Unidad de Imagen Molecular

Francisca Mulero Jefe de Unidad
T +34 917328000 (Ext 3150)
fmulero@cnio.es

Técnicos de Laboratorio

Guillermo Garaulet
Guillermo Medrano

Molecular imaging techniques aim to characterise and quantify biological processes at the molecular and cellular levels, facilitating a repetitive, non-invasive, uniform, and relatively automated study of the same living subject using identical or alternative biological imaging assays at different time points. The statistical power of longitudinal studies is therefore harnessed, and the number of animals required and costs incurred are reduced. Combining techniques using multimodality (PET-CT, optical imaging-CT, and ultrasound) allows pathophysiological changes in early disease phases to be detected with high structural resolution. Other advantages include the ability to interrogate the whole body and to visualise the molecular target of interest in 3D space.

The services offered to CNIO researchers by the Molecular Imaging Unit cover different technologies to non-invasively and repetitively image targeted macromolecules in living organisms.

Publicaciones

González-Martínez J, Cwetsch AW, Gilabert-Juan J, Gómez J, Garaulet G, Schneider P, de Cárcer G, Mulero F, Caleiras E, Megías D, Porlan E, Malumbres M. (2022). Genetic interaction between PLK1 and downstream MCPH proteins in the control of centrosome asymmetry and cell fate during neural progenitor division. Cell Death Differ 29, 1474-1485. Publicación CNIO.
Torra J, Viela F, Megías D, Sot B, Flors C (2022). Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2. Chem-Eur J 28, e202200026. Publicación CNIO.
Gerke C, Zabala Gutierrez I, Méndez-González D, Cruz MCI, Mulero F, Jaque D, Rubio-Retama J (2022). Clickable Albumin Nanoparticles for Pretargeted Drug Delivery toward PD-L1 Overexpressing Tumors in Combination Immunotherapy. Bioconjugate Chem 33, 821-828. Publicación CNIO.
Mulero F, Oteo M, Garaulet G, Magro N, Rebollo L, Medrano G, Santiveri C, Romero E, Sellek RE, Margolles Y, Campos-Olivas R, Arroyo AG, Fernández LA, Morcillo MA, Martínez-Torrecuadrada JL (2022). Development of anti-membrane type 1-matrix metalloproteinase nanobodies as immunoPET probes for triple negative breast cancer imaging. Front Med 24, 1058455. Publicación CNIO. Open Access
Bertolin J, Sánchez V, Ribera A, Jaén ML, Garcia M, Pujol A, Sánchez X, Muñoz S, Marcó S, Pérez J, Elias G, León X, Roca C, Jimenez V, Otaegui P, Mulero F, Navarro M, Ruberte J, Bosch F. (2021). Treatment of skeletal and non-skeletal alterations of Mucopolysaccharidosis type IVA by AAV-mediated gene therapy. Nat Commun 12, 5343. Publicación CNIO. Open Access
de la Calle Arregui C, Plata-Gómez AB, Deleyto-Seldas N, García F, Ortega-Molina A, Abril-Garrido J, Rodriguez E, Nemazanyy I, Tribouillard L, de Martino A, Caleiras E, Campos-Olivas R, Mulero F, Laplante M, Muñoz J, Pende M, Sabio G, Sabatini DM, Efeyan A (2021). Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. Nat Commun 12, 3660. Publicación CNIO. Open Access
Garlito-Díaz H, Esteban J, Mediero A, Carias-Cálix RA, Toirac B, Mulero F, Faus-Rodrigo V, Jiménez-Morales A, Calvo E, Aguilera-Correa JJ. (2021). A New Antifungal-Loaded Sol-Gel Can Prevent Candida albicans Prosthetic Joint Infection. Antibiotics-Basel 10, 711. Publicación CNIO.
Mora-Raimundo P, Lozano D, Benito M, Mulero F, Manzano M, Vallet-Regí M. (2021). Osteoporosis Remission and New Bone Formation with Mesoporous Silica Nanoparticles. Adv Sci 8, e2101107. Publicación CNIO.
Shaikh F, Andersen MB, Sohail MR, Mulero F, Awan O, Dupont-Roettger D, Kubassova O, Dehmeshki J, Bisdas S (2021). Current Landscape of Imaging and the Potential Role for Artificial Intelligence in the Management of COVID-19. Curr Probl Diagn Radiol 50, 430-435. Publicación CNIO.
Quintela-Fandino M, Morales S, Cortés-Salgado A, Manso L, Apala JV, Muñoz M, Gasol Cudos A, Salla Fortuny J, Gion M, Lopez-Alonso A, Cortés J, Guerra J, Malón D, Caleiras E, Mulero F, Mouron S (2020). Randomized Phase 0/I Trial of the Mitochondrial Inhibitor ME-344 or Placebo Added to Bevacizumab in Early HER2-Negative Breast Cancer.. Clin Cancer Res 26, 35-45. Publicación CNIO.
Aguilera-Correa JJ, Garcia-Casas A, Mediero A, Romera D, Mulero F, Cuevas-López I, Jiménez-Morales A, Esteban J (2020). A new antibiotic-loaded sol-gel can prevent bacterial prosthetic joint infection: from in vitro studies to an in vivo model.. Front Microbiol 17, 2935. Publicación CNIO.
Conesa-Buendía FM, Mediero A, Fujikawa R, Esbrit P, Mulero F, Mahillo-Fernández I, Mues AO (2020). Beneficial effects of manually assisted chiropractic adjusting instrument in a rabbit model of osteoarthritis. Sci Rep 10, 1237. Publicación CNIO. Open Access
Shaikh F, Andersen MB, Sohail MR, Mulero F, Awan O, Dupont-Roettger D, Kubassova O, Dehmeshki J, Bisdas S (2020). Current landscape of imaging and the potential role for artificial intelligence in the management of COVID-19.. Curr Probl Diagn Radiol (in press). Publicación CNIO.
Blasco MT, Navas C, Martín-Serrano G, Graña-Castro O, Lechuga CG, Martín-Díaz L, Djurec M, Li Jing, Morales-Cacho L, Esteban-Burgos L, Perales-Patón J, Bousquet-Mur E, Castellano E, Jacob Harrys KC, Cabras L, Mustenau M, Drosten M, Ortega S, Mulero F, Sainz B, Dusetti N, Iovanna J, Sánchez-Bueno F, Hidalgo M, Khiabanian H, Hidalgo M, Rabadán R, Al-Shahrour F, Guerra C, Barbacid M (2019). Complete Regression of Advanced Pancreatic Ductal Adenocarcinomas upon Combined Inhibition of EGFR and C-RAF. Cancer Cell 35, 573-587. Publicación CNIO.
Garaulet G, Pérez-Chacon G, Alarcón H, Alfranca A, Mulero F, Martínez-Torrecuadrada J, Zapata JM, Rodríguez A (2019). Intratumoral expression using a NFkB-based promoter enhances IL12 antitumor efficacy. Cancer Gene Ther 26, 216-233. Publicación CNIO.
Cussó L, Musteanu M, Mulero F, Barbacid M, Desco M (2019). Effects of a Ketogenic Diet on [18F]FDG-PET Imaging in a Mouse Model of Lung Cancer. Mol Imaging Biol 21, 279-285. Publicación CNIO.
Jimenez V, Jambrina C, Casana E, Sacristan V, Muñoz S, Darriba S, Rodó J, Mallol C, Garcia M, León X, Marcó S, Ribera A, Elias I, Casellas A, Grass I, Elias G, Ferré T, Motas S, Franckhauser S, Mulero F, Navarro M, Haurigot V, Ruberte J, Bosch F (2018). FGF21 gene therapy as treatment for obesity and insulin resistance. EMBO Mol Med 10, e8791. Publicación CNIO. Open Access
Muñoz-Espín D, Rovira M, Galiana I, Giménez C, Lozano-Torres B, Paez-Ribes M, Llanos S, Chaib S, Muñoz-Martín M, Ucero AC, Garaulet G, Mulero F, Dann SG, VanArsdale T, Shields DJ, Bernardos A, Murguía JR, Martínez-Máñez R, Serrano M (2018). A versatile drug delivery system targeting senescent cells. EMBO Mol Med 10, pii: e9355. Publicación CNIO. Open Access
Gambera S, Abarrategi A, Rodríguez-Milla MA, Mulero F, Menéndez ST, Rodriguez R, Navarro S, García-Castro J (2018). Role of Activator Protein-1 Complex on the Phenotype of Human Osteosarcomas Generated from Mesenchymal Stem Cells. Stem Cells 36, 1487-1500. Publicación CNIO.
Cussó L, Musteanu M, Mulero F, Barbacid M, Desco M (2018). Effects of a Ketogenic Diet on [18F]FDG-PET Imaging in a Mouse Model of Lung Cancer. Mol Imaging Biol 21, 279-285. Publicación CNIO.
Morcillo MÁ, García de Lucas Á, Oteo M, Romero E, Magro N, Ibáñez M, Martínez A, Garaulet G, Arroyo AG, López-Casas PP, Hidalgo M, Mulero F, Martínez-Torrecuadrada J (2018). MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management. Contrast Media & Molecular Imaging 2018, 8382148. Publicación CNIO. Open Access