Unidad de Citometría de Flujo

Lola Martinez Jefe de Unidad
T +34 917328000 (Ext 3210)
lmartinez@cnio.es

Técnicos de Laboratorio

Sara García
Julia García
Marina Quesada

Flow Cytometry is an indispensable tool in the oncology field. It allows multiparametric analysis for the identification, quantification and isolation of defined subpopulations of cells, based on the levels of expression of fluorescent markers and their relation to each other.

Our aim is to provide CNIO groups with technical and scientific advice regarding the use of cytometric technologies, collaborating with them in the design, acquisition, data analysis and interpretation.

We have 4 analysers and 3 high-speed cell sorters, with different configurations of lasers and detectors, to cater to all our users’ needs. We also have an automated magnetic bead separation system (AutoMACS), 2 automated cell counters and a tissue homogeniser (GentleMACS). Analysers are user-operated upon appropriate training, and cell sorters are operated by the Unit staff. Our sorters can separate up to 4- or 6- defined populations simultaneously, as well as perform single cell cloning. We can accept human samples to sort according to Biosafety regulations.

Publicaciones

Pérez-Olivares M, Trento A, Rodriguez-Acebes S, González-Acosta D, Fernández-Antorán D, Román-García S, Martinez D, López-Briones T, Torroja C, Carrasco YR, Méndez J, Moreno de Alborán I (2018). Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19, e45770. Publicación CNIO.
Priego N, Zhu L, Monteiro C, Mulders M, Wasilewski D, Bindeman W, Doglio L, Martínez L, Martínez-Saez E, Cajal SRY, Megías D, Hernández-Encinas E, Blanco-Aparicio C, Martínez L, Zarzuela E, Muñoz J, Fustero-Torres C, Pineiro E, Hernández-Laín A, Bertero L, Poli V, Sánchez-Martínez M, Menendez JA, Soffietti R, Bosch-Barrera J, Valiente M (2018). STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis. Nat Med 24, 1024-1035. Publicación CNIO.
Costa-Machado LF, Martín-Hernández R, Sanchez-Luengo MÁ, Hess K, Vales-Villamarin C, Barradas M, Lynch C, de la Nava D, Diaz-Ruiz A, de Cabo R, Cañamero M, Martinez L, Sanchez-Carbayo M, Herranz D, Serrano M, Fernandez-Marcos PJ (2018). Sirt1 protects from K-Ras-driven lung carcinogenesis. EMBO Rep 19, e43879. Publicación CNIO.
Bakiri L, Hamacher R, Graña O, Guío-Carrión A, Campos-Olivas R, Martinez L, Dienes HP, Thomsen MK, Hasenfuss SC, Wagner EF (2017). Liver carcinogenesis by FOS-dependent inflammation and cholesterol dysregulation. J Exp Med 214, 1387-1409. Publicación CNIO.
Sánchez-Luengo MA, Rovira M, Serrano M, Fernández-Marcos PJ, Martínez L (2017). Analysis of the advantages of cis reporters in optimized FACS-Gal. Cytom Part A 91, 721-729. Publicación CNIO.
López-Guadamillas E, Fernández-Marcos PJ, Pantoja C, Muñoz-Martin M, Martínez D, Gómez-López G, Campos-Olivas R, Valverde AM, Serrano M (2016). p21Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARa. Sci Rep 6, 34542. Publicación CNIO. Open Access
Cioffi M, Vallespinos-Serrano M, Trabulo SM, Fernandez-Marcos PJ, Firment AN, Vazquez BN, Vieira CR, Mulero F, Camara JA, Cronin UP, Perez M, Soriano J, G Galvez B, Castells-Garcia A, Haage V, Raj D, Megias D, Hahn S, Serrano L, Moon A, Aicher A, Heeschen C (2015). MiR-93 Controls Adiposity via Inhibition of Sirt7 and Tbx3. Cell Reports 12, 1594-1605. Publicación CNIO. Open Access
Alvarez S, Díaz M, Flach J, Rodriguez-Acebes S, López-Contreras AJ, Martínez D, Cañamero M, Fernández-Capetillo O, Isern J, Passegué E, Méndez J (2015). Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nat Commun 6, 8548. Publicación CNIO. Open Access
Riddell A, Gardner R, Perez-Gonzalez A, Lopes T, Martinez L (2015). Rmax: A systematic approach to evaluate instrument sort performance using center stream catch.. Methods 82, 64-73. Publicación CNIO.
Morgado-Palacin L, Vareti G, Llanos S, Gomez-Lopez G, Martinez D, Serrano M (2015). Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis. Cell Reports 13, 712-722. Publicación CNIO. Open Access