Grupo de Microambiente y Metástasis

Hector Peinado Jefe de Grupo
T +34 (+34) 917 328 000 (Ext 3520)
hpeinado@cnio.es

Becarios Post-doctorales

Marta Hergueta

Becarios Pre-Doctorales

Enrique Bastón
María Victoria del Castillo
Juan García-Agulló
Lucía Garrido

Técnicos de Laboratorio

Sara Sánchez-Redondo
Vanesa Santos

Our Laboratory focuses on elucidating the crosstalk between tumours and their microenvironment throughout metastatic progression. Currently, we are examining the relationship between extracellular vesicles (EVs) and DNA damage responses (DDR). This project aims to uncover the potential role of EVs in controlling intrinsic/extrinsic DDR and their impact on tumour evolution and metastasis. Additionally, we are investigating the influence of obesity on pre-metastatic niche formation in breast cancer, with a specific emphasis on its adverse outcomes in triple-negative breast cancer patients. This study will help us to understand the relationship between obesity and the progression of breast cancer. Lastly, we are analysing the role of the nerve growth factor receptor (NGFR) in metastasis and therapy resistance in melanoma and head and neck cancer. We are interested in exploring the use NGFR inhibitors in combination with immunotherapy and chemotherapy to enhance therapeutic responses and reduce metastasis.

Publicaciones

Casanova-Salas I, Aguilar D, Cordoba-Terreros S, Agundez L, Brandariz J, Herranz N, Mas A, Gonzalez M, Morales-Barrera R, Sierra A, Soriano-Navarro M, Cresta P, Mir G, Simonetti S, Rodrigues G, Arce-Gallego S, Delgado-Serrano L, Agustí I, Castellano-Sanz E, Mast R, de Albert M, Celma A, Santamaria A, Gonzalez L, Castro N, Suanes MDM, Hernández-Losa J, Nonell L, Peinado H, Carles J, Mateo J. (2024). Circulating tumor extracellular vesicles to monitor metastatic prostate cancer genomics and transcriptomic evolution. Cancer Cell 42, 1301-1312. Publicación CNIO.
Quiralte M, Barquín A, Yagüe-Fernández M, Navarro P, Grazioso TP, Sevillano-Fernández E, Rodriguez-Moreno JF, Balarezo-Saldivar A, Peinado H, Izquierdo E, Millán C, López-Carrasco I, Prieto M, Madurga R, Fernández-Miranda I, Ruiz-Llorente S, García-Donas J (2024). Proteomic profiles of peritoneal fluid-derived small extracellular vesicles correlate with patient outcome in ovarian cancer. J Clin Invest 134, e176161. Publicación CNIO.
Hernández-Barranco A, Santos V, Mazariegos MS, Caleiras E, Nogués L, Mourcin F, Léonard S, Oblet C, Genebrier S, Rossille D, Benguría A, Sanz A, Vázquez E, Dopazo A, Efeyan A, Ortega-Molina A, Cogne M, Tarte K, Peinado H. (2024). NGFR regulates stromal cell activation in germinal centers. Cell Reports 43, 113705. Publicación CNIO. Open Access
García-Silva S, Peinado H. (2024). Mechanisms of lymph node metastasis: An extracellular vesicle perspective. Eur J Cell Biol 103, 151447. Publicación CNIO.
Vidal-Correoso D, Mateo SV, Muñoz-Morales AM, Lucas-Ruiz F, Jover-Aguilar M, Alconchel F, Martínez-Alarcón L, Sánchez-Redondo S, Santos V, López-López V, Ríos-Zambudio A, Cascales P, Pons JA, Ramírez P, Pelegrín P, Peinado H, Baroja-Mazo A. (2024). Cell-specific Extracellular Vesicles and Their miRNA Cargo Released Into the Organ Preservation Solution During Cold Ischemia Storage as Biomarkers for Liver Transplant Outcomes. Transplantation (in press). Publicación CNIO.
Heidinger M, Egle D, Piscuoglio S, Navarro-Aguadero MÁ, Sánchez S, Hergueta-Redondo M, Gallardo M, Barrio S, García-Peláez B, Molina-Vila MA, Maggi N, Eller RS, Loesch JM, Alborelli I, Peinado H, Weber W, Weber WP. (2024). Extracellular Vesicle DNA Extraction and Sequencing in Ancient Serum Samples From Patients With Breast Cancer. Anticancer Res 44, 2981-2988. Publicación CNIO.
González-Muñoz T, Di Giannatale A, García-Silva S, Santos V, Sánchez-Redondo S, Savini C, Graña-Castro O, Blanco-Aparicio C, Fischer S, De Wever O, Creus-Bachiller E, Ortega-Bertran S, Pisapia DJ, Rodríguez-Peralto JL, Fernández-Rodríguez J, Pérez-Portabella CR, Alaggio R, Benassi MS, Pazzaglia L, Scotlandi K, Ratner N, Yohay K, Theuer CP, Peinado H. (2023). Endoglin, a Novel Biomarker and Therapeutical Target to Prevent Malignant Peripheral Nerve Sheath Tumor Growth and Metastasis. Clin Cancer Res 29, 3744-3758. Publicación CNIO.
García-Silva S, Vico-Alonso C, Meyer L, Enderle D, Sanchez JA, Onteniente MDM, Noerholm M, Skog J, Rodríguez-Peralto JL, Ortiz-Romero PL, Peinado H. (2023). Improved Sensitivity in BRAFV600E Detection in Combined Tissue and Extracellular Vesicle-Based Liquid Biopsy in Melanoma. J Invest Dermatol 143, 1606-1610. Publicación CNIO.
Benito-Martín A, Jasiulionis MG, García-Silva S. (2023). Extracellular vesicles and melanoma: New perspectives on tumor microenvironment and metastasis. Front Cell Dev Biol 10, 1061982. Publicación CNIO.
González MI, Gallardo B, Cerón C, Aguilera-Jiménez E, Cortes-Canteli M, Peinado H, Desco M, Salinas B. (2023). Isolation of goat milk small extracellular vesicles by novel combined bio-physical methodology. Front. Bioeng. Biotechnol. 11, . Publicación CNIO.
Casadomé-Perales Á, Naya S, Fernández-Martínez E, Mille BG, Guerrero-Valero M, Peinado H, Guix FX, Dotti CG, Palomer E. (2023). Neuronal Prosurvival Role of Ceramide Synthase 2 by Olidogendrocyte-to-Neuron Extracellular Vesicle Transfer. Int J Mol Sci 24, 5986. Publicación CNIO.
Pericoli G, Galardi A, Paolini A, Petrilli LL, Pepe G, Palma A, Colletti M, Ferretti R, Giorda E, Levi Mortera S, Burford A, Carai A, Mastronuzzi A, Mackay A, Putignani L, Jones C, Pascucci L, Peinado H, Helmer-Citterich M, de Billy E, Masotti A, Locatelli F, Di Giannatale A, Vinci M. (2023). Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas. Cell Biosci 13, 207. Publicación CNIO.
Couto-Rodriguez A, Villaseñor A, Pablo-Torres C, Obeso D, Rey-Stolle MF, Peinado H, Bueno JL, Reaño-Martos M, Iglesias Cadarso A, Gomez-Casado C, Barbas C, Barber D, Escribese MM, Izquierdo E. (2023). Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24, 12714. Publicación CNIO.
Ruiz-Llorente L, Ruiz-Rodríguez MJ, Savini C, González-Muñoz T, Riveiro-Falkenbach E, Rodríguez-Peralto JL, Peinado H, Bernabeu C. (2023). Correlation Between Endoglin and Malignant Phenotype in Human Melanoma Cells: Analysis of hsa-mir-214 and hsa-mir-370 in Cells and Their Extracellular Vesicles. Adv Exp Med Biol 1408, 253-272. Publicación CNIO. Open Access
Burdiel M, Jiménez J, Rodríguez-Antolín C, García-Guede Á, Pernía O, Sastre-Perona A, Rosas-Alonso R, Colmenarejo J, Rodríguez-Jiménez C, Diestro MD, Martínez-Marín V, Higueras O, Cruz P, Losantos-García I, Peinado H, Vera O, de Castro J, Ibáñez de Cáceres I. (2023). MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer. Biomark Res 11, 94. Publicación CNIO.
González-Muñoz T, Kim A, Ratner N, Peinado H (2022). The Need for New Treatments Targeting MPNST: The Potential of Strategies Combining MEK Inhibitors with Antiangiogenic Agents. Clin Cancer Res 28, 3185-3195. Publicación CNIO.
Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, Collado-Diaz V, Halin C, Garcia-Silva S, Peinado H, Dieterich LC (2022). Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. J Extracell Vesicles 11, e12197. Publicación CNIO.
Benito-Martin A, Nogués L, Hergueta-Redondo M, Castellano-Sanz E, Garvin E, Cioffi M, Sola-Castrillo P, Buehring W, Ximénez-Embún P, Muñoz J, Matei I, Villanueva J, Peinado H. (2022). Mast cells impair melanoma cell homing and metastasis by inhibiting HMGA1 secretion. Immunology (in press). Publicación CNIO. Open Access
Carretero-González A, Hergueta-Redondo M, Sánchez-Redondo S, Ximénez-Embún P, Manso Sánchez L, Gil EC, Castellano D, de Velasco G, Peinado H. (2022). Characterization of plasma circulating small extracellular vesicles in patients with metastatic solid tumors and newly diagnosed brain metastasis. Oncoimmunology 11, 2067944. Publicación CNIO.
Almansa D, Peinado H, García-Rodríguez R, Casadomé-Perales Á, Dotti CG, Guix FX. (2022). Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro. Int J Mol Sci 23, 1371. Publicación CNIO.
Cardeñes B, Clares I, Bezos T, Toribio V, López-Martín S, Rocha A, Peinado H, Yáñez-Mó M, Cabañas C. (2022). ALCAM/CD166 Is Involved in the Binding and Uptake of Cancer-Derived Extracellular Vesicles. Int J Mol Sci 23, 5753. Publicación CNIO.
Verweij FJ, Balaj L, Boulanger CM, Carter DRF, Compeer EB, D'Angelo G, El Andaloussi S, Goetz JG, Gross JC, Hyenne V, Krämer-Albers EM, Lai CP, Loyer X, Marki A, Momma S, Nolte-'t Hoen ENM, Pegtel DM, Peinado H, Raposo G, Rilla K, Tahara H, Théry C, van Royen ME, Vandenbroucke RE, Wehman AM, Witwer K, Wu Z, Wubbolts R, van Niel G. (2021). The power of imaging to understand extracellular vesicle biology in vivo. Nat Methods 18, 1013-1026. Publicación CNIO.
Olmeda D, Cerezo-Wallis D, Castellano-Sanz E, García-Silva S, Peinado H, Soengas MS (2021). Physiological models for in vivo imaging and targeting the lymphatic system: Nanoparticles and extracellular vesicles. Adv Drug Deliver Rev 175, 113833. Publicación CNIO.
Santos-Coquillat A, González MI, Clemente-Moragón A, González-Arjona M, Albaladejo-García V, Peinado H, Muñoz J, Ximénez Embún P, Ibañez B, Oliver E, Desco M, Salinas B. (2021). Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging. Small 2, e2105421. Publicación CNIO.
Saltari A, Dzung A, Quadri M, Tiso N, Facchinello N, Hernández-Barranco A, Garcia-Silva S, Nogués L, Stoffel CI, Cheng PF, Turko P, Eichhoff OM, Truzzi F, Marconi A, Pincelli C, Peinado H, Dummer R, Levesque MP (2021). Specific activation of the CD271 intracellular domain in combination with chemotherapy or targeted therapy inhibits melanoma progression.. Cancer Res 81, 6044-6057. Publicación CNIO.
Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M (2021). CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells. J Extracell Vesicles 10, e12082. Publicación CNIO.
Villasante A, Godier-Furnemont A, Hernandez-Barranco A, Coq JL, Boskovic J, Peinado H, Mora J, Samitier J, Vunjak-Novakovic G (2021). Horizontal transfer of the stemness-related markers EZH2 and GLI1 by neuroblastoma-derived extracellular vesicles in stromal cells. Transl Res 237, 82-97. Publicación CNIO.
García-Silva S, Gallardo M, Peinado H (2021). DNA-Loaded Extracellular Vesicles in Liquid Biopsy: Tiny Players With Big Potential?. Front Cell Dev Biol 8, 622579. Publicación CNIO.
Hernández-Barranco A, Nogués L, Peinado H (2021). Could Extracellular Vesicles Contribute to Generation or Awakening of «Sleepy» Metastatic Niches?. Front Cell Dev Biol 9, 625221. Publicación CNIO.
Palacios-Ferrer JL, García-Ortega MB, Gallardo-Gómez M, García MÁ, Díaz C, Boulaiz H, Valdivia J, Jurado JM, Almazan-Fernandez FM, Arias-Santiago S, Amezcua V, Peinado H, Vicente F, Pérez Del Palacio J, Marchal JA (2021). Metabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanoma. Mol Oncol 15, 407-428. Publicación CNIO.
Rodríguez-Cobos J, Viñal D, Poves C, Fernández-Aceñero MJ, Peinado H, Pastor-Morate D, Prieto MI, Barderas R, Rodríguez-Salas N, Domínguez G (2021). DeltaNp73, TAp73 and Delta133p53 extracellular vesicle cargo as early diagnosis markers in colorectal cancer. Cancers 13, 2240. Publicación CNIO.
Royo F, Azkargorta M, Lavin JL, Clos-Garcia M, Cortazar AR, Gonzalez-Lopez M, Barcena L, Del Portillo HA, Yáñez-Mó M, Marcilla A, Borras FE, Peinado H, Guerrero I, Váles-Gómez M, Cereijo U, Sardon T, Aransay AM, Elortza F, Falcon-Perez JM (2021). Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules. Front Cell Dev Biol 8, 613583. Publicación CNIO.
Senís E et al. (incl. Peinado H). (2021). TUNAR lncRNA Encodes a Microprotein that Regulates Neural Differentiation and Neurite Formation by Modulating Calcium. Front Cell Dev Biol 9, 747667. Publicación CNIO.
Amor López A, Mazariegos MS, Capuano A, Ximénez-Embún P, Hergueta-Redondo M, Recio JÁ, Muñoz E, Al-Shahrour F, Muñoz J, Megías D, Doliana R, Spessotto P, Peinado H (2021). Inactivation of EMILIN-1 by Proteolysis and Secretion in Small Extracellular Vesicles Favors Melanoma Progression and Metastasis. Int J Mol Sci 22, 7406. Publicación CNIO.
González Muñoz T, Amaral AT, Puerto-Camacho P, Peinado H, de Álava E (2021). Endoglin in the Spotlight to Treat Cancer. Int J Mol Sci 22, 3186. Publicación CNIO.
Zhou J, Flores-Bellver M, Pan J, Benito-Martin A, Shi C, Onwumere O, Mighty J, Qian J, Zhong X, Hogue T, Amponsah-Antwi B, Einbond L, Gharbaran R, Wu H, Chen BJ, Zheng Z, Tchaikovskaya T, Zhang X, Peinado H, Canto-Soler MV, Redenti S (2021). Human retinal organoids release extracellular vesicles that regulate gene expression in target human retinal progenitor cells. Sci Rep 11, 21128. Publicación CNIO.
García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Cieckiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H (2021). Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism. Nat Cancer 2, 1387-1405. Publicación CNIO.
García-Silva S, Ximénez-Embún P, Muñoz J, Peinado H (2021). Postlymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients. Methods Mol Biol 2265, 345-359. Publicación CNIO.
González MI, González-Arjona M, Santos-Coquillat A, Vaquero J, Vázquez-Ogando E, de Molina A, Peinado H, Desco M, Salinas B (2021). Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines 9, 81. Publicación CNIO.
Guix FX, Capitán AM, Casadomé-Perales Á, Palomares-Pérez I, López Del Castillo I, Miguel V, Goedeke L, Martín MG, Lamas S, Peinado H, Fernández-Hernando C, Dotti CG. (2021). Increased exosome secretion in neurons aging in vitro by NPC1-mediated endosomal cholesterol buildup. Life Sci Alliance 4, e202101055. Publicación CNIO.
Hoshino A, Kim HS, Bojmar L, Gyan KE, Cioffi M, Hernandez J, Zambirinis CP, Rodrigues G, Molina H, Heissel S, Mark MT, Steiner L, Benito-Martin A, Lucotti S, Di Giannatale A, Offer K, Nakajima M, Williams C, Nogués L, Pelissier Vatter FA, Hashimoto A, Davies AE, Freitas D, Kenific CM, Ararso Y, Buehring W, Lauritzen P, Ogitani Y, Sugiura K, Takahashi N, Aleckovic M, Bailey KA, Jolissant JS, Wang H, Harris A, Schaeffer LM, García-Santos G, Posner Z, Balachandran VP, Khakoo Y, Raju GP, Scherz A, Sagi I, Scherz-Shouval R, Yarden Y, Oren M, Malladi M, Petriccione M, De Braganca KC, Donzelli M, Fischer C, Vitolano S, Wright GP, Ganshaw L, Marrano M, Ahmed A, DeStefano J, Danzer E, Roehrl MHA, Lacayo NJ, Vincent TC, Weiser MR, Brady MS, Meyers PA, Wexler LH, Ambati SR, Chou AJ, Slotkin EK, Modak S, Roberts SS, Basu EM, Diolaiti D, Krantz BA, Cardoso F, Simpson AL, Berger M, Rudin CM, Simeone DM, Jain M, Ghajar CM, Batra SK, Stanger BZ, Bui J, Brown KA, Rajasekhar VK, Healey JH, de Sousa M, Kramer K, Sheth S, Baisch J, Pascual V, Heaton TE, La Quaglia MP, Pisapia DJ, Schwartz R, Zhang H, Liu Y, Shukla A, Blavier L, DeClerck YA, LaBarge M, Bissell MJ, Caffrey TC, Grandgenett PM, Hollingsworth MA, Bromberg J, Costa-Silva B, Peinado H, Kang Y, Garcia BA, O'Reilly EM, Kelsen D, Trippett TM, Jones DR, Matei IR, Jarnagin WR, Lyden D (2020). Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. Cell 182, 1044-1061. Publicación CNIO.
Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S (2020). ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells. Nat Commun 11, 4261. Publicación CNIO. Open Access
Martinez-Lage M, Torres-Ruiz R, Puig-Serra P, Moreno-Gaona P, Martin MC, Moya FJ, Quintana-Bustamante O, Garcia-Silva S, Carcaboso AM, Petazzi P, Bueno C, Mora J, Peinado H, Segovia JC, Menendez P, Rodriguez-Perales S (2020). In vivo CRISPR/Cas9 targeting of fusion oncogenes for selective elimination of cancer cells. Nat Commun 11, 5060. Publicación CNIO. Open Access
Toda H, Diaz-Varela M, Segui-Barber J, Roobsoong W, Baro B, Garcia-Silva S, Galiano A, Gualdrón-López M, Almeida ACG, Brito MAM, de Melo GC, Aparici-Herraiz I, Castro-Cavadía C, Monteiro WM, Borràs E, Sabidó E, Almeida IC, Chojnacki J, Martinez-Picado J, Calvo M, Armengol P, Carmona-Fonseca J, Yasnot MF, Lauzurica R, Marcilla A, Peinado H, Galinski MR, Lacerda MVG, Sattabongkot J, Fernandez-Becerra C, Del Portillo HA (2020). Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence. Nat Commun 11, 2761. Publicación CNIO. Open Access
Colletti M, Tomao L, Galardi A, Paolini A, Di Paolo V, De Stefanis C, Mascio P, Nazio F, Petrini S, Castellano A, Russo I, Caruso R, Piga S, De Vito R, Pascucci L, Peinado H, Masotti A, Locatelli F, Di Giannatale A (2020). Neuroblastoma-secreted exosomes carrying miR-375 promote osteogenic differentiation of bone-marrow mesenchymal stromal cells. J Extracell Vesicles 9, 1774144. Publicación CNIO.
Hergueta-Redondo M, Peinado H. (2020). The influence of secreted factors and extracellular vesicles in ovarian cancer metastasis. Ejc Suppl 15, 38-48. Publicación CNIO.
Galardi A, Colletti M, Lavarello C, Di Paolo V, Mascio P, Russo I, Cozza R, Romanzo A, Valente P, De Vito R, Pascucci L, Peinado H, Carcaboso AM, Petretto A, Locatelli F, Di Giannatale A. (2020). Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding. Cancers 12, E1555. Publicación CNIO. Open Access
Palacios-Ferrer JL, García-Ortega MB, Gallardo-Gómez M, García MÁ, Díaz C, Boulaiz H, Valdivia J, Jurado JM, Almazan-Fernandez FM, Arias-Santiago S, Amezcua V, Peinado H, Vicente F, Pérez Del Palacio J, Marchal JA. (2020). Metabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanoma. Mol Oncol 15, 407-428. Publicación CNIO. Open Access
Ramón Y Cajal S, Sancho P, Soucek L, Peinado H, Abad M, Valiente M, Efeyan A, Pardo J, Quesada V, Jimeno J, Duque PM, Antón A, Varela I, Schuhmacher AJ (2020). A spotlight on cancer researchers in Spain: new paradigms and disruptive ideas. Clin Transl Oncol 22, 798-801. Publicación CNIO.
Ma W, Gil HJ, Escobedo N, Benito-Martín A, Ximénez-Embún P, Muñoz J, Peinado H, Rockson SG, Oliver G (2020). Platelet factor 4 is a biomarker for lymphatic-promoted disorders.. JCI Insight 5, e135109. Publicación CNIO.