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The primary goal of the Electron Microscopy (EM) Unit is to provide scientific-technical support to researchers in resolving their scientific questions using various transmission EM techniques. We routinely employ cryoEM and negative staining for sample preparation. Additionally, we perform data collection and assist with image processing, including 2D analysis and 3D reconstruction. Support is offered in selecting appropriate EM techniques and preparing samples. Furthermore, we manufacture our own sample supports (EM grids) to ensure better quality control and cost reduction. We also provide the training necessary for the use of our microscopes and auxiliary equipment. More advanced structural studies are typically carried out through research collaborations.
Publications
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- García- Martín C, López-Perrote A, Boskovic J , Llorca O (2024). Mechanism of allosteric inhibition of RUVBL1-RUVBL2 ATPase by the small molecule CB-6644. Cell Rep Phys Sci 5, 101982. CNIO Publication.
- Rivera-Calzada A, Arribas-Bosacoma R, Ruiz-Ramos A, Escudero-Bravo P, Boskovic J, Fernandez-Leiro R, Oliver AW, Pearl LH, Llorca O. (2022). Structural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus.. Nat Commun 13, 7062. CNIO Publication.
Open Access - Rodriguez CF, Escudero-Bravo P, Díaz L, Bartoccioni P, García-Martín C, Gilabert JG, Boskovic J, Guallar V, Errasti-Murugarren E, Llorca O, Palacín M (2021). Structural basis for substrate specificity of heteromeric transporters of neutral amino acids.. Proc Natl Acad Sci USA 118, e2113573118. CNIO Publication.
- Villasante A, Godier-Furnemont A, Hernandez-Barranco A, Coq JL, Boskovic J, Peinado H, Mora J, Samitier J, Vunjak-Novakovic G (2021). Horizontal transfer of the stemness-related markers EZH2 and GLI1 by neuroblastoma-derived extracellular vesicles in stromal cells. Transl Res 237, 82-97. CNIO Publication.
- Le Coq J, López Navajas P, Rodrigo Martin B, Alfonso C, Lietha D (2021). A new layer of phosphoinositide-mediated allosteric regulation uncovered for SHIP2.. FASEB J 35, e21815. CNIO Publication.
- Acebrón I, Righetto RD, Schoenherr C, Buhr S, Redondo P, Culley J, Rodríguez CF, Daday C, Biyani N, Llorca O, Byron A, Chami M, Gräter F, Boskovic J, Margaret C Frame, Stahlberg H, Lietha D (2020). Structural basis of Focal Adhesion Kinase activation on lipid membranes.. EMBO J 39, e104743. CNIO Publication. Open Access
- López-Perrote A , NHug N, González-Corpas A, Rodríguez CF , Serna M, García-Martín C , Boskovic J, Fernandez-Leiro R, Caceres JF , Llorca O (2020). Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife 9, e63042. CNIO Publication. Open Access
- González-Martínez J, Cwetsch AW, Martínez-Alonso D, López-Sainz LR, Almagro J, Megías D, Boskovic J, Gilabert-Juan J, Graña-Castro O, Pierani A, Behrens A, Ortega S, Malumbres M (2020). Lack of adaptation to centriolar defects leads to p53-independent microcephaly in the absence of Cep135. bioRxiv (in press). CNIO Publication.
