Our Group uses cryo-electron microscopy (cryoEM) to determine the 3D structure of large macromolecular complexes of relevance in cancer at high resolution. Structural information, in combination with molecular and cell biology and biochemistry, is then used to propose how these molecules work and increase our understanding of the molecular basis of cancer. Most of our efforts are currently focused on 2 major areas of research: the study of chaperones essential for the activation of several macromolecular complexes relevant in cancer such as those formed by the mTOR kinase, and the study of complexes implicated in the repair of DNA double-strand breaks. In addition, and in collaboration with other groups, we are studying the structure and mechanisms of several amino acid transporters.

Investigadores
- Javier Coloma
- Ana I. Hernández
- María Ibarra
- Andrés López
- Ángel Rivera
- Marina Serna
Becarios Post-doctorales
- Ana González
- María Martínez
Becarios Pre-Doctorales
- Natalia Cuervo
- Carmen García
- Nayim González
Publicaciones
- (2023). Molecular architecture and oligomerization of Candida glabrata Cdc13 underpin its telomeric DNA-binding and unfolding activity. Nucleic Acids Res 51, 668-686. Publicación CNIO.
- (2023). APLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining. Cell Reports 42, 111917. Publicación CNIO.
- (2022). CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8. Nucleic Acids Res 50, 1128-1146. Publicación CNIO.
Open Access
- (2022). Structural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus.. Nat Commun 13, 7062. Publicación CNIO.
Open Access
- (2022). HATs meet structural biology. Curr Opin Struc Biol 74, 102389. Publicación CNIO.
- (2021). Type VII secretion systems: structure, functions and transport models. Nat Rev Microbiol 19, 567-584. Publicación CNIO.
- (2021). Long noncoding RNA NIHCOLE promotes ligation efficiency of DNA double-strand breaks in hepatocellular carcinoma.. Cancer Res 81, 4910-4925. Publicación CNIO.
- (2021). Structural basis for substrate specificity of heteromeric transporters of neutral amino acids.. Proc Natl Acad Sci USA 118, e2113573118. Publicación CNIO.
- (2021). Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone. Cell Reports 36, 109317. Publicación CNIO.
- (2021). The Bacterial Mucosal Immunotherapy MV130 Protects Against SARS-CoV-2 Infection and Improves COVID-19 Vaccines Immunogenicity. Front Inmmunol 12, 748103. Publicación CNIO.
- (2021). RUVBL1-RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Curr Opin Struc Biol 19, 78-85. Publicación CNIO.
- (2020). Assembly of the asymmetric human ?-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Sci Adv 6, eabe0894. Publicación CNIO.
- (2020). Structural basis of Focal Adhesion Kinase activation on lipid membranes.. EMBO J 39, e104743. Publicación CNIO.
- (2020). Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM. Elife 9, e63042. Publicación CNIO.
Open Access
- (2020). RUVBL1–RUVBL2 AAA-ATPase: a versatile scaffold for multiple complexes and functions. Curr Opin Struc Biol 67, 78-85. Publicación CNIO.
- (2020). Modeling of a 14 kDa RUVBL2-Binding Domain with Medium Resolution Cryo-EM Density.. J Chem Inf Model 60, 2541-2551. Publicación CNIO.
- (2020). RPAP3 C-Terminal Domain: A Conserved Domain for the Assembly of R2TP Co-Chaperone Complexes. Cells 9, E1139. Publicación CNIO.
- (2019). Architecture of the mycobacterial type VII secretion system. Nature 576, 321-325. Publicación CNIO.
- (2019). Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM. Sci Adv 5, eaaw1616. Publicación CNIO.
Open Access
- (2019). Recurrent Germline DLST Mutations in Individuals with Multiple Pheochromocytomas and Paragangliomas. Am J Hum Genet 104, 651-664. Publicación CNIO.
- (2019). Editorial: Technical Advances in Cryo-Electron Microscopy.. Front Mol Biosci 6, 72. Publicación CNIO.
Open Access
- (2019). Hands on Methods for High Resolution Cryo-Electron Microscopy Structures of Heterogeneous Macromolecular Complexes.. Front Mol Biosci 6, 33. Publicación CNIO.
Open Access
- (2018). RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex. Nat Commun 9, 1501. Publicación CNIO.
Open Access
- (2018). CryoEM reveals how the complement membrane attack complex ruptures lipid bilayers. Nat Commun 9, 5316. Publicación CNIO.
Open Access
- (2018). How novel structures inform understanding of complement function. Semin Immunopathol 40, 3-14. Publicación CNIO.
- (2018). Advances on the Structure of the R2TP/Prefoldin-like Complex. Adv Exp Med Biol 1106, 73-83. Publicación CNIO.