Flow Cytometry Core Unit

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Technicians

  • Sara García
  • Julia García

Flow Cytometry is a fast and multiparametric technology, and an extremely valuable tool in the oncology field. It is an important workhorse able to identify, quantify and isolate defined subpopulations of cells, based on the levels of expression of fluorescent markers and their relation to each other at the single cell level.

Our aim is to provide CNIO Groups with technical and scientific advice regarding the use of cytometric technologies, collaborating with them in the design, acquisition, data analysis, and interpretation of flow cytometry data.

We currently have 4 analysers and 3 high-speed cell sorters with different optical configurations to cater to users’ needs. We also have an automated magnetic bead separation system (AutoMACS), 2 automated cell counters (Countess) and a tissue homogenizer (GentleMACS). Analysers are user-operated upon appropriate training, and cell sorters are operated by the Unit staff. Our sorters can separate up to 4- or 6- defined populations simultaneously, as well as perform single cell cloning and index sorting. We can accept human samples to sort under BSL2 regulations.

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