This Unit focuses on the technical and scientific management of Nuclear Magnetic Resonance (NMR) Spectroscopy and molecular biophysics instrumentation available at the Structural Biology Programme. It provides CNIO researchers with equipment and experimental support for a variety of techniques used in biophysical studies of molecules involved in cancer. This includes the in vitro characterisation of the structure and dynamics of proteins by NMR, and of the affinity and kinetics of the interactions of proteins with other biopolymers and small molecules that could represent initial hits in the drug discovery process or research compounds for biophysical and functional studies. Furthermore, we use NMR to characterise the metabolic profiles of biofluids, cell growth media, and cell and tissue extracts from both animal models of cancer and human samples. In addition, in 2021, we successfully installed a multiple well microplate reader that includes numerous detectors (absorbance, fluorescence intensity, polarisation and time resolved modes, luminescence, alphascreen, etc.) with excellent sensitivity for both in-solution and adherent cells measurements.
- Clara María Santiveri
- (2022). Histone acetylation of bile acid transporter genes plays a critical role in cirrhosis. J Hepatol 76, 850-861. CNIO Publication.
- (2021). Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. Nat Commun 12, 3660. CNIO Publication. Open Access
- (2021). Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children.. Sci Rep 11, 12006-12017. CNIO Publication.
- (2020). Discovery and validation of an NMR-based metabolomic profile in urine as TB biomarker. Sci Rep 10, 22317. CNIO Publication.
- (2017). Discovery of first-in-class reversible dual small molecule inhibitors against G9a and DNMTs in hematological malignancies. Nat Commun 8, 15424. CNIO Publication. Open Access
- (2017). Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation. J Exp Med 214, 1387-1409. CNIO Publication.
- (2017). The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition. Nucleic Acids Res 45, 10293-10305. CNIO Publication.
- (2017). Structural basis for interdomain communication in SHIP2 providing high phosphatase activity. Elife 6, e26640. CNIO Publication. Open Access