“Este contenido se encuentra unicamente en inglés”

Javier Muñoz Jefe de Unidad
T +34 (+34) 917 328 000 (Ext 3110)
jmunozpe@cnio.es

Proteins are the molecular effectors of cells and catalyse almost all biological processes. The levels of protein abundance, together with their modification states and interactions, adapt dynamically to external or internal (genetic) stimuli and thus define the cell’s functional state and determine its phenotype. Mass spectrometrybased proteomics is the most powerful tool to study the proteome, providing fundamental information of basic biology. In addition, recent improvements in sensitivity and throughput now allow the analysis of larger cohorts of samples including biopsies, thus fully integrating proteomics into the clinical research toolbox. All these efforts have led to the provision of new insights into the molecular mechanisms underlying cancer development as well as the identification of novel biomarkers.

Publicaciones

• In-Depth Proteomic Characterization of Classical and Non-Classical Monocyte Subsets.(2018)
  Segura V, Valero ML, Cantero L, Muñoz J, Zarzuela E, García F, Aloria K, Beaskoetxea J, Arizmendi JM, Navajas R, Paradela A, Díez P, Dégano RM, Fuentes M, Orfao A, Montero AG, Garin-Muga A, Corrales FJ, Pino MMSD
  Proteomes 6, E8-.
• In vivo phosphoproteomics reveals kinase activity profiles that predict treatment outcome in triple-negative breast cancer.(2018)
  Zagorac I, Fernandez-Gaitero S, Penning R, Post H, Bueno MJ, Mouron S, Manso L, Morente MM, Alonso S, Serra V, Muñoz J, Gómez-López G, Lopez-Acosta JF, Jimenez-Renard V, Gris-Oliver A, Al-Shahrour F, Piñeiro-Yañez E, Montoya-Suarez JL, Apala JV, Moreno-Torres A, Colomer R, Dopazo A, Heck AJR, Altelaar M, Quintela-Fandino M
  Nat Commun 9, 3501-.
• Thrombocytopenia-associated mutations in Ser/Thr kinase MASTL deregulate actin cytoskeleton dynamics in platelets(2018)
  Hurtado B, Trakala M, Ximénez-Embún P, El-Bakkali A, Partida D, Sanz-Castillo B, Álvarez-Fernández M, Maroto M, Sánchez-Martínez R, Martínez L, Muñoz J, García de Frutos P, Malumbres M
  J Clin Invest 128, 5351-5367.
• The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.(2018)
  Lynch CJ, Bernad R, Calvo I, Nóbrega-Pereira S, Ruiz S, Ibarz N, Martinez-Val A, Graña-Castro O, Gómez-López G, Andrés-León E, Espinosa Angarica V, Del Sol A, Ortega S, Fernandez-Capetillo O, Rojo E, Munoz J, Serrano M
  Cell Reports 22, 396-410.
• STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.(2018)
  Priego N, Zhu L, Monteiro C, Mulders M, Wasilewski D, Bindeman W, Doglio L, Martínez L, Martínez-Saez E, Cajal SRY, Megías D, Hernández-Encinas E, Blanco-Aparicio C, Martínez L, Zarzuela E, Muñoz J, Fustero-Torres C, Pineiro E, Hernández-Laín A, Bertero L, Poli V, Sánchez-Martínez M, Menendez JA, Soffietti R, Bosch-Barrera J, Valiente M
  Nat Med 24, 1024-1035.
• Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine.(2017)
  Olmeda D, Cerezo-Wallis D, Riveiro-Falkenbach E, Pennacchi PC, Contreras-Alcalde M, Ibarz N, Cifdaloz M, Catena X, Calvo TG, Cañón E, Alonso-Curbelo D, Suarez J, Osterloh L, Graña O, Mulero F, Megías D, Cañamero M, Martínez-Torrecuadrada JL, Mondal C, Di Martino J, Lora D, Martinez-Corral I, Bravo-Cordero JJ, Muñoz J, Puig S, Ortiz-Romero P, Rodriguez-Peralto JL, Ortega S, Soengas MS
  Nature 546, 676-680.
• mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.(2017)
  Zabala-Letona A, Arruabarrena-Aristorena A, Martín-Martín N, Fernandez-Ruiz S, Sutherland JD, Clasquin M, Tomas-Cortazar J, Jimenez J, Torres I, Quang P, Ximenez-Embun P, Bago R, Ugalde-Olano A, Loizaga-Iriarte A, Lacasa-Viscasillas I, Unda M, Torrano V, Cabrera D, van Liempd SM, Cendon Y, Castro E, Murray S, Revandkar A, Alimonti A, Zhang Y, Barnett A, Lein G, Pirman D, Cortazar AR, Arreal L, Prudkin L, Astobiza I, Valcarcel-Jimenez L, Zuñiga-García P, Fernandez-Dominguez I, Piva M, Caro-Maldonado A, Sánchez-Mosquera P, Castillo-Martín M, Serra V, Beraza N, Gentilella A, Thomas G, Azkargorta M, Elortza F, Farràs R, Olmos D, Efeyan A, Anguita J, Muñoz J, Falcón-Pérez JM, Barrio R, Macarulla T, Mato JM, Martinez-Chantar ML, Cordon-Cardo C, Aransay AM, Marks K, Baselga J, Tabernero J, Nuciforo P, Manning BD, Marjon K, Carracedo A
  Nature 547, 109-113.
• The molecular pathogenesis of the NUP98-HOXA9 fusion protein in acute myeloid leukemia.(2017)
  Rio-Machin A, Gómez-López G, Muñoz J, Garcia-Martinez F, Maiques-Diaz A, Alvarez S, Salgado RN, Shrestha M, Torres-Ruiz R, Haferlach C, Larráyoz MJ, Calasanz MJ, Fitzgibbon J, Cigudosa JC
  Leukemia 31, 2000-2005.
• Urea Artifacts Interfere with Immuno-Purification of Lysine Acetylation.(2017)
  Martinez-Val A, Garcia F, Ximénez-Embún P, Martínez Teresa-Calleja A, Ibarz N, Ruppen I, Munoz J
  J Proteome Res 16, 1061-1068.
• A multicentric study to evaluate the use of relative retention times in targeted proteomics.(2017)
  Vialas V, Colomé-Calls N, Abian J, Aloria K, Alvarez-Llamas G, Antúnez O, Arizmendi JM, Azkargorta M, Barceló-Batllori S, Barderas MG, Blanco F, Casal JI, Casas V, de la Torre C, Chicano-Gálvez E, Elortza F, Espadas G, Estanyol JM, Fernandez-Irigoyen J, Fernandez-Puente P, Fidalgo MJ, Fuentes M, Gay M, Gil C, Hainard A, Hernaez ML, Ibarrola N1, Kopylov AT, Lario A, Lopez JA, López-Lucendo M, Marcilla M, Marina-Ramírez A, Marko-Varga G, Martín L, Mora MI, Morato-López E, Muñoz J, Odena MA, de Oliveira E, Orera I, Ortea I, Pasquarello C, Ray KB, Rezeli M, Ruppen I, Sabidó E, Del Pino MM, Sancho J, Santamaría E, Vazquez J, Vilaseca M1 Vivanco F, Walters JJ, Zgoda VG, Corrales FJ, Canals F, Paradela A
  J Proteomics 152, 138-149.
• USP7 is a SUMO deubiquitinase essential for DNA replication.(2016)
  Lecona E, Rodriguez-Acebes S, Specks J, Lopez-Contreras AJ, Ruppen I, Murga M, Muñoz J, Mendez J, Fernandez-Capetillo O
  Nat Struct Mol Biol 23, 270-277.
• Targeting Tumor Mitochondrial Metabolism Overcomes Resistance to Antiangiogenics.(2016)
  Navarro P, Bueno MJ, Zagorac I, Mondejar T, Sanchez J, Mourón S, Muñoz J, Gómez-López G, Jimenez-Renard V, Mulero F, Chandel NS, Quintela-Fandino M
  Cell Reports 15, 2705-2718.
• On the Statistical Significance of Compressed Ratios in Isobaric Labeling: A Cross-Platform Comparison.(2016)
  Martinez-Val A, Garcia F, Ximénez-Embún P, Ibarz N, Zarzuela E, Ruppen I, Mohammed S, Munoz J
  J Proteome Res 15, 3029-3038.
• CD24 tracks divergent pluripotent states in mouse and human cells.(2015)
  Shakiba N, White CA, Lipsitz YY, Yachie-Kinoshita A, Tonge PD, Hussein SM, Puri MC, Elbaz J, Morrissey-Scoot J, Li M, Munoz J, Benevento M, Rogers IM, Hanna JH, Heck AJ, Wollscheid B, Nagy A, Zandstra PW
  Nat Communications 6, 7329-.
• Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells(2015)
  The I, Ruijtenberg S, Bouchet BP, Cristobal A, Prinsen MB, van Mourik T, Koreth J, Xu H, Heck AJ, Akhmanova A, Cuppen E, Boxem M, Muñoz J, van den Heuvel S
  Nat Communications 6, 5906-.
• Terminal epidermal differentiation is regulated by the interaction of Fra-2/AP-1 with Ezh2 and ERK1/2.(2015)
  Wurm S, Zhang J, Guinea-Viniegra J, García F, Muñoz J, Bakiri L, Ezhkova E, Wagner EF
  Genes Dev 29, 144-156.
• DAZL regulates Tet1 translation in murine embryonic stem cells.(2015)
  Welling M, Chen HH, Muñoz J, Musheev MU, Kester L, Junker JP, Mischerikow N, Arbab M, Kuijk E, Silberstein L, Kharchenko PV, Geens M, Niehrs C, van de Velde H, van Oudenaarden A, Heck AJ, Geijsen N
  EMBO Rep 16, 791-802.
• DAZL Limits Pluripotency, Differentiation, and Apoptosis in Developing Primordial Germ Cells.(2015)
  Chen HH, Welling M, Bloch DB, Muñoz J, Mientjes E, Chen X, Tramp C, Wu J, Yabuuchi A, Chou YF, Buecker C, Krainer A, Willemsen R, Heck AJ, Geijsen N
  Stem Cell Reports 3, 892-904.
• Fluctuations in histone H4 isoforms during cellular reprogramming monitored by middle-down proteomics.(2015)
  Benevento M, Tonge PD, Puri MC, Nagy A, Heck AJ, Munoz J
  Proteomics (in press).
• Genome-wide characterization of the routes to pluripotency.(2014)
  Hussein SM, Puri MC, Tonge PD, Benevento M, Corso AJ, Clancy JL, Mosbergen R, Li M, Lee DS, Cloonan N, Wood DL, Munoz J, Middleton R, Korn O, Patel HR, White CA, Shin JY, Gauthier ME, Lê Cao KA, Kim JI, Mar JC1 Shakiba N, Ritchie W, Rasko JE1, Grimmond SM, Zandstra PW, Wells CA, Preiss T, Seo JS, Heck AJ, Rogers IM, Nagy A
  Nature 516, 198-206.
• Inhibition of De Novo NAD+ Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage.(2014)
  Tummala KS, Gomes AL, Yilmaz , Graña O, Bakiri L, Ruppen I, Ximénez-Embún P, Sheshappanavar V, Rodriguez-Justo M, Pisano DG, Wagner EF, Djouder N
  Cancer Cell 26, 826-839.
• Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks.(2014)
  Benevento M, Tonge PD, Puri MC, Hussein SM, Cloonan N, Wood DL, Grimmond SM, Nagy A, Munoz J, Heck AJ
  Nat Communications 5, 5613-.
• Small RNA changes en route to distinct cellular states of induced pluripotency.(2014)
  Clancy JL, Patel HR, Hussein SM, Tonge PD, Cloonan N, Corso AJ, Li M, Lee DS, Shin JY, Wong JJ, Bailey CG, Benevento M, Munoz J, Chuah A, Wood D, Rasko JE, Heck AJ, Grimmond SM, Rogers IM, Seo JS, Wells CA, Puri MC, Nagy A, Preiss T
  Nat Communications 5, 5522-.
• From the Human Genome to the Human Proteome.(2014)
  Muñoz J, Heck AJ
  Angewandte Chemie 53, 10864-10866.
• A synthetic lethal interaction between APC/C and topoisomerase poisons uncovered by proteomic screens.(2014)
  Eguren M, Álvarez-Fernández M, García F, López-Contreras AJ, Fujimitsu K, Yaguchi H, Luque-García JL, Fernández-Capetillo O, Muñoz J, Yamano H, Malumbres M
  Cell Reports 6, 670-683.
• CUL4A contributes to the biology of basal-like breast tumors through modulation of cell growth and antitumor immune response.(2014)
  Saucedo-Cuevas LP, Ruppen I, Ximénez-Embún P, Domingo S, Gayarre J, Muñoz J, Silva JM, García MJ, Benítez J
  Oncotarget 5, 2330-2343.
• Next-generation proteomics: towards an integrative view of proteome dynamics.(2013)
  Altelaar AF, Munoz J, Heck AJ
  Nat Rev Genet 14, 35-48.
• S100A8/A9 (calprotectin) is a mediator in psoriasis controlling expression of complement factor C3.(2013)
  Schonthaler HB, Guinea-Viniegra J, Wculek SK, Ruppen I, Ximénez-Embún P, Guío-Carrión A, Navarro R, Hogg N, Ashman K, Wagner EF
  Immunity 39, 1171-1181.
• A proteomic characterization of factors enriched at nascent DNA molecules.(2013)
  López-Contreras AJ, Ruppen I, Nieto-Soler M, Murga M, Rodriguez- Acebes S, Remeseiro S, Rodrigo-Perez S, Rojas AM, Mendez J, Muñoz J, Fernandez-Capetillo O
  Cell Reports 3, 1-12.
• RAP1 protects from obesity through its extratelomeric role regulating gene expression.(2013)
  Martínez P, Gómez-López G, García F, Mercken E, Mitchell S, Flores JM, De Cabo R, Blasco MA
  Cell Reports 3, 2059-2074.
• Integrative analysis of miRNA and mRNA expression profiles in pheochromocytoma and paraganglioma identifies genotype-specific markers and potentially regulated pathways.(2013)
  de Cubas AA, Leandro-Garcia LJ, Schiavi F, Mancikova V, Comino-Méndez I, Inglada-Perez L, Pérez M, Ibarz N, Ximénez-Embún P, López-Jiménez E, Letón R, Maliszewska A, Gómez Graña A, Bernal C, Alvarez-Escolá C, Rodriguez-Antona C, Opocher G, Muñoz J, Megías D, Cascón A, Robledo M
  Endocr-Relat Cancer 20, 477-493.
• F-actin asymmetry and the endoplasmic reticulum-associated TCC-1 protein contribute to stereotypic spindle movements in the Caenorhabditis elegans embryo.(2013)
  Berends CW, Muñoz J, Portegijs V, Schmidt R, Grigoriev I, Boxem M, Akhmanova A, Heck AJ, van den Heuvel S
  Mol Biol Cell 24, 2201-2215.
• Finding the same needles in the haystack? A comparison of phosphotyrosine peptides enriched by immuno-affinity precipitation and metal-based affinity chromatography.(2013)
  Di Palma S, Zoumaro-Djayoon A, Peng M, Post H, Preisinger C, Munoz J, Heck AJ
  J Proteomics 91, 331-337.
• Shotgun proteomics of archival triple-negative breast cancer samples.(2013)
  Gámez-Pozo A, Ferrer NI, Ciruelos E, López-Vacas R, Martínez FG, Espinosa E, Vara JA
  Proteomics Clinical Applications 7, 283-291.