Flow Cytometry is a fast and multiparametric technology, and a very valuable tool in the oncology field. It is an important workhorse for the identification, quantification and isolation of defined subpopulations of cells, based on the expression levels of fluorescent markers and their relationship to each other at the single cell level.
Our aim is to provide CNIO Groups with technical and scientific advice regarding the use of cytometric technologies, collaborating with them in the design, acquisition, data analysis, and interpretation of flow cytometry data.
We currently have 5 analysers and 3 high-speed cell sorters with different optical configurations to cater to our users’ needs. We also have an automated magnetic bead separation system (AutoMACS), 2 automated cell counters (Countess) and a tissue homogenizer (GentleMACS). Analysers are user-operated upon appropriate training, and cell sorters are operated by the Unit staff. Our sorters can separate up to 4- or 6- defined populations simultaneously, as well as perform single cell cloning and index sorting. We can accept human samples to sort under BSL2 regulations.