Unidad de Microscopía Electrónica

Jasminka Boskovic Jefe de Unidad
T +34 917328000 (Ext 3080)
jboskovic@cnio.es

Becarios Post-doctorales

Johanne Le Coq

The main objective of the Electron Microscopy (EM) Unit is to provide scientific-technical support to researchers to answer their biological questions using different transmission EM techniques. We regularly use negative staining and cryo-EM and help with image processing by performing 2D analysis and 3D reconstruction. We also offer support for choosing adequate EM techniques and performing sample preparation on different types of EM grids. Furthermore, we provide the necessary training for the use of our microscopes and auxiliary equipment. More advanced studies are typically delivered through research collaboration.

Publicaciones

Rodriguez CF, Escudero-Bravo P, Díaz L, Bartoccioni P, García-Martín C, Gilabert JG, Boskovic J, Guallar V, Errasti-Murugarren E, Llorca O, Palacín M (2021). Structural basis for substrate specificity of heteromeric transporters of neutral amino acids.. Proc Natl Acad Sci USA 118, e2113573118. Publicación CNIO.
Villasante A, Godier-Furnemont A, Hernandez-Barranco A, Coq JL, Boskovic J, Peinado H, Mora J, Samitier J, Vunjak-Novakovic G (2021). Horizontal transfer of the stemness-related markers EZH2 and GLI1 by neuroblastoma-derived extracellular vesicles in stromal cells. Transl Res 237, 82-97. Publicación CNIO.
Le Coq J, López Navajas P, Rodrigo Martin B, Alfonso C, Lietha D (2021). A new layer of phosphoinositide-mediated allosteric regulation uncovered for SHIP2.. FASEB J 35, e21815. Publicación CNIO.
Acebrón I, Righetto RD, Schoenherr C, Buhr S, Redondo P, Culley J, Rodríguez CF, Daday C, Biyani N, Llorca O, Byron A, Chami M, Gräter F, Boskovic J, Margaret C Frame, Stahlberg H, Lietha D (2020). Structural basis of Focal Adhesion Kinase activation on lipid membranes.. EMBO J 39, e104743. Publicación CNIO.
López-Perrote A , NHug N, González-Corpas A, Rodríguez CF , Serna M, García-Martín C , Boskovic J, Fernandez-Leiro R, Caceres JF , Llorca O (2020). Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife 9, e63042. Publicación CNIO. Open Access
González-Martínez J, Cwetsch AW, Martínez-Alonso D, López-Sainz LR, Almagro J, Megías D, Boskovic J, Gilabert-Juan J, Graña-Castro O, Pierani A, Behrens A, Ortega S, Malumbres M (2020). Lack of adaptation to centriolar defects leads to p53-independent microcephaly in the absence of Cep135. bioRxiv (in press). Publicación CNIO.
García-Silva S, Benito-Martín A, Sánchez-Redondo S, Hernández-Barranco A, Ximénez-Embún P, Nogués L, Mazariegos MS, Brinkmann K, Amor López A, Meyer L, Rodríguez C, García-Martín C, Boskovic J, Letón R, Montero C, Robledo M, Santambrogio L, Sue Brady M, Szumera-Cieckiewicz A, Kalinowska I, Skog J, Noerholm M, Muñoz J, Ortiz-Romero PL, Ruano Y, Rodríguez-Peralto JL, Rutkowski P, Peinado H (2019). Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation. J Exp Med 216, 1061-1070. Publicación CNIO.
Bauer MS, Baumann F, Daday C, Redondo P, Durner E, Jobst MA, Milles LF, Mercadante D, Pippig DA, Gaub HE, Gräter F, Lietha D (2019). Structural and mechanistic insights into mechanoactivation of focal adhesion kinase. Proc Natl Acad Sci USA 116, 6766-6774. Publicación CNIO.
Toribio R, Díaz-López I, Boskovic J, Ventoso I (2018). Translation initiation of alphavirus mRNA reveals new insights into the topology of the 48S initiation complex. Nucleic Acids Res 46, 4176-4187. Publicación CNIO. Open Access
Lafarga V, Sirozh O, Diaz-Lopez I, Hisaoka M, Zarzuela E, Boskovic J, Jovanovic B, Fernandez-Leiro R, Munoz J, Stoecklin G, Ventoso I, Fernandez-Capetillo O (2018). DNA and RNA binding mediate the toxicity of arginine-rich peptides encoded by C9ORF72 GGGGCC repeats. bioRxiv (in press). Publicación CNIO.