The principal mission of the Electron Microscopy (EM) Unit is to provide scientific and technical support mainly to CNIO researchers, helping them address their scientific questions through various transmission electron microscopy techniques. We focus on sample preparation for cryo-electron microscopy (cryoEM) and negative staining methods. Our activity also includes data collection and assistance with image processing, such as 2D analysis and 3D reconstruction. We guide researchers in selecting the most appropriate EM techniques and assist with sample preparation. Additionally, we produce our own EM grids to ensure better quality control and reduce costs. Comprehensive training on the operation of our microscopes and auxiliary equipment is also available. More advanced structural studies are typically conducted through research collaborations.

The Unit has the following equipment:

• Transmition electron microscopes: 120kV Tecnai G2 Spirit (Thermo Fisher) equipped with CCD camera TemCam-F416 (TVIPS) and cryo-holders for one and three samples (Gatan 626 for one sample and Gatan 910 for three samples);
• 200 kV transmition electron microscope, JEM-2200FS (JEOL) with K3 direct electron detector (Gatan), ELSA holder for single sample and 910 holder for three samples (Gatan);
• High-speed sample vitrification prototype (RAPIDO);
• Semi-automated vitrification device Vitrobot Mark IV (Thermo Fisher);
• Glow Discharge GloQube (Quorum Technologies);
• Evaporator E6700 (Polaron);
• Two dewars with four racks (SubAngstrom) for storage cryo-grids in liquid nitrogen.

External users should contact the Head of the Unit via e-mail jboskovic@cnio.es in order to arrange the use of the equipment.