- Sara García
- Julia García
Flow Cytometry is a fast and multiparametric technology, and an extremely valuable tool in the oncology field. It is an important workhorse able to identify, quantify and isolate defined subpopulations of cells, based on the levels of expression of fluorescent markers and their relation to each other at the single cell level.
Our aim is to provide CNIO Groups with technical and scientific advice regarding the use of cytometric technologies, collaborating with them in the design, acquisition, data analysis, and interpretation of flow cytometry data.
We currently have 4 analysers and 3 high-speed cell sorters with different optical configurations to cater to users’ needs. We also have an automated magnetic bead separation system (AutoMACS), 2 automated cell counters (Countess) and a tissue homogenizer (GentleMACS). Analysers are user-operated upon appropriate training, and cell sorters are operated by the Unit staff. Our sorters can separate up to 4- or 6- defined populations simultaneously, as well as perform single cell cloning and index sorting. We can accept human samples to sort under BSL2 regulations.
- (2020). miR-34c-3p targets CDK1 a synthetic lethality partner of KRAS in non-small cell lung cancer. Cancer Gene Ther (in press). CNIO Publication.
- (2020). Biosafety during a pandemic: shared resource laboratories rise to the challenge.. Cytometry (in press). CNIO Publication.
- (2018). Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19, e45770. CNIO Publication.
- (2018). STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis. Nat Med 24, 1024-1035. CNIO Publication.
- (2018). Sirt1 protects from K-Ras-driven lung carcinogenesis. EMBO Rep 19, e43879. CNIO Publication.
- (2017). Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation. J Exp Med 214, 1387-1409. CNIO Publication.
- (2017). Analysis of the advantages of cis reporters in optimized FACS-Gal. Cytom Part A 91, 721-729. CNIO Publication.
- (2016). p21Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARa. Sci Rep 6, 34542. CNIO Publication. Open Access