Unidad de Espectroscopía y RMN

Ramón Campos-Olivas Jefe de Unidad
T +34 917328000 (Ext 3040)
rcampos@cnio.es

Técnicos de Laboratorio

Clara María Santiveri

This Unit focusses on the technical and scientific management of Nuclear Magnetic Resonance (NMR) spectroscopy and molecular biophysics instrumentation available at the Structural Biology Programme. It provides CNIO researchers with equipment and experimental support for biophysical techniques in studies of molecules involved in cancer. This includes: the in vitro characterisation of the structure and dynamics of proteins by NMR; and the characterisation of the affinity and kinetics of protein interactions with other biopolymers and small molecules of physiological relevance, or that could represent initial hits in drug discovery, and research compounds for biophysical and functional studies.

Furthermore, we use NMR to screen libraries of fluorinated fragments against macromolecular targets, and to characterise the metabolic profiles of biofluids, cell growth media, and cell and tissue extracts from both animal models of cancer and human samples. The Unit is also endowed with a state-of-the-art, multiple-well microplate reader equipped with diverse detectors (absorbance; fluorescence intensity and polarisation; time resolved fluorescence; luminescence and AlphaScreen) for in-solution and adherent cell measurements.

Publicaciones recientes

Rigual MDM, Angulo-Aguado M, Zagorac S, Álvarez-Díaz R, Benítez-Mondéjar M, Yi F, Martínez-Garay C, Santos-de-Frutos K, Kim E, Campos-Olivas R, Djouder N. (2025). Macrophages harness hepatocyte glutamate to boost liver regeneration. Nature 641, 1005-1016. Publicación CNIO.
Márquez-Moñino MÁ, Santiveri CM, de León P, Camero S, Campos-Olivas R, Jiménez MÁ, Sáiz M, González B, Pérez-Cañadillas JM (2025). The ALS drug riluzole binds to the C-terminal domain of SARS-CoV-2 nucleocapsid protein and has antiviral activity.. Structure 33, 39-50. Publicación CNIO.
Mellid S, García F, Leandro-García LJ, Díaz-Talavera A, Martínez-Montes ÁM, Gil E, Calsina B, Monteagudo M, Letón R, Roldán-Romero JM, Santos M, Lanillos J, Valdivia C, Martínez-Puente N, de Nicolás-Hernández J, Jiménez S, Pérez-Martínez M, Honrado E, Coloma J, Cerezo A, Santiveri CM, Esteller M, Campos-Olivas R, Caleiras E, Montero-Conde C, Rodríguez-Antona C, Muñoz J, Robledo M, Cascón A. (2023). DLST mutations in pheochromocytoma and paraganglioma cause proteome hyposuccinylation and metabolic remodeling. Cancer Commun 43, 838-843. Publicación CNIO. Open Access
Garrido A, Kim E, Teijeiro A, Sánchez PS, Gallo R, Nair A, Matamala Montoya M, Perna C, Vicent GP, Muñoz J, Campos-Olivas R, Melms JC, Izar B, Schwabe RF, Djouder N. (2022). Histone acetylation of bile acid transporter genes plays a critical role in cirrhosis. J Hepatol 76, 850-861. Publicación CNIO. Open Access
García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. (2022). Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. Mol Cell 82, 3438-3452. Publicación CNIO. Open Access
Sanz-Castillo B, Hurtado B, Vara-Ciruelos D, El Bakkali A, Hermida D, Salvador-Barbero B, Martínez-Alonso D, González-Martínez J, Santiveri C, Campos-Olivas R, Ximénez-Embún P, Muñoz J, Álvarez-Fernández M, Malumbres M (2022). The MASTL/PP2A cell cycle kinase-phosphatase module restrains PI3K-Akt activity in an mTORC1-dependent manner. EMBO J 42, e110833. Publicación CNIO. Open Access
Chaves-Arquero B, Martínez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jiménez MÁ, Calvo O, Pérez-Cañadillas JM. (2022). Structural basis of Nrd1-Nab3 heterodimerization. Life Sci Alliance 5, e202101252. Publicación CNIO. Open Access
Mulero F, Oteo M, Garaulet G, Magro N, Rebollo L, Medrano G, Santiveri C, Romero E, Sellek RE, Margolles Y, Campos-Olivas R, Arroyo AG, Fernández LA, Morcillo MA, Martínez-Torrecuadrada JL (2022). Development of anti-membrane type 1-matrix metalloproteinase nanobodies as immunoPET probes for triple negative breast cancer imaging. Front Med 24, 1058455. Publicación CNIO. Open Access
de la Calle Arregui C, Plata-Gómez AB, Deleyto-Seldas N, García F, Ortega-Molina A, Abril-Garrido J, Rodriguez E, Nemazanyy I, Tribouillard L, de Martino A, Caleiras E, Campos-Olivas R, Mulero F, Laplante M, Muñoz J, Pende M, Sabio G, Sabatini DM, Efeyan A (2021). Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. Nat Commun 12, 3660. Publicación CNIO. Open Access
Comella-Del-Barrio P, Izquierdo-Garcia JL, Gautier J, Doresca MJC, Campos-Olivas R, Santiveri CM, Muriel-Moreno B, Prat-Aymerich C, Abellana R, Pérez-Porcuna TM, Cuevas LE, Ruiz-Cabello J, Domínguez J (2021). Urine NMR-based TB metabolic fingerprinting for the diagnosis of TB in children.. Sci Rep 11, 12006-12017. Publicación CNIO.