Our Group uses cryo-electron microscopy (cryoEM) to determine the 3D structure of large macromolecular complexes of relevance in cancer. Structural information, in combination with molecular and cell biology and biochemistry, is then used to propose how these molecules work and increase our understanding of the molecular basis of cancer. Most of our efforts are currently focused on 2 major areas of research: i) the study of chaperones essential for the activation of several macromolecular complexes relevant in cancer such as mTORC1; and ii) the study of complexes implicated in the repair of DNA damage and in genomic instability. In collaboration with other groups, we are also studying the structure and mechanisms of several amino acid transporters, as well as the mechanisms that regulate microtubule nucleation for the assembly of the mitotic spindle.
Investigadores Científicos
- Javier Coloma
- Ana I. Hernández
- Ángel Rivera
- Marina Serna
Becarios Post-doctorales
- Jaime Louro
- Ana González
- María I. Daudén
- Andrés López
- María Martínez
- Clara Reglero
Becarios Pre-Doctorales
- Carmen García
- Nayim González
- Álvaro López
- Juan Tasis
Publicaciones
- (2025). The yeast CST and Polα/primase complexes act in concert to ensure proper telomere maintenance and protection. Nucleic Acids Res 53, gkaf245. Publicación CNIO.
- (2025). The structure of the R2T complex reveals a different architecture from the related HSP90 cochaperone R2TP. Structure (in press). Publicación CNIO.
- (2024). Transition of human γ-tubulin ring complex into a closed conformation during microtubule nucleation. Science 383, 870-876. Publicación CNIO.
- (2024). Structure and mechanisms of transport of human Asc1/CD98hc amino acid transporter. Nat Commun 15, 2986. Publicación CNIO.
Open Access - (2024). CDK5RAP2 activates microtubule nucleator gTuRCby facilitating template formation and actin release. Dev Cell 59, 3175-3188. Publicación CNIO.
- (2024). Mechanism of allosteric inhibition of RUVBL1-RUVBL2 ATPase by the small molecule CB-6644. Cell Rep Phys Sci 5, 101982. Publicación CNIO.
- (2024). Maturation and Assembly of mTOR Complexes by the HSP90-R2TP-TTT Chaperone System: Molecular Insights and Mechanisms. Subcell Biochem 104, 459-483. Publicación CNIO.
- (2023). Molecular architecture and oligomerization of Candida glabrata Cdc13 underpin its telomeric DNA-binding and unfolding activity. Nucleic Acids Res 51, 668-686. Publicación CNIO. Open Access
- (2023). APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining. Cell Reports 42, 111917. Publicación CNIO. Open Access
- (2022). CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8. Nucleic Acids Res 50, 1128-1146. Publicación CNIO. Open Access
- (2022). Structural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus.. Nat Commun 13, 7062. Publicación CNIO. Open Access
- (2022). HATs meet structural biology. Curr Opin Struc Biol 74, 102389. Publicación CNIO.
- (2021). Type VII secretion systems: structure, functions and transport models. Nat Rev Microbiol 19, 567-584. Publicación CNIO.
- (2021). Long noncoding RNA NIHCOLE promotes ligation efficiency of DNA double-strand breaks in hepatocellular carcinoma.. Cancer Res 81, 4910-4925. Publicación CNIO.
- (2021). Structural basis for substrate specificity of heteromeric transporters of neutral amino acids.. Proc Natl Acad Sci USA 118, e2113573118. Publicación CNIO.
- (2021). Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone. Cell Reports 36, 109317. Publicación CNIO. Open Access
- (2021). The Bacterial Mucosal Immunotherapy MV130 Protects Against SARS-CoV-2 Infection and Improves COVID-19 Vaccines Immunogenicity. Front Inmmunol 12, 748103. Publicación CNIO. Open Access
- (2021). RUVBL1-RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Curr Opin Struc Biol 19, 78-85. Publicación CNIO.
- (2020). Assembly of the asymmetric human ?-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Sci Adv 6, eabe0894. Publicación CNIO. Open Access
- (2020). Structural basis of Focal Adhesion Kinase activation on lipid membranes.. EMBO J 39, e104743. Publicación CNIO. Open Access
- (2020). Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife 9, e63042. Publicación CNIO. Open Access
- (2020). RUVBL1–RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Curr Opin Struc Biol 67, 78-85. Publicación CNIO.
- (2020). Modeling of a 14 kDa RUVBL2-Binding Domain with Medium Resolution Cryo-EM Density.. J Chem Inf Model 60, 2541-2551. Publicación CNIO.
- (2020). RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes. Cells 9, E1139. Publicación CNIO. Open Access