This Unit focuses on the technical and scientific management of Nuclear Magnetic Resonance (NMR) Spectroscopy and molecular biophysics instrumentation available at the Structural Biology Programme. It provides CNIO researchers with equipment and experimental support on a variety of techniques for studying molecules involved in cancer. We work in close collaboration with groups from all Programmes to contribute our expertise and technologies to their research projects (see publications below for examples). They include the in vitro characterization of the structure and dynamics of proteins by NMR, the assessment of their homogeneity, molar mass and size by SEC-MALS and DLS, and the characterization of the conformational state and thermal stability by CD and nanoDSF. The Unit is also equipped with an ITC and two biosensor instruments (SPR and BLI) to study the affinity and kinetics of protein interactions with other biopolymers and with small molecules that could represent initial hits in the drug discovery process or research compounds for biophysical and functional studies. Furthermore, we use NMR to characterise the metabolic profiles of biofluids, cell growth media, and cell and tissue extracts from both animal models of cancer and human samples, and for chemical analytics. In addition, in 2021, we incorporated a multiple well microplate reader that includes numerous detectors (absorbance, fluorescence intensity, polarisation and time resolved modes, luminescence, alphascreen, etc.) with excellent sensitivity for both in-solution and adherent cells measurements, which has gained widespread use at CNIO.
Publications
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García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. (2022).Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. Mol Cell 82, 3438-3452. CNIO Publication.
Chaves-Arquero B, Martínez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jiménez MÁ, Calvo O, Pérez-Cañadillas JM. (2022).Structural basis of Nrd1-Nab3 heterodimerization. Life Sci Alliance 5, e202101252. CNIO Publication.
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