The Protein Crystallography Unit is a core facility that provides on-demand services at different levels, from the cloning, expression, and purification of high-quality proteins to the determination of their 3D structures, with the purpose to fulfil the demands of our users and to understand the function of their protein targets. Thus, we produce proteins for different types of assays and structural determination at low resolution by small-angle X-ray scattering (SAXS) or at atomic resolution by X-ray crystallography. The latter includes protein co-crystallisation in the presence of inhibitors or small fragments, a method that we routinely combine with the quantification of protein thermal stability (thermofluor assay) to aid the drug discovery process.
Publications
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Martin-Hurtado A, Contreras Contreras J, Sanchez-Wandelmer J, Zarzuela E, Muñoz IG, Muñoz J, Isasa M and Plaza-Menacho I (2025).The oncogenic CCDC6-RET fusion product is a dual ATP and ADP-dependent kinase that functions via cis-phosphorylation. BioRxiv (in press). CNIO Publication.
Cuesta N, Contreras MJ, Sanchez-Waldermer J, Soriano-Maldonado P, Martin-Hurtado A, Munoz IG, Llimargas M, Munoz J, Plaza- Menacho I (2023).An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-Src function. Nat Commun 14, 6548. CNIO Publication. Open Access
Lázaro-Gorines R, Pérez P, Heras-Murillo I, Adán-Barrientos I, Albericio G, Astorgano D, Flores S, Luczkowiak J, Labiod N, Harwood SL, Segura-Tudela A, Rubio-Pérez L, Nugraha Y, Shang X, Li Y, Alfonso C, Adipietro KA, Abeyawardhane DL, Navarro R, Compte M, Yu W, MacKerell AD Jr, Sanz L, Weber DJ, Blanco FJ, Esteban M, Pozharski E, Godoy-Ruiz R, Muñoz IG, Delgado R, Sancho D, García-Arriaza J, Álvarez-Vallina L. (2023).Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2.. Adv Sci 10, e2304818. CNIO Publication.
Open Access