Unidad de Anticuerpos Monoclonales

Giovanna Roncador Jefe de Unidad
T +34 917328000 (Ext 3130)
groncador@cnio.es

Técnicos de Laboratorio

Scherezade Jiménez
Lorena Maestre
Ana Isabel Reyes
Carmen San Juan

Monoclonal antibodies (mAbs) have become indispensable in biomedicine, revolutionising our approach to understanding and addressing complex biological processes. By providing researchers with a reliable and precise tool, mAbs contribute significantly to the advancement of our knowledge about the mechanisms involved in tumour transformation and development. Beyond their fundamental role in laboratory research, mAbs play a crucial part in cancer detection and therapy, offering practical solutions for its diagnosis and treatment.

The Monoclonal Antibodies Unit provides CNIO Research Groups with the “à la carte” generation of mAbs using hybridoma technology. We are highly specialised in the production of mouse and rat monoclonal antibodies. The Unit also offers mAb characterisation and validation, medium scale mAb production, as well as a service of Mycoplasma testing for the cell culture facility.

Publicaciones

Carreras J, Ikoma H, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Kondo Y, Ito A, Nagase S, Miura H, Kawada H, Roncador G, Campo E, Hamoudi R, Nakamura N. (2024). Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement. Virchows Arch 484, 657-676. Publicación CNIO.
Larrayoz M, Garcia-Barchino MJ, Celay J, Etxebeste A, Jimenez M, Perez C, Ordoñez R, Cobaleda C, Botta C, Fresquet V, Roa S, Goicoechea I, Maia C, Lasaga M, Chesi M, Bergsagel PL, Larrayoz MJ, Calasanz MJ, Campos-Sanchez E, Martinez-Cano J, Panizo C, Rodriguez-Otero P, Vicent S, Roncador G, Gonzalez P, Takahashi S, Katz SG, Walensky LD, Ruppert SM, Lasater EA, Amann M, Lozano T, Llopiz D, Sarobe P, Lasarte JJ, Planell N, Gomez-Cabrero D, Kudryashova O, Kurilovich A, Revuelta MV, Cerchietti L, Agirre X, San Miguel J, Paiva B, Prosper F, Martinez-Climent JA. (2023). Preclinical models for prediction of immunotherapy outcomes and immune evasion mechanisms in genetically heterogeneous multiple myeloma. Nat Med 29, 632-645. Publicación CNIO.
Del Rio ML, de Juan CY, Roncador G, Caleiras E, Álvarez-Esteban R, Pérez-Simón JA, Rodriguez-Barbosa JI. (2023). Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1- stem cell-like T cells over PD-1+ T cells curbing tumor progression. Front Inmmunol 14, 1113858. Publicación CNIO.
Fernández S, Solórzano JL, Díaz E, Menéndez V, Maestre L, Palacios S, López M, Colmenero A, Estévez M, Montalbán C, Martínez Á, Roncador G, García JF (2023). JAK/STAT blockade reverses the malignant phenotype of Hodgkin and Reed-Sternberg cells. Blood Adv 7, 4135-4147. Publicación CNIO. Open Access
Roldán-Romero JM, Valdivia C, Santos M, Lanillos J, Maroto P, Anguera G, Calsina B, Martinez-Montes A, Monteagudo M, Mellid S, Leandro-García LJ, Montero-Conde C, Cascón A, Roncador G, Coloma J, Robledo M, Rodriguez-Antona C. (2023). Deubiquitinase USP9X loss sensitizes renal cancer cells to mTOR inhibition. Int J Cancer 153, 1300-1312. Publicación CNIO.
Carreras J, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Nagase S, Miura H, Roncador G, Colomo L, Hamoudi R, Campo E, Nakamura N. (2023). Mutational Profile and Pathological Features of a Case of Interleukin-10 and RGS1-Positive Spindle Cell Variant Diffuse Large B-Cell Lymphom. Hematol Rep 15, 188-220. Publicación CNIO.
Yamamoto Y, Carreras J, Shimizu T, Kakizaki M, Kikuti YY, Roncador G, Nakamura N, Kotani A (2022). Anti-HBV drug entecavir ameliorates DSS-induced colitis through PD-L1 induction. Pharmacol Res 179, 105918. Publicación CNIO.
Carreras J, Kikuti YY, Hiraiwa S, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Itoh J, Roncador G, Martinez A, Colomo L, Hamoudi R, Ando K, Nakamura N (2022). High PTX3 expression is associated with a poor prognosis in diffuse large B-cell lymphoma. Cancer Sci 113, 334-348. Publicación CNIO. Open Access
Carreras J, Roncador G, Hamoudi R. (2022). Artificial Intelligence Predicted Overall Survival and Classified Mature B-Cell Neoplasms Based on Immuno-Oncology and Immune Checkpoint Panels. Cancers 14, 5318. Publicación CNIO. Open Access
Fernández S, Solórzano JL, Díaz E, Menéndez V, Maestre L, Palacios S, López M, Velázquez AC, Estévez M, Montalban C, Martinez-Ramirez A, Roncador G, García JF. (2022). JAK/STAT blockade reverses the malignant phenotype of Hodgkin and Reed-Sternberg cells. Blood Adv (in press). Publicación CNIO. Open Access
Miyaoka M, Kikuti YY, Carreras J, Ito A, Ikoma H, Tomita S, Kawada H, Roncador G, Bea S, Campo E, Nakamura N. (2022). Copy Number Alteration and Mutational Profile of High-Grade B-Cell Lymphoma with MYC and BCL2 and/or BCL6 Rearrangements, Diffuse Large B-Cell Lymphoma with MYC-Rearrangement, and Diffuse Large B-Cell Lymphoma with MYC-Cluster Amplification. Cancers 14, 5849. Publicación CNIO. Open Access
Roncador G, Puñet-Ortiz J, Maestre L, Rodríguez-Lobato LG, Jiménez S, Reyes-García AI, García-González Á, García JF, Piris MÁ, Montes-Moreno S, Rodríguez-Justo M, Mena MP, Fernández de Larrea C, Engel P. (2022). CD229 (Ly9) a Novel Biomarker for B-Cell Malignancies and Multiple Myeloma.. Cancers 14, 2154. Publicación CNIO. Open Access
Righi S, Novero D, Godio L, Bertuzzi C, Bacci F, Agostinelli C, Sagramoso C, Rossi M, Piccioli M, Gazzola A, Mannu C, Roncador G, Sabattini E. (2022). Myeloid nuclear differentiation antigen: an aid in differentiating lymphoplasmacytic lymphoma and splenic marginal zone lymphoma in bone marrow biopsies at presentation. Hum Pathol 124, 67-75. Publicación CNIO.
Peña-Cardelles JF, Pozo-Kreilinger JJ, Roncador G, Esteban-Hernández J, Moro-Rodríguez JE, Sastre-Perona A, Castelo-Fernández B, Cebrián-Carretero JL. (2022). Prognosis Value of Immunoregulatory Molecules in Oral Cancer Microenvironment: An Immunohistochemical Study. Biomedicines 10, 710. Publicación CNIO.
Pileri A, Cavicchi M, Bertuzzi C, Righi S, Zengarini C, Sabattini E, Roncador G, Agostinelli C. (2022). TOX Expression in Mycosis Fungoides and Sezary Syndrome.. Diagnostics 12, 1582. Publicación CNIO. Open Access
Carreras J, Hiraiwa S, Kikuti YY, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Roncador G, Garcia JF, Ando K, Hamoudi R, Nakamura N (2021). Artificial neural networks predicted the overall survival and molecular subtypes of diffuse large B-cell lymphoma using a pancancer immune-oncology panel.. Cancers 13, 6384. Publicación CNIO.
Carreras J, Kikuti YY, Hiraiwa S, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Itoh J, Roncador G, Martinez A, Colomo L, Hamoudi R, Ando K, Nakamura N. (2021). High PTX3 expression is associated with a poor prognosis in diffuse large B-cell lymphoma. Cancer Sci 113, 334-348. Publicación CNIO.
Monteagudo M, Martínez P, Leandro-García LJ, Martínez-Montes ÁM, Calsina B, Pulgarín-Alfaro M, Díaz-Talavera A, Mellid S, Letón R, Gil E, Pérez-Martínez M, Megías D, Torres-Ruiz R, Rodriguez-Perales S, González P, Caleiras E, Jiménez-Villa S, Roncador G, Álvarez-Escolá C, Regojo RM, Calatayud M, Guadalix S, Currás-Freixes M, Rapizzi E, Canu L, Nölting S, Remde H, Fassnacht M, Bechmann N, Eisenhofer G, Mannelli M, Beuschlein F, Quinkler M, Rodríguez-Antona C, Cascón A, Blasco MA, Montero-Conde C, Robledo M (2021). Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma. Cancers 13, 4758. Publicación CNIO. Open Access
Voskuil JLA, Bandrowski A, Begley CG, Bradbury ARM, Chalmers AD, Gomes AV, Hardcastle T, Lund-Johansen F, Plückthun A, Roncador G, Solache A, Taussig MJ, Trimmer JS, Williams C, Goodman (2020). The Antibody Society’s antibody validation webinar series.. MABS 12, 1794421. Publicación CNIO.
Maestre L, García-García JF, Jiménez S, Reyes-García AI, García-González Á, Montes-Moreno S, Arribas AJ, González-García P, Caleiras E, Banham AH, Piris MÁ, Roncador G. (2020). High-mobility group box (TOX) antibody a useful tool for the identification of B and T cell subpopulations. PLoS One 15, e0229743. Publicación CNIO. Open Access
Tsuda S, Carreras J, Kikuti Y, Nakae H, Dekiden-Monma M, Imai J, Tsuruya K, Nakamura J, Tsukune Y, Uchida T, Matsushima M, Roncador G, Suzuki T, Nakamura N, Mine T (2019). Prediction of steroid demand in the treatment of patients with ulcerative colitis by immunohistochemical analysis of the mucosal microenvironment and immune checkpoint: role of macrophages and regulatory markers in disease severity. Pathol Int 69, 260-271. Publicación CNIO.
Carreras J, Lopez-Guillermo A, Kikuti YY, Itoh J, Masashi M, Ikoma H, Tomita S, Hiraiwa S, Hamoudi R, Rosenwald A, Leich E, Martinez A, Roncador G, Villamor N, Colomo L, Perez P, Tsuji NM, Campo E, Nakamura N (2019). High TNFRSF14 and low BTLA are associated with poor prognosis in Follicular Lymphoma and in Diffuse Large B-cell Lymphoma transformation. J Clin Exp Hematop 59, 1-16. Publicación CNIO.