Unidad de Citometría de Flujo

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Lola Martinez

Técnicos de Laboratorio

  • Irene Fernández
  • Julia García

Flow cytometry is a fast and multiparametric technology, indispensable in the field of cancer. It allows for the identification, quantification and isolation of defined subpopulations, based on the expression levels of fluorescent markers at the single cell level, as well as the quantitative measurement of cell proliferation and cell death rates upon genetic modifications or drug treatments.

Our aim is to provide CNIO Groups with technical and scientific advice on the use of flow cytometry needed to answer their biological questions, developing new methodologies or pipelines when needed.

We currently have 4 analysers and 3 high-speed cell sorters with different optical configurations to cater to user needs, plus other small instruments to help standardise sample preparation. We offer extensive training so users can independently operate the analysers, while the Unit staff operate the cell sorters, which can separate up to 4- or 6- defined populations simultaneously as well as perform single cell cloning and index sorting. We can accept human samples to sort under BSL2 regulations.

Publicaciones

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  • Barker-Tejeda TC, Zubeldia-Varela E, Macías-Camero A, Alonso L, Martín-Antoniano IA, Rey-Stolle MF, Mera-Berriatua L, Bazire R, Cabrera-Freitag P, Shanmuganathan M, Britz-McKibbin P, Ubeda C, Francino MP, Barber D, Ibáñez-Sandín MD, Barbas C, Pérez-Gordo M, Villaseñor A. (2024). Comparative characterization of the infant gut microbiome and their maternal lineage by a multi-omics approach. Nat Commun 15, 3004. Publicación CNIO.
  • Perez-Gonzalez A, Lopes T, Martinez L, Bispo C, Gardner R, Riddell A. (2024). Evaluation of Sort Recovery via Rmax. Curr Protocols 4, e986. Publicación CNIO.
  • Back JB, Martinez L, Nettenstrom L, Sheerar D, Thornton S (2022). Establishing a biosafety plan for a flow cytometry shared resource laboratory. Cytom Part A 101, 380-386. Publicación CNIO.
  • Palma F, Affinito A, Nuzzo S, Roscigno G, Scognamiglio I, Ingenito F, Martinez L, Franzese M, Zanfardino M, Soricelli A, Fiorelli A, Condorelli G, Quintavalle C (2020). miR-34c-3p targets CDK1 a synthetic lethality partner of KRAS in non-small cell lung cancer. Cancer Gene Ther (in press). Publicación CNIO.
  • Aspland AM, Douagi I, Filby A, Jellison ER, Martinez L, Shinko D, Smith AL, Tang VA, Thornton S (2020). Biosafety during a pandemic: shared resource laboratories rise to the challenge.. Cytometry (in press). Publicación CNIO. Open Access Open Access
  • Daniels K, Conway A, Gardner R, Martinez L, Price KM, Schneider S, Sheridan R, Srivastava J, Thornton S (2020). Remote Training of SRL Users and Staff in a Global Pandemic. Cytometry (in press). Publicación CNIO. Open Access Open Access

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