Grupo de Microambiente y Metástasis

Hector Peinado Jefe de Grupo
T +34 (+34) 917 328 000 (Ext 3520)
hpeinado@cnio.es

Investigadores

Susana García

Becarios Post-doctorales

Marta Hergueta
Laura Nogués

Becarios Pre-Doctorales

María Elena Castellano
María Teresa González
Alberto Hernández

Técnicos de Laboratorio

Irene Fernández
Sara Sánchez-Redondo
Vanesa Santos

Our laboratory aims to understand how the microenvironment impacts metastatic behaviour. Most of the efforts in the past focused on defining intrinsic factors involved in tumour progression and metastasis. However, our interpretation of tumour evolution and metastasis has shifted and currently it is widely accepted that extrinsic factors are actively involved in cancer progression. To tackle this question, our laboratory uses melanoma models to define the role of secreted exosomes in pre-metastatic niche formation and metastasis in the lymph node. In addition, we are exploring the influence of obesity on the metastatic behaviour of melanoma and breast cancer. Finally, we are using novel therapeutic approaches to prevent metastasis in melanoma, lymphoma and malignant peripheral nerve sheath tumour metastasis by targeting both intrinsic and extrinsic factors.

Publicaciones

García-Silva S, Ximénez-Embún P, Muñoz J, Peinado H (2021). Postlymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients. Methods Mol Biol 2265, 345-359. Publicación CNIO.
González Muñoz T, Amaral AT, Puerto-Camacho P, Peinado H, de Álava E (2021). Endoglin in the Spotlight to Treat Cancer. Int J Mol Sci 22, 3186. Publicación CNIO.
González MI, González-Arjona M, Santos-Coquillat A, Vaquero J, Vázquez-Ogando E, de Molina A, Peinado H, Desco M, Salinas B (2021). Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines 9, 81. Publicación CNIO.
Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M (2021). CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells. J Extracell Vesicles 10, e12082. Publicación CNIO.
Hernández-Barranco A, Nogués L, Peinado H (2021). Could Extracellular Vesicles Contribute to Generation or Awakening of «Sleepy» Metastatic Niches?. Front Cell Dev Biol 9, 625221. Publicación CNIO.
García-Silva S, Gallardo M, Peinado H (2021). DNA-Loaded Extracellular Vesicles in Liquid Biopsy: Tiny Players With Big Potential?. Front Cell Dev Biol 8, 622579. Publicación CNIO.
Royo F, Azkargorta M, Lavin JL, Clos-Garcia M, Cortazar AR, Gonzalez-Lopez M, Barcena L, Del Portillo HA, Yáñez-Mó M, Marcilla A, Borras FE, Peinado H, Guerrero I, Váles-Gómez M, Cereijo U, Sardon T, Aransay AM, Elortza F, Falcon-Perez JM (2021). Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules. Front Cell Dev Biol 8, 613583. Publicación CNIO.
Hoshino A, Kim HS, Bojmar L, Gyan KE, Cioffi M, Hernandez J, Zambirinis CP, Rodrigues G, Molina H, Heissel S, Mark MT, Steiner L, Benito-Martin A, Lucotti S, Di Giannatale A, Offer K, Nakajima M, Williams C, Nogués L, Pelissier Vatter FA, Hashimoto A, Davies AE, Freitas D, Kenific CM, Ararso Y, Buehring W, Lauritzen P, Ogitani Y, Sugiura K, Takahashi N, Aleckovic M, Bailey KA, Jolissant JS, Wang H, Harris A, Schaeffer LM, García-Santos G, Posner Z, Balachandran VP, Khakoo Y, Raju GP, Scherz A, Sagi I, Scherz-Shouval R, Yarden Y, Oren M, Malladi M, Petriccione M, De Braganca KC, Donzelli M, Fischer C, Vitolano S, Wright GP, Ganshaw L, Marrano M, Ahmed A, DeStefano J, Danzer E, Roehrl MHA, Lacayo NJ, Vincent TC, Weiser MR, Brady MS, Meyers PA, Wexler LH, Ambati SR, Chou AJ, Slotkin EK, Modak S, Roberts SS, Basu EM, Diolaiti D, Krantz BA, Cardoso F, Simpson AL, Berger M, Rudin CM, Simeone DM, Jain M, Ghajar CM, Batra SK, Stanger BZ, Bui J, Brown KA, Rajasekhar VK, Healey JH, de Sousa M, Kramer K, Sheth S, Baisch J, Pascual V, Heaton TE, La Quaglia MP, Pisapia DJ, Schwartz R, Zhang H, Liu Y, Shukla A, Blavier L, DeClerck YA, LaBarge M, Bissell MJ, Caffrey TC, Grandgenett PM, Hollingsworth MA, Bromberg J, Costa-Silva B, Peinado H, Kang Y, Garcia BA, O'Reilly EM, Kelsen D, Trippett TM, Jones DR, Matei IR, Jarnagin WR, Lyden D (2020). Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. Cell 182, 1044-1061. Publicación CNIO.
Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S (2020). ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells. Nat Commun 11, 4261. Publicación CNIO. Open Access
Toda H, Diaz-Varela M, Segui-Barber J, Roobsoong W, Baro B, Garcia-Silva S, Galiano A, Gualdrón-López M, Almeida ACG, Brito MAM, de Melo GC, Aparici-Herraiz I, Castro-Cavadía C, Monteiro WM, Borràs E, Sabidó E, Almeida IC, Chojnacki J, Martinez-Picado J, Calvo M, Armengol P, Carmona-Fonseca J, Yasnot MF, Lauzurica R, Marcilla A, Peinado H, Galinski MR, Lacerda MVG, Sattabongkot J, Fernandez-Becerra C, Del Portillo HA (2020). Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence. Nat Commun 11, 2761. Publicación CNIO. Open Access
Galardi A, Colletti M, Lavarello C, Di Paolo V, Mascio P, Russo I, Cozza R, Romanzo A, Valente P, De Vito R, Pascucci L, Peinado H, Carcaboso AM, Petretto A, Locatelli F, Di Giannatale A. (2020). Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding. Cancers 12, E1555. Publicación CNIO. Open Access
Ramón Y Cajal S, Sancho P, Soucek L, Peinado H, Abad M, Valiente M, Efeyan A, Pardo J, Quesada V, Jimeno J, Duque PM, Antón A, Varela I, Schuhmacher AJ (2020). A spotlight on cancer researchers in Spain: new paradigms and disruptive ideas. Clin Transl Oncol 22, 798-801. Publicación CNIO.
Ma W, Gil HJ, Escobedo N, Benito-Martín A, Ximénez-Embún P, Muñoz J, Peinado H, Rockson SG, Oliver G (2020). Platelet factor 4 is a biomarker for lymphatic-promoted disorders.. JCI Insight 5, e135109. Publicación CNIO.
Martinez-Lage M, Torres-Ruiz R, Puig-Serra P, Moreno-Gaona P, Martin MC, Moya FJ, Quintana-Bustamante O, Garcia-Silva S, Carcaboso AM, Petazzi P, Bueno C, Mora J, Peinado H, Segovia JC, Menendez P, Rodriguez-Perales S (2020). In vivo CRISPR/Cas9 targeting of fusion oncogenes for selective elimination of cancer cells. Nat Commun 11, 5060. Publicación CNIO. Open Access
Colletti M, Tomao L, Galardi A, Paolini A, Di Paolo V, De Stefanis C, Mascio P, Nazio F, Petrini S, Castellano A, Russo I, Caruso R, Piga S, De Vito R, Pascucci L, Peinado H, Masotti A, Locatelli F, Di Giannatale A (2020). Neuroblastoma-secreted exosomes carrying miR-375 promote osteogenic differentiation of bone-marrow mesenchymal stromal cells. J Extracell Vesicles 9, 1774144. Publicación CNIO.
Hergueta-Redondo M, Peinado H. (2020). The influence of secreted factors and extracellular vesicles in ovarian cancer metastasis. Ejc Suppl 15, 38-48. Publicación CNIO.
Palacios-Ferrer JL, García-Ortega MB, Gallardo-Gómez M, García MÁ, Díaz C, Boulaiz H, Valdivia J, Jurado JM, Almazan-Fernandez FM, Arias-Santiago S, Amezcua V, Peinado H, Vicente F, Pérez Del Palacio J, Marchal JA. (2020). Metabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanoma. Mol Oncol 15, 407-428. Publicación CNIO. Open Access
García-Silva S, Benito-Martín A, Sánchez-Redondo S, Hernández-Barranco A, Ximénez-Embún P, Nogués L, Mazariegos MS, Brinkmann K, Amor López A, Meyer L, Rodríguez C, García-Martín C, Boskovic J, Letón R, Montero C, Robledo M, Santambrogio L, Sue Brady M, Szumera-Cieckiewicz A, Kalinowska I, Skog J, Noerholm M, Muñoz J, Ortiz-Romero PL, Ruano Y, Rodríguez-Peralto JL, Rutkowski P, Peinado H (2019). Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF V600E mutation. J Exp Med 216, 1061-1070. Publicación CNIO.
Borghesan M, Fafián-Labora J, Eleftheriadou O, Carpintero-Fernández P, Paez-Ribes M, Vizcay-Barrena G, Swisa A, Kolodkin-Gal D, Ximénez-Embún P, Lowe R, Martín-Martín B, Peinado H, Muñoz J, Fleck RA, Dor Y, Ben-Porath I, Vossenkamper A, Muñoz-Espin D, O'Loghlen A (2019). Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3.. Cell Reports 27, 3956-3971. Publicación CNIO. Open Access
Capuano A, Pivetta E, Sartori G, Bosisio G, Favero A, Cover E, Andreuzzi E, Colombatti A, Cannizzaro R, Scanziani E, Minoli L, Bucciotti F, Amor Lopez AI, Gaspardo K, Doliana R, Mongiat M, Spessotto P (2019). Abrogation of EMILIN1-ß1 integrin interaction promotes experimental colitis and colon carcinogenesis. Matrix Biol 83, 97-115. Publicación CNIO.
Colletti M, Paolini A, Galardi A, Di Paolo V, Pascucci L, Russo I, De Angelis B, Peinado H, De Vito R, Milano GM, Locatelli F, Masotti A, Di Giannatale A (2019). Expression profiles of exosomal miRNAs isolated from plasma of patients with desmoplastic small round cell tumor. Epigenomics 11, 489-500. Publicación CNIO.
Gabandé-Rodríguez E, Pérez-Cañamás A, Soto-Huelin B, Mitroi DN, Sánchez-Redondo S, Martínez-Sáez E, Venero C, Peinado H, Ledesma MD (2019). Lipid-induced lysosomal damage after demyelination corrupts microglia protective function in lysosomal storage disorders. EMBO J 38, e99553. Publicación CNIO.
Hyenne V, Ghoroghi S, Collot M, Bons J, Follain G, Harlepp S, Mary B, Bauer J, Mercier L, Busnelli I, Lefebvre O, Fekonja N, Garcia-Leon MJ, Machado P, Delalande F, López AA, Silva SG, Verweij FJ, van Niel G, Djouad F, Peinado H, Carapito C, Klymchenko AS, Goetz JG (2019). Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo. Dev Cell 48, 554-572. Publicación CNIO.
Rodrigues G, Hoshino A, Kenific CM, Matei IR, Steiner L, Freitas D, Kim HS, Oxley PR, Scandariato I, Casanova-Salas I, Dai J, Badwe CR, Gril B, Tešic Mark M, Dill BD, Molina H, Zhang H, Benito-Martin A, Bojmar L, Ararso Y, Offer K, LaPlant Q, Buehring W, Wang H, Jiang X, Lu TM, Liu Y, Sabari JK, Shin SJ, Narula N, Ginter PS, Rajasekhar VK, Healey JH, Meylan E, Costa-Silva B, Wang SE, Rafii S, Altorki NK, Rudin CM, Jones DR, Steeg PS, Peinado H, Ghajar CM, Bromberg J, de Sousa M, Pisapia D, Lyden D (2019). Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis.. Nat Cell Biol 21, 1403-1412. Publicación CNIO.
Zhang H, Freitas D, Kim HS, Fabijanic K, Li Z, Chen H, Mark MT, Molina H, Martin AB, Bojmar L, Fang J, Rampersaud S, Hoshino A, Matei I, Kenific CM, Nakajima M, Mutvei AP, Sansone P, Buehring W, Wang H, Jimenez JP, Cohen-Gould L, Paknejad N, Brendel M, Manova-Todorova K, Magalhães A, Ferreira JA, Osório H, Silva AM, Massey A, Cubillos-Ruiz JR, Galletti G, Giannakakou P, Cuervo AM, Blenis J, Schwartz R, Brady MS, Peinado H, Bromberg J, Matsui H, Reis CA, Lyden D (2018). Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nat Cell Biol 20, 332-343. Publicación CNIO.
Casanova-Acebes M, Nicolás-Ávila JA, Li JL, García-Silva S, Balachander A, Rubio-Ponce A, Weiss LA, Adrover JM, Burrows K, A-González N, Ballesteros I, Devi S, Quintana JA, Crainiciuc G, Leiva M, Gunzer M, Weber C, Nagasawa T, Soehnlein O, Merad M, Mortha A, Ng LG, Peinado H, Hidalgo A (2018). Neutrophils instruct homeostatic and pathological states in naive tissues. J Exp Med 215, 2778-2795. Publicación CNIO.
Nogués L, Benito-Martin A, Hergueta-Redondo M, Peinado H (2018). The influence of tumour-derived extracellular vesicles on local and distal metastatic dissemination. Mol Aspects Med 60, 15-26. Publicación CNIO.
Di Paolo V, Russo I, Boldrini R, Ravà L, Pezzullo M, Benedetti MC, Galardi A, Colletti M, Rota R, Orlando D, Crocoli A, Peinado H, Milano GM, Di Giannatale A (2018). Evaluation of Endoglin (CD105) expression in pediatric rhabdomyosarcoma. Bmc Cancer 18, 31. Publicación CNIO. Open Access
García-Rodríguez A, de la Casa M, Peinado H, Gosálvez J, Roy R (2018). Human prostasomes from normozoospermic and non-normozoospermic men show a differential protein expression pattern. Andrology 6, 585-596. Publicación CNIO.
Robado de Lope L, Alcíbar OL, Amor López A, Hergueta-Redondo M, Peinado H (2018). Tumour-adipose tissue crosstalk: fuelling tumour metastasis by extracellular vesicles. Philos Trans R Soc Lond B Biol Sci. 373, pii: 20160485. Publicación CNIO.
Santamaría PG, Floristán A, Fontanals-Cirera B, Vázquez-Naharro A, Santos V, Morales S, Yuste L, Peinado H, García-Gómez A, Portillo F, Hernando E, Cano A (2018). Lysyl oxidase-like 3 is required for melanoma cell survival by maintaining genomic stability. Cell Death Differ 25, 935-950. Publicación CNIO.
Peinado H, Zhang H, Matei IR, Costa-Silva B, Hoshino A, Rodrigues G, Psaila B, Kaplan RN, Bromberg JF, Kang Y, Bissell MJ, Cox TR, Giaccia AJ, Erler JT, Hiratsuka S, Ghajar CM, Lyden D (2017). Pre-metastatic niches: organ-specific homes for metastases.. Nat Rev Cancer 17, 302-317. Publicación CNIO.
Huang Y, Rajappa P, Hu W, Hoffman C, Cisse B, Kim JH, Gorge E, Yanowitch R, Cope W, Vartanian E, Xu R, Zhang T, Pisapia D, Xiang J, Huse J, Matei I, Peinado H, Bromberg J, Holland E, Ding BS, Rafii S, Lyden D, Greenfield J (2017). A proangiogenic signaling axis in myeloid cells promotes malignant progression of glioma. J Clin Invest 127, 1826-1838. Publicación CNIO.
A Gonzalez N, Quintana JA, García-Silva S, Mazariegos M, González de la Aleja A, Nicolás-Ávila JA, Walter W, Adrover JM, Crainiciuc G, Kuchroo VK, Rothlin CV, Peinado H, Castrillo A, Ricote M, Hidalgo A (2017). Phagocytosis imprints heterogeneity in tissue-resident macrophages. J Exp Med 214, 1281-1296. Publicación CNIO.
Colletti M, Petretto A, Galardi A, Di Paolo V, Tomao L, Lavarello C, Inglese E, Bruschi M, Lopez AA, Pascucci L, Geoerger B, Peinado H, Locatelli F, Di Giannatale A (2017). Proteomic Analysis of Neuroblastoma-Derived Exosomes: New Insights into a Metastatic Signature.. Proteomics 17, 23-24. Publicación CNIO.
Pathan M1, Keerthikumar S1, Chisanga D2, Alessandro R3, Ang CS4, Askenase P5, Batagov AO6, Benito-Martin A7, Camussi G8, Clayton A9, Collino F8,10, Di Vizio D11, Falcon-Perez JM12, Fonseca P13, Fonseka P1, Fontana S3, Gho YS14, Hendrix A15, Hoen EN16, Iraci N17,18, Kastaniegaard K19, Kislinger T20, Kowal J21, Kurochkin IV6, Leonardi T18,22, Liang Y23, Llorente A24,25, Lunavat TR26, Maji S27, Monteleone F3, Øverbye A24,25, Panaretakis T13, Patel T27, Peinado H7,28, Pluchino S18, Principe S20, Ronquist G29, Royo F12, Sahoo S23, Spinelli C11, Stensballe A19, Théry C21, van Herwijnen MJC16, Wauben M16, Welton JL30, Zhao K1, Mathivanan S (2017). A novel community driven software for functional enrichment analysis of extracellular vesicles data. J Extracell Vesicles 6, 1321455. Publicación CNIO.
Becker A, Thakur BK, Weiss JM, Kim HS, Peinado H, Lyden D (2016). Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis.. Cancer Cell 30, 836-848. Publicación CNIO.
García-Silva S, Peinado H (2016). Melanosomes foster a tumour niche by activating CAFs.. Nat Cell Biol 18, 911-913. Publicación CNIO.
Zhou J,Ghoroghi S, Benito-Martin A, Wu H1, Unachukwu UJ, Einbond LS, Guariglia S, Peinado H, Redenti S (2016). Characterization of Induced Pluripotent Stem Cell Microvesicle Genesis, Morphology and Pluripotent Content. Sci Rep 6, 19743. Publicación CNIO. Open Access
Torrano V, Royo F, Peinado H, Loizaga-Iriarte A, Unda M, Falcón-Perez JM, Carracedo A. (2016). Vesicle-MaNiA: extracellular vesicles in liquid biopsy and cancer.. Curr Opin Pharmacol 29, 47-53. Publicación CNIO.
Veerappan A, Thompson M, Savage AR, Silverman ML, Chan WS, Sung B, Summers B, Montelione KC, Benedict P, Groh B, Vicencio AG, Peinado H, Worgall S, Silver RB (2016). Mast cells and exosomes in hyperoxia-induced neonatal lung disease.. Am J Physiol-Lung C 310, L1218-L1232. Publicación CNIO.