Orlando Domínguez Head of Unit
T +34 917328000 (Ext 3120)
odominguez@cnio.es

The Genomics Unit was established in 2000 for the purpose of providing the cutting-edge technology and services required by the CNIO research community. With the capacity to even interrogate whole genomes in a single assay, technologies such as high-density DNA microarrays and next-generation sequencing (NGS) reveal the genetic diversity of cancer and help to dissect molecular processes. Structural features, such as mutation repertoires, DNA-binding of protein factors, variations in chromatin structure/folding, as well as functional states such as transcriptomic profiles and changes (mRNA, miRNA) are being elucidated with these technologies in order to uncover basic mechanisms, therapeutic targets and prognostic biomarkers. We offer a broad range of products and services, including microarray whole genome gene expression, array comparative genomic hybridisation (aCGH), NGS library preparation for exome sequencing, ChIPseq and RNAseq analysis, transgenic mouse genotyping, human cell line authentication and capillary DNA sequencing.

Publications

• Exome sequencing of plasma DNA portrays the mutation landscape of colorectal cancer and discovers mutated VEGFR2 receptors as modulators of anti-angiogenic therapies.(2018)
  Toledo RA, Garralda E, Mitsi M, Pons T, Monsech J, Vega E, Otero A, Albarran MI, Baños N, Duran Y, Bonilla V, Sarno F, Camacho Artacho M, Sánchez-Pérez T, Perea S, Alvarez R, De Martino Rodriguez A, Lietha D, Blanco-Aparicio C, Cubillo A, Dominguez O, Martínez-Torrecuadrada J, Hidalgo M.
  Clin Cancer Res (in press).
• Exome Sequencing of Plasma DNA Portrays the Mutation Landscape of Colorectal Cancer and Discovers Mutated VEGFR2 Receptors as Modulators of Antiangiogenic Therapies(2018)
  Toledo RA, Garralda E, Mitsi M, Pons T, Monsech J, Vega E, Otero Á, Albarran MI, Baños N, Durán Y, Bonilla V, Sarno F, Camacho-Artacho M, Sanchez-Perez T, Perea S, Álvarez R, De Martino A, Lietha D, Blanco-Aparicio C, Cubillo A, Domínguez O, Martínez-Torrecuadrada JL, Hidalgo M
  Clin Cancer Res 24, 3550-3559.
• ERF deletion rescues RAS deficiency in mouse embryonic stem cells.(2018)
  Mayor-Ruiz C, Olbrich T, Drosten M, Lecona E, Vega-Sendino M, Ortega S, Dominguez O, Barbacid M, Ruiz S, Fernandez-Capetillo O.
  Genes Dev 32, 568-576.
• TrapSeq: An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells.(2017)
  Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O
  J Mol Biol 429, 2780-2789.
• Profiling of Sox4-dependent transcriptome in skin links tumour suppression and adult stem cell activation(2015)
  Foronda M, Morgado-Palacin L, Gómez-López G, Domínguez O, Pisano DG, Blasco MA
  GENOMICS DATA 6, 21-24.
• The pluripotency factor NANOG promotes the formation of squamous cell carcinomas.(2015)
  Palla AR, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez-López G, Dominguez O, Dueñas M, Paramio JM, Serrano M.
  Sci Rep 5, 10205-.
• Reprogramming activity of NANOGP8, a NANOG family member widely expressed in cancer.(2014)
  Palla AR, Piazzolla D, Abad M, Li H, Dominguez O, Schonthaler HB, Wagner EF, Serrano M
  Oncogene 33, 2513-2519.
• Identification of TERRA locus unveils a telomere protection role through association to nearly all chromosomes.(2014)
  de Silanes IL, Graña O, De Bonis ML, Dominguez O, Pisano DG, Blasco MA
  Nat Communications 5, 4723-.
• Genome-wide analysis of in vivo TRF1 binding to chromatin restricts its location exclusively to telomeric repeats.(2014)
  Garrobo I, Marión RM, Domínguez O, Pisano DG, Blasco MA
  Cell Cycle 13, 3742-3749.
• Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia.(2014)
  Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, Luque-Garcia JL, Fernandez-Tresguerres B, Fernandez AF, Fraga MF, Rodriguez-Justo M, Manzanares M, Sánchez-Carbayo M, García-Pedrero JM, Rodrigo JP, Malumbres M, Serrano M
  Nat Communications 5, 4226-.
• PLCG1 mutations in cutaneous T-cell lymphomas.(2014)
  Vaqué JP, Gómez-López G, Monsálvez V, Varela I, Martínez N, Pérez C, Domínguez O, Graña O, Rodríguez-Peralto JL, Rodríguez-Pinilla SM, González-Vela C, Rubio-Camarillo M, Martín-Sánchez E, Pisano DG, Papadavid E, Papadaki T, Requena L, García-Marco JA, Méndez M, Provencio M, Hospital M, Suárez-Massa D, Postigo C, San Segundo D, López-Hoyos M, Ortiz-Romero PL, Piris MA, Sánchez-Beato M.
  Blood 123, 2034-2043.
• Exome sequencing of three cases of familial exceptional longevity.(2014)
  Cash TP1, Pita G, Domínguez O, Alonso MR, Moreno LT, Borrás C, Rodríguez-Mañas L, Santiago C, Garatachea N, Lucia A, Avellana JA, Viña J, González-Neira A, Serrano M
  Aging Cell 13, 1087-1090.
• Whole exome sequencing suggests much of non-BRCA1/BRCA2 familial breast cancer is due to moderate and low penetrance susceptibility alleles.(2013)
  Gracia-Aznarez FJ, Fernandez V, Pita G, Peterlongo P, Dominguez O, de la Hoya M, Duran M, Osorio A, Moreno L, Gonzalez-Neira A, Rosa-Rosa JM, Sinilnikova O, Mazoyer S, Hopper J, Lazaro C, Southey M, Odefrey F, Manoukian S, Catucci I, Caldes T, Lynch HT, Hilbers FS, van Asperen CJ, Vasen HF, Goldgar D, Radice P, Devilee P, Benitez J
  PLoS ONE 8, e55681-.
• Recurrent inactivation of STAG2 in bladder cancer is not associated with aneuploidy.(2013)
  Balbás-Martínez C, Sagrera A, Carrillo-de-Santa-Pau E, Earl J, Márquez M, Vazquez M, Lapi E, Castro-Giner F, Beltran S, Bayés M, Carrato A, Cigudosa JC, Domínguez O, Gut M, Herranz J, Juanpere N, Kogevinas M, Langa X, López-Knowles E, Lorente JA, Lloreta J, Pisano DG, Richart L, Rico D, Salgado RN, Tardón A, Chanock S, Heath S, Valencia A, Losada A, Gut I, Malats N, Real FX
  Nat Genet 45, 1464-1469.
• ARID1A alterations are associated with FGFR3-wild type, poor-prognosis, urothelial bladder tumors.(2013)
  Balbás-Martínez C, Rodríguez-Pinilla M, Casanova A, Domínguez O, Pisano DG, Gómez G, Lloreta J, Lorente JA, Malats N, Real FX
  PLoS ONE 8, e62483-.
• Reprogramming in vivo produces teratomas and iPS cells with totipotency features.(2013)
  Abad M, Mosteiro Ll, Pantoja C, Cañamero M, Rayon T, Ors I, Graña O, Megías D, Domínguez O, Martinez D, Manzanares M, Ortega S, Serrano M
  Nature 502, 340-345.