growth Factor.. ChemMedChem 11, 928-939. CNIO Publication.
Barbero N, Marenchino M, Campos-Olivas R, Oliaro-Bosso S, Bonandini L, Boskovic J, Viscardi G, Visentin S (2016). Nanomaterials-protein interactions: the case of pristine and functionalized carbon nanotubes and porcine gastric mucin.. J Nanopart Res 18, 84. CNIO Publication.