Our laboratory is interested in analysing the main mechanisms involved in tumour-microenvironment communication during metastasis. Extracellular vesicles (EVs) and soluble factors are key players during this process. Tumour cells benefit from the secretion of these factors partly by influencing the behaviour of neighbouring cells in the tumour microenvironment. We are exploring the role of secreted factors and vesicles in metastasis using different approaches: 1) decoding the messages packaged in tumour-derived exosomes during melanoma metastasis, 2) defining novel factors involved in the crosstalk between adipose tissue and tumour, and 3) testing new therapies to prevent metastasis in rare diseases such as neurofibromatosis.
Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation.(2018) Zhang H, Freitas D, Kim HS, Fabijanic K, Li Z, Chen H, Mark MT, Molina H, Martin AB, Bojmar L, Fang J, Rampersaud S, Hoshino A, Matei I, Kenific CM, Nakajima M, Mutvei AP, Sansone P, Buehring W, Wang H, Jimenez JP, Cohen-Gould L, Paknejad N, Brendel M, Manova-Todorova K, Magalhães A, Ferreira JA, Osório H, Silva AM, Massey A, Cubillos-Ruiz JR, Galletti G, Giannakakou P, Cuervo AM, Blenis J, Schwartz R, Brady MS, Peinado H, Bromberg J, Matsui H, Reis CA, Lyden D. Nat Cell Biol 20, 332-343.
Neutrophils instruct homeostatic and pathological states in naive tissues.(2018) Casanova-Acebes M, Nicolás-Ávila JA, Li JL, García-Silva S, Balachander A, Rubio-Ponce A, Weiss LA, Adrover JM, Burrows K, A-González N, Ballesteros I, Devi S, Quintana JA, Crainiciuc G, Leiva M, Gunzer M, Weber C, Nagasawa T, Soehnlein O, Merad M, Mortha A, Ng LG, Peinado H, Hidalgo A. J Exp Med 215, 2778-2795.
Pre-metastatic niches: organ-specific homes for metastases.(2017) Peinado H, Zhang H, Matei IR, Costa-Silva B, Hoshino A, Rodrigues G, Psaila B, Kaplan RN, Bromberg JF, Kang Y, Bissell MJ, Cox TR, Giaccia AJ, Erler JT, Hiratsuka S, Ghajar CM, Lyden D Nat Rev Cancer 17, 302-317.
Phagocytosis imprints heterogeneity in tissue-resident macrophages(2017) A Gonzalez N, Quintana JA, García-Silva S, Mazariegos M, González de la Aleja A, Nicolás-Ávila JA, Walter W, Adrover JM, Crainiciuc G, Kuchroo VK, Rothlin CV, Peinado H, Castrillo A, Ricote M, Hidalgo A J Exp Med 214, 1281-1296.
Double-stranded DNA in exosomes: a novel biomarker in cancer detection.(2014) Thakur BK, Zhang H, Becker A, Matei I, Huang Y, Costa-Silva B, Zheng Y, Hoshino A, Brazier H, Xiang J, Williams C, Rodriguez-Barrueco R, Silva JM, Zhang W, Hearn S, Elemento O, Paknejad N, Manova-Todorova K, Welte K, Bromberg J, Peinado H, Lyden D Cell Res 24, 766-769.
The perivascular niche regulates breast tumour dormancy.(2013) Ghajar CM, Peinado H, Mori H, Matei IR, Evason KJ, Brazier H, Almeida D, Koller A, Hajjar KA, Stainier DY, Chen EI, Lyden D, Bissell MJ. Nat Cell Biol 15, 807-817.
