Técnicos de Laboratorio
- Clara María Santiveri
The Unit unifies the technical and scientific management of Nuclear Magnetic Resonance (NMR) Spectroscopy and other molecular biophysics instrumentation available through the Structural Biology Programme. It provides CNIO researchers with equipment and technical support for a variety of techniques used in biophysical studies of molecules involved in cancer. This includes the application of NMR to the in vitro characterisation of the structure and dynamics of biomolecules (proteins in particular) and their interactions with other biopolymers, as well as with small molecules that could represent initial hits in the drug discovery process or research compounds for biophysical and functional studies. Furthermore, we use NMR to characterise the metabolic profiles of biofluids, cell growth media and cell and tissue extracts from both animal models of cancer and human samples.
Publicaciones
- (2017). Structural basis for interdomain communication in SHIP2 providing high phosphatase activity. Elife 6, e26640. Publicación CNIO.
Open Access - (2017). Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation. J Exp Med 214, 1387-1409. Publicación CNIO.
- (2017). Discovery of first-in-class reversible dual small molecule inhibitors against G9a and DNMTs in hematological malignancies. Nat Commun 8, 15424. Publicación CNIO. Open Access
- (2017). The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition. Nucleic Acids Res 45, 10293-10305. Publicación CNIO.
- (2016). Regulation of OGT by URI in Response to Glucose Confers c-MYC-Dependent Survival Mechanisms.. Cancer Cell 30, 290-307. Publicación CNIO.
- (2016). Mechanism of sulfur transfer across protein-protein interfaces: The cysteine desulfurase model system.. ACS Catal 6, 3975-3984. Publicación CNIO.
- (2016). Stabilization of i-motif structures by 2′-ß-fluorination of DNA. Nucleic Acids Res 44, 4998-5009. Publicación CNIO. Open Access
- (2016). p21Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARa.. Sci Rep 6, 34542. Publicación CNIO.
- (2016). Conformational Selection and Induced Fit Mechanisms in the Binding of an Anticancer Drug to the c-Src Kinase. Sci Rep 6, 24439. Publicación CNIO. Open Access
- (2016). Combined Use of Oligopeptides, Fragment Libraries, and Natural Compounds: A Comprehensive Approach To Sample the Druggability of Vascular Endothelial Growth Factor.. ChemMedChem 11, 928-939. Publicación CNIO.
- (2016). Nanomaterials-protein interactions: the case of pristine and functionalized carbon nanotubes and porcine gastric mucin.. J Nanopart Res 18, 84. Publicación CNIO.
- (2015). Alternative activation mechanisms of Protein Kinase B trigger distinct downstream signaling responses.. J Biol Chem 290, 24975-24985. Publicación CNIO.
- (2015). AMPK and PFKFB3 mediate glycolysis and survival in response to mitophagy during mitotic arrest.. Nat Cell Biol 17, 1304-1316. Publicación CNIO.
- (2015). MYC/PGC-1a Balance Determines the Metabolic Phenotype and Plasticity of Pancreatic Cancer Stem Cells.. Cell Metab 22, 590-605. Publicación CNIO.
- (2015). The N-terminal domain of MuB protein has striking structural similarity to DNA-binding domains and mediates MuB filament-filament interactions.. J Struct Biol 191, 100-111. Publicación CNIO.
- (2014). A switch from white to brown fat increases energy expenditure in cancer-associated cachexia.. Cell Metab 20, 433-447. Publicación CNIO.
- (2014). BuD, a helix–loop–helix DNA-binding domain for genome modification.. Acta Crystallogr D 70, 2042-2052. Publicación CNIO.
- (2014). Discovery of selective ligands for telomeric RNA G-quadruplexes (TERRA) through 19F-NMR based fragment screening.. ACS Chem Biol 97, 1559-1566. Publicación CNIO.