Flow cytometry is a fast and multiparametric technology, indispensable in the field of cancer. It allows for the identification, quantification and isolation of defined subpopulations, based on the expression levels of fluorescent markers at the single cell level, as well as to measure quantitatively cell proliferation and cell death rates upon genetic modifications or drug treatments.
Our aim is to provide CNIO Groups with technical and scientific advice on the use of flow cytometry needed to answer their biological questions, developing new methodologies or pipelines when needed.
We currently have 4 analysers and 3 high-speed cell sorters with different optical configurations to cater to user needs, plus other small instruments to help standardise sample preparation. We offer extensive training so users can independently operate the analysers, while the Unit staff operate the cell sorters, which can separate up to 4- or 6- defined populations simultaneously, depending on the nozzle type, as well as perform single cell cloning and index sorting. We can accept human samples to sort under BSL2 regulations.
Publicaciones recientes
Hergueta-Redondo M, Sánchez-Redondo S, Hurtado B, Santos V, Pérez-Martínez M, Ximénez-Embún P, McDowell SAC, Mazariegos MS, Mata G, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Graña-Castro O, Megias D, Quail D, Quintela-Fandino M, Peinado H. (2025).The impact of a high fat diet and platelet activation on pre-metastatic niche formation. Nat Commun 16, 2897. Publicación CNIO.
