Orlando Domínguez Head of Unit
T +34 917328000 (Ext 3120)
odominguez@cnio.es

The Genomics Unit provides on-demand scientific services to the CNIO research community. Cutting-edge technologies have the capacity to interrogate whole genomes in a single assay, such as next-generation sequencing (NGS). These methodologies reveal the genetic diversity of cancer and contribute to dissect its molecular processes. Structural features, such as mutation landscapes, DNA-binding of protein factors, variations in chromatin structure, as well as functional activation states reflected on changes of transcriptomic profiles (mRNA, miRNA), are being elucidated with these technologies in order to uncover basic mechanisms, therapeutic targets and prognostic biomarkers. We offer a broad range of applications, including powerful solutions such as exome mutational landscapes, protein location analysis by ChIP-seq analysis and transcriptome profiles by RNA-seq technologies, besides from the more traditional microarray platform, suitable for whole genome gene expression, array comparative genomic hybridisation (aCGH), and capillary DNA sequencing. Among other side activities, we also provide a very active transgenic mouse genotyping service.

Publications

• Santos CP, Lapi E, Martínez de Villarreal J, Álvaro-Espinosa L, Fernández-Barral A, Barbáchano A, Domínguez O, Laughney AM, Megías D, Muñoz A, Real FX (2019). Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity. Nat Commun 10, 4407. CNIO Publication. Open Access
• Toledo RA, Garralda E, Mitsi M, Pons T, Monsech J, Vega E, Otero Á, Albarran MI, Baños N, Durán Y, Bonilla V, Sarno F, Camacho-Artacho M, Sanchez-Perez T, Perea S, Álvarez R, De Martino A, Lietha D, Blanco-Aparicio C, Cubillo A, Domínguez O, Martínez-Torrecuadrada JL, Hidalgo M (2018). Exome Sequencing of Plasma DNA Portrays the Mutation Landscape of Colorectal Cancer and Discovers Mutated VEGFR2 Receptors as Modulators of Antiangiogenic Therapies. Clin Cancer Res 24, 3550-3559. CNIO Publication.
• Mayor-Ruiz C, Olbrich T, Drosten M, Lecona E, Vega-Sendino M, Ortega S, Dominguez O, Barbacid M, Ruiz S, Fernandez-Capetillo O (2018). ERF deletion rescues RAS deficiency in mouse embryonic stem cells. Genes Dev 32, 568-576. CNIO Publication. Open Access
• Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O (2017). TrapSeq: An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells.. J Mol Biol 429, 2780-2789. CNIO Publication.
• Palla AR, Piazzolla D, Alcazar N, Cañamero M, Graña O, Gómez-López G, Dominguez O, Dueñas M, Paramio JM, Serrano M. (2015). The pluripotency factor NANOG promotes the formation of squamous cell carcinomas.. Sci Rep 5, 10205. CNIO Publication.
• Foronda M, Morgado-Palacin L, Gómez-López G, Domínguez O, Pisano DG, Blasco MA (2015). Profiling of Sox4-dependent transcriptome in skin links tumour suppression and adult stem cell activation. GENOMICS DATA 6, 21-24. CNIO Publication.