Orlando Domínguez Head of Unit
T +34 917328000 (Ext 3120)
odominguez@cnio.es

The Genomics Unit provides on-demand scientific services to the CNIO research community. Cutting-edge technologies have the capacity to interrogate whole genomes in a single assay, such as next-generation sequencing (NGS). These methodologies reveal the genetic diversity of cancer and contribute to dissect its molecular processes. Structural features, such as mutation landscapes, DNA-binding of protein factors, variations in chromatin structure, as well as functional activation states reflected on changes of transcriptomic profiles (mRNA, miRNA), are being elucidated with these technologies in order to uncover basic mechanisms, therapeutic targets and prognostic biomarkers. We offer a broad range of applications, including powerful solutions such as exome mutational landscapes, protein location analysis by ChIP-seq analysis and transcriptome profiles by RNA-seq technologies, besides from the more traditional microarray platform − suitable for whole genome gene expression, array comparative genomic hybridisation (aCGH) − and capillary DNA sequencing. Among other side activities, we also provide a very active transgenic mouse genotyping service.

Publications

• Toledo RA, Garralda E, Mitsi M, Pons T, Monsech J, Vega E, Otero Á, Albarran MI, Baños N, Durán Y, Bonilla V, Sarno F, Camacho-Artacho M, Sanchez-Perez T, Perea S, Álvarez R, De Martino A, Lietha D, Blanco-Aparicio C, Cubillo A, Domínguez O, Martínez-Torrecuadrada JL, Hidalgo M (2018). Exome Sequencing of Plasma DNA Portrays the Mutation Landscape of Colorectal Cancer and Discovers Mutated VEGFR2 Receptors as Modulators of Antiangiogenic Therapies. Clin Cancer Res 24, 3550-3559.
• Mayor-Ruiz C, Olbrich T, Drosten M, Lecona E, Vega-Sendino M, Ortega S, Dominguez O, Barbacid M, Ruiz S, Fernandez-Capetillo O (2018). ERF deletion rescues RAS deficiency in mouse embryonic stem cells. Genes Dev 32, 568-576.
• Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O (2017). TrapSeq: An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells.. J Mol Biol 429, 2780-2789.
• Foronda M, Morgado-Palacin L, Gómez-López G, Domínguez O, Pisano DG, Blasco MA (2015). Profiling of Sox4-dependent transcriptome in skin links tumour suppression and adult stem cell activation. GENOMICS DATA 6, 21-24.
• Palla AR, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez-López G, Dominguez O, Dueñas M, Paramio JM, Serrano M. (2015). The pluripotency factor NANOG promotes the formation of squamous cell carcinomas.. Sci Rep 5, 10205.
• Palla AR, Piazzolla D, Abad M, Li H, Dominguez O, Schonthaler HB, Wagner EF, Serrano M (2014). Reprogramming activity of NANOGP8, a NANOG family member widely expressed in cancer.. Oncogene 33, 2513-2519.
• de Silanes IL, Graña O, De Bonis ML, Dominguez O, Pisano DG, Blasco MA (2014). Identification of TERRA locus unveils a telomere protection role through association to nearly all chromosomes.. Nat Communications 5, 4723.
• Garrobo I, Marión RM, Domínguez O, Pisano DG, Blasco MA (2014). Genome-wide analysis of in vivo TRF1 binding to chromatin restricts its location exclusively to telomeric repeats.. Cell Cycle 13, 3742-3749.
• Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, Luque-Garcia JL, Fernandez-Tresguerres B, Fernandez AF, Fraga MF, Rodriguez-Justo M, Manzanares M, Sánchez-Carbayo M, García-Pedrero JM, Rodrigo JP, Malumbres M, Serrano M (2014). Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia.. Nat Communications 5, 4226.
• Vaqué JP, Gómez-López G, Monsálvez V, Varela I, Martínez N, Pérez C, Domínguez O, Graña O, Rodríguez-Peralto JL, Rodríguez-Pinilla SM, González-Vela C, Rubio-Camarillo M, Martín-Sánchez E, Pisano DG, Papadavid E, Papadaki T, Requena L, García-Marco JA, Méndez M, Provencio M, Hospital M, Suárez-Massa D, Postigo C, San Segundo D, López-Hoyos M, Ortiz-Romero PL, Piris MA, Sánchez-Beato M. (2014). PLCG1 mutations in cutaneous T-cell lymphomas.. Blood 123, 2034-2043.
• Cash TP1, Pita G, Domínguez O, Alonso MR, Moreno LT, Borrás C, Rodríguez-Mañas L, Santiago C, Garatachea N, Lucia A, Avellana JA, Viña J, González-Neira A, Serrano M (2014). Exome sequencing of three cases of familial exceptional longevity.. Aging Cell 13, 1087-1090.
• Gracia-Aznarez FJ, Fernandez V, Pita G, Peterlongo P, Dominguez O, de la Hoya M, Duran M, Osorio A, Moreno L, Gonzalez-Neira A, Rosa-Rosa JM, Sinilnikova O, Mazoyer S, Hopper J, Lazaro C, Southey M, Odefrey F, Manoukian S, Catucci I, Caldes T, Lynch HT, Hilbers FS, van Asperen CJ, Vasen HF, Goldgar D, Radice P, Devilee P, Benitez J (2013). Whole exome sequencing suggests much of non-BRCA1/BRCA2 familial breast cancer is due to moderate and low penetrance susceptibility alleles.. PLoS ONE 8, e55681.
• Balbás-Martínez C, Sagrera A, Carrillo-de-Santa-Pau E, Earl J, Márquez M, Vazquez M, Lapi E, Castro-Giner F, Beltran S, Bayés M, Carrato A, Cigudosa JC, Domínguez O, Gut M, Herranz J, Juanpere N, Kogevinas M, Langa X, López-Knowles E, Lorente JA, Lloreta J, Pisano DG, Richart L, Rico D, Salgado RN, Tardón A, Chanock S, Heath S, Valencia A, Losada A, Gut I, Malats N, Real FX (2013). Recurrent inactivation of STAG2 in bladder cancer is not associated with aneuploidy.. Nat Genet 45, 1464-1469.
• Balbás-Martínez C, Rodríguez-Pinilla M, Casanova A, Domínguez O, Pisano DG, Gómez G, Lloreta J, Lorente JA, Malats N, Real FX (2013). ARID1A alterations are associated with FGFR3-wild type, poor-prognosis, urothelial bladder tumors.. PLoS ONE 8, e62483.
• Abad M, Mosteiro Ll, Pantoja C, Cañamero M, Rayon T, Ors I, Graña O, Megías D, Domínguez O, Martinez D, Manzanares M, Ortega S, Serrano M (2013). Reprogramming in vivo produces teratomas and iPS cells with totipotency features.. Nature 502, 340-345.