Despite the biochemical complexity of the DNA replication process, the molecular machinery that duplicates our genome displays a remarkable capacity to adapt to different cell types, each one with its own transcriptional programme and specific patterns of chromatin organisation. In addition, the replisome proteins react to endogenous and exogenous factors that induce replicative stress (RS) and may cause DNA breaks, recombination events, and genomic instability. Our Group studies the mechanisms that confer operational flexibility to the replicative process, combining molecular and cellular approaches in human and mouse cells. In 2022, we completed two studies describing the cellular responses to specific situations of stress, which involve the regulation of origin activity and the control over replication fork progression. We also continued to study the dynamics of DNA replication and the impact of RS in other cellular contexts, including the acquisition of metastatic capacity by tumour cells.
- Estrella Guarino
- Susana Llanos
- Sara Rodríguez
- Sergio Muñoz
- Elena Blanco
- Roberto Masdemont
- Sergi Roig
- Patricia Ubieto
- (2023). RHOJ controls EMT-associated resistance to chemotherapy. Nature 616, 168-175. CNIO Publication.
- (2023). miR-28-based combination therapy impairs aggressive B cell lymphoma growth by rewiring DNA replication. Cell Death Dis 14, 687. CNIO Publication.
- (2022). Stress-triggered hematopoietic stem cell proliferation relies on PrimPol-mediated repriming. Mol Cell 38, 4176-4188. CNIO Publication.
- (2022). 3D chromatin connectivity underlies replication origin efficiency in mouse embryonic stem cells. Nucleic Acids Res 50, 12149-12165. CNIO Publication.
- (2022). A truncating variant of RAD51B associated with primary ovarian insufficiency provides insights into its meiotic and somatic functions. Cell Death Differ 29, 2347-2361. CNIO Publication.
- (2022). Regulation of Claspin by the p38 stress-activated protein kinase protects cells from DNA damage. Cell Reports 40, 111375. CNIO Publication.
- (2021). Motif WFYY of human PrimPol is crucial to stabilize the incoming 3′-nucleotide during replication fork restart. Nucleic Acids Res 49, 8199-8213. CNIO Publication. Open Access
- (2021). PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. EMBO J 40, e106355. CNIO Publication.
- (2020). PRIMPOL-Mediated Adaptive Response Suppresses Replication Fork Reversal in BRCA-Deficient Cells.. Mol Cell 77, 461-474. CNIO Publication.
- (2020). PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts. Nat Commun 11, 5863. CNIO Publication. Open Access
- (2020). PDS5 proteins are required for proper cohesin dynamics and participate in replication fork protection. J Biol Chem 295, 146-157. CNIO Publication. Open Access
- (2019). TIAR controls mitotic entry, maintains genome stability and retains CDK1 in checkpoint bodies.. EMBO Rep 20, pii: e46224. CNIO Publication.
- (2019). Identification and characterization of Cardiac Glycosides as senolytic compounds.. Nat Commun 10, 4731. CNIO Publication. Open Access
- (2019). TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress. EMBO Rep 20, e46224. CNIO Publication.
- (2019). Lysosomal trapping of palbociclib and its functional implications. Oncogene 38, 3886-3902. CNIO Publication.
- (2019). A cancer-associated point mutation disables the steric gate of human PrimPol. Sci Rep 9, 1121. CNIO Publication. Open Access
- (2019). Three-dimensional connectivity and chromatin environment mediate the activation efficiency of mammalian DNA replication origins.. bioRxiv (in press). CNIO Publication.
- (2018). Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19, e45770. CNIO Publication.
- (2018). Uncoupling fork speed and origin activity to identify the primary cause of replicative stress phenotypes. J Biol Chem 293, 12855-12861. CNIO Publication.