The Protein Crystallography Unit is a core facility that provides specialised services focused on the structural investigation of biological systems at various levels of resolution and length scales. This is executed through a series of techniques, using synchrotron radiation, ranging from the identification of changes occurring in tissues, cells, and organelles (Fourier Transform Infrared spectroscopy, cryo-soft x-ray tomography, and phase-contrast X-ray imaging) to the characterisation of proteins and macromolecular complexes in-solution by SAXS (Small Angle X-ray Scattering), or at atomic resolution by X-ray crystallography.
Recent publications
- (2026). The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinase. Nat Commun (in press). CNIO Publication.
- (2026). Nanobodies as tools for studying human frataxin biology. Commun Biol (in press). CNIO Publication.
- (2025). Dissecting the molecular basis underlying mycobacterial cell-wall hydrolysis by the catalytic domains of D29LysA and DS6ALysA phage endolysins. Int J Biol Macromol 334, 148896. CNIO Publication.
- (2025). Redox-dependent activity and thioredoxin interaction of cyclophilin TgCyp21 from Toxoplasma gondii.. Int J Biol Macromol 330, 148019. CNIO Publication.
- (2025). Novel Inhibitors for MDM2-MDM4 E3 Ligase Potently Induce p53-Indepedent Apoptosis in Drug-Resistant Leukemic Cells. Molecules 30, 186. CNIO Publication.
- (2025). The oncogenic CCDC6-RET fusion product is a dual ATP and ADP-dependent kinase that functions via cis-phosphorylation. BioRxiv (in press). CNIO Publication.
- (2024). Small-molecule MMRi36 induces apoptosis in p53-mutant lymphomas by targeting MDM2/MDM4/XIAP for degradation. Front Oncol 14, 1462231. CNIO Publication.
- (2023). An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-Src function. Nat Commun 14, 6548. CNIO Publication.
Open Access - (2023). Recommendations for the classification of germline variants in the exonuclease domain of POLE and POLD1. Genome Med 15, 85. CNIO Publication.
- (2023). Molecular basis of the final step of cell division in Streptococcus pneumoniae. Cell Reports 42, 112756. CNIO Publication.
- (2023). Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2.. Adv Sci 10, e2304818. CNIO Publication.
- (2022). Small molecule MMRi62 targets MDM4 for degradation and induces leukemic cell apoptosis regardless of p53 status. Front Oncol 12, 933446. CNIO Publication.
- (2021). An Fc-free EGFR-specific 4-1BB-agonistic Trimerbody Displays Broad Antitumor Activity in Humanized Murine Cancer Models without Toxicity. Clin Cancer Res 27, 3167-3177. CNIO Publication.
- (2021). Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery. COMPUT STRUCT BIOTEC 19, 5392-5405. CNIO Publication.