The primary goal of the Electron Microscopy (EM) Unit is to provide scientific-technical support to researchers in resolving their scientific questions using various transmission EM techniques. We routinely employ cryoEM and negative staining for sample preparation. Additionally, we perform data collection and assist with image processing, including 2D analysis and 3D reconstruction. Support is offered in selecting appropriate EM techniques and preparing samples. Furthermore, we manufacture our own sample supports (EM grids) to ensure better quality control and cost reduction. We also provide the training necessary for the use of our microscopes and auxiliary equipment. More advanced structural studies are typically carried out through research collaborations.
Publicaciones
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García- Martín C, López-Perrote A, Boskovic J , Llorca O (2024).Mechanism of allosteric inhibition of RUVBL1-RUVBL2 ATPase by the small molecule CB-6644. Cell Rep Phys Sci 5, 101982. Publicación CNIO.
González-Martínez J, Cwetsch AW, Martínez-Alonso D, López-Sainz LR, Almagro J, Megías D, Boskovic J, Gilabert-Juan J, Graña-Castro O, Pierani A, Behrens A, Ortega S, Malumbres M (2020).Lack of adaptation to centriolar defects leads to p53-independent microcephaly in the absence of Cep135. bioRxiv (in press). Publicación CNIO.
Open Access