This Unit focuses on the technical and scientific management of Nuclear Magnetic Resonance (NMR) spectroscopy and molecular biophysics instrumentation available at the Structural Biology Programme. It provides CNIO researchers with equipment and experimental support for biophysical techniques in studies of molecules involved in cancer. This includes the in vitro characterisation of: i) the structure and dynamics of proteins by NMR and ii) the affinity and kinetics of protein interactions with other biopolymers and small molecules that could represent initial hits in drug discovery or research compounds for biophysical and functional studies. Furthermore, we use NMR to screen libraries of fluorinated fragments against macromolecular targets and to characterise the metabolic profiles of biofluids, cell growth media, and cell and tissue extracts from both animal models of cancer and human samples. The Unit is also endowed with a state-of-the-art, multiple well microplate reader equipped with diverse detectors (absorbance; fluorescence intensity and polarisation; time resolved fluorescence; luminescence and AlphaScreen) for in-solution and adherent cell measurements.
Publications
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Mellid S, García F, Leandro-García LJ, Díaz-Talavera A, Martínez-Montes ÁM, Gil E, Calsina B, Monteagudo M, Letón R, Roldán-Romero JM, Santos M, Lanillos J, Valdivia C, Martínez-Puente N, de Nicolás-Hernández J, Jiménez S, Pérez-Martínez M, Honrado E, Coloma J, Cerezo A, Santiveri CM, Esteller M, Campos-Olivas R, Caleiras E, Montero-Conde C, Rodríguez-Antona C, Muñoz J, Robledo M, Cascón A. (2023).DLST mutations in pheochromocytoma and paraganglioma cause proteome hyposuccinylation and metabolic remodeling. Cancer Commun 43, 838-843. CNIO Publication. Open Access
García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. (2022).Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. Mol Cell 82, 3438-3452. CNIO Publication.
Chaves-Arquero B, Martínez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jiménez MÁ, Calvo O, Pérez-Cañadillas JM. (2022).Structural basis of Nrd1-Nab3 heterodimerization. Life Sci Alliance 5, e202101252. CNIO Publication. Open Access
Open Access