Protein Crystallography Unit

Inés Muñoz Head of Unit
T +34 917328000 (Ext 3020)
imunoz@cnio.es

The Protein Crystallography Unit is a core facility that provides on-demand services at different levels, from the cloning, expression, and purification of proteins to the determination of their 3D structures, with the purpose to fulfil the demands of our users and to understand the function of their protein targets. Thus, we produce high-quality proteins for different types of assays and structural determination at low resolution by small-angle X-ray scattering (SAXS) or at atomic resolution by X-ray crystallography. The latter includes protein co-crystallisation in the presence of inhibitors or small fragments, a method that we routinely combine with the quantification of protein thermal stability (thermofluor assay) to aid the drug discovery process.

Publications

Cuesta N, Contreras MJ, Sanchez-Waldermer J, Soriano-Maldonado P, Martin-Hurtado A, Munoz IG, Llimargas M, Munoz J, Plaza- Menacho I (2023). An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-Src function. Nat Commun 14, 6548. CNIO Publication. Open Access
Mur P, Viana-Errasti J, García-Mulero S, Magraner-Pardo L, Muñoz IG, Pons T, Capellá G, Pineda M, Feliubadaló L, Valle L. (2023). Recommendations for the classification of germline variants in the exonuclease domain of POLE and POLD1. Genome Med 15, 85. CNIO Publication.
Martínez-Caballero S, Freton C, Molina R, Bartual SG, Gueguen-Chaignon V, Mercy C, Gago F, Mahasenan KV, Muñoz IG, Lee M, Hesek D, Mobashery S, Hermoso JA, Grangeasse C. (2023). Molecular basis of the final step of cell division in Streptococcus pneumoniae. Cell Reports 42, 112756. CNIO Publication.
Lázaro-Gorines R, Pérez P, Heras-Murillo I, Adán-Barrientos I, Albericio G, Astorgano D, Flores S, Luczkowiak J, Labiod N, Harwood SL, Segura-Tudela A, Rubio-Pérez L, Nugraha Y, Shang X, Li Y, Alfonso C, Adipietro KA, Abeyawardhane DL, Navarro R, Compte M, Yu W, MacKerell AD Jr, Sanz L, Weber DJ, Blanco FJ, Esteban M, Pozharski E, Godoy-Ruiz R, Muñoz IG, Delgado R, Sancho D, García-Arriaza J, Álvarez-Vallina L. (2023). Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2.. Adv Sci 10, e2304818. CNIO Publication.
Lama R, Xu C, Galster SL, Querol-García J, Portwood S, Mavis CK, Ruiz FM, Martin D, Wu J, Giorgi MC, Bargonetti J, Wang ES, Hernandez-Ilizaliturri FJ, Koudelka GB, Chemler SR, Muñoz IG, Wang X. (2022). Small molecule MMRi62 targets MDM4 for degradation and induces leukemic cell apoptosis regardless of p53 status. Front Oncol 12, 933446. CNIO Publication.
Compte M, Harwood SL, Erce-Llamazares A, Tapia-Galisteo A, Romero E, Ferrer I, Garrido-Martin EM, Enguita AB, Ochoa MC, Blanco B, Oteo M, Merino N, Nehme-Álvarez D, Hangiu O, Domínguez-Alonso C, Zonca M, Ramírez-Fernández A, Blanco FJ, Morcillo MA, Muñoz IG, Melero I, Rodriguez-Peralto JL, Paz-Ares L, Sanz L, Alvarez-Vallina L. (2021). An Fc-free EGFR-specific 4-1BB-agonistic Trimerbody Displays Broad Antitumor Activity in Humanized Murine Cancer Models without Toxicity. Clin Cancer Res 27, 3167-3177. CNIO Publication.
Martínez-Caballero S, Mahasenan KV, Kim C, Molina R, Feltzer R, Lee M, Bouley R, Hesek D, Fisher JF, Muñoz IG, Chang M, Mobashery S, Hermoso JA. (2021). Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery. COMPUT STRUCT BIOTEC 19, 5392-5405. CNIO Publication.
Martínez-González S, García AB, Albarrán MI, Cebriá A, Amezquita-Alves A, García-Campos FJ, Martínez-Gago J, Martínez-Torrecuadrada J, Muñoz IG, Blanco-Aparicio C, Pastor J (2020). Pyrido[2,3-b][1,5]benzoxazepin-5(6H)-one derivatives as CDK8 inhibitors.. Eur J Med Chem 201, 112443. CNIO Publication.
de Salas F, Cañadas R, Santiago G, Virseda-Jerez A, Vind J, Gentili P, Martínez AT, Guallar V, Muñoz IG, Camarero S (2019). Structural and biochemical insights into an engineered high-redox potential laccase overproduced in Aspergillus.. Int J Biol Macromol 141, 855-866. CNIO Publication.