Flow Cytometry is a fast, multiparametric technology indispensable in the oncology field. It allows for the identification, quantification, and isolation of defined subpopulations based on the expression levels of fluorescent markers at the single cell level.
Our aim is to provide CNIO groups with technical and scientific advice on the use of flow cytometry to answer their biological questions.
We currently have 4 analysers and 3 high-speed cell sorters with different optical configurations, plus other small instruments to help standardise sample preparation. We offer extensive training so users can independently operate analysers, while Unit staff operate cell sorters, which can separate up to 4- or 6- defined populations simultaneously, depending on the nozzle type, as well as perform single cell cloning and index sorting. We can accept human samples for sorting under BSL-2 regulations.
Publicaciones recientes
Hergueta-Redondo M, Sánchez-Redondo S, Hurtado B, Santos V, Pérez-Martínez M, Ximénez-Embún P, McDowell SAC, Mazariegos MS, Mata G, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Graña-Castro O, Megias D, Quail D, Quintela-Fandino M, Peinado H. (2025).The impact of a high fat diet and platelet activation on pre-metastatic niche formation. Nat Commun 16, 2897. Publicación CNIO.
