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Investigadores
- Jorge Luís Martínez
Becarios Post-doctorales
- César Rodríguez
Técnicos de Laboratorio
- Aida Contreras
- Diana Martín
- Pilar Redondo
The Crystallography and Protein Engineering Unit (XTPEUnit) is a core facility that provides on-demand services at different levels, from protein cloning to the determination of its 3D structure, with the purpose to fulfil the demands of our users and to comprehend how their protein targets work. Thus, we produce proteins for different types of biochemical/biophysical/in vitro/in vivo assays, for monoclonal antibody generation, and we also offer macromolecular structural determination at low resolution by small-angle X-ray scattering (SAXS) or at atomic resolution by X-ray crystallography. The latter includes protein co-crystallisation in the presence of inhibitors or small fragments − a method that we routinely combine in parallel with the quantification of protein thermal stability (thermofluor assay) − during the guided drug discovery process.
Publicaciones
- (2020). Pyrido[2,3-b][1,5]benzoxazepin-5(6H)-one derivatives as CDK8 inhibitors.. Eur J Med Chem 201, 112443. Publicación CNIO.
- (2020). EphrinB2-EphB4 signalling provides Rho-mediated homeostatic control of lymphatic endothelial cell junction integrity. Elife 8, e57732. Publicación CNIO.
- (2019). Structural and biochemical insights into an engineered high-redox potential laccase overproduced in Aspergillus.. Int J Biol Macromol 141, 855-866. Publicación CNIO.
- (2019). Intratumoral expression using a NFkB-based promoter enhances IL12 antitumor efficacy. Cancer Gene Ther 26, 216-233. Publicación CNIO.
- (2019). Pancreatic Tumor Microenvironment Modulation by EphB4-ephrinB2 Inhibition and Radiation Combination. Clin Cancer Res 25, 3352-3365. Publicación CNIO.
- (2018). Exome Sequencing of Plasma DNA Portrays the Mutation Landscape of Colorectal Cancer and Discovers Mutated VEGFR2 Receptors as Modulators of Antiangiogenic Therapies. Clin Cancer Res 24, 3550-3559. Publicación CNIO.
- (2018). MT1-MMP as a PET Imaging Biomarker for Pancreas Cancer Management. Contrast Media & Molecular Imaging 2018, 8382148. Publicación CNIO.
Open Access
- (2018). A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun 9, 4809. Publicación CNIO.
Open Access
- (2018). Molecular basis of Tousled-Like Kinase 2 activation. Nat Commun 9, 25-35. Publicación CNIO.
Open Access
- (2017). Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine.. Nature 546, 676-680. Publicación CNIO.
- (2017). Modulation of telomere protection by the PI3K/AKT pathway. Nat Commun 8, 1278. Publicación CNIO.
Open Access
- (2017). Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme.. PLoS ONE 12, e0174454. Publicación CNIO.
- (2017). A mechanism for cancer-associated inactivation of NQO1 due to P187S and its reactivation by the consensus mutation H80R.. FEBS Lett 591, 2826-2835. Publicación CNIO.
- (2017). Receptor-based virtual screening and biological characterization of human apurinic/apyrimidinic endonuclease (Ape1) inhibitors.. ChemMedChem 7, 2168-2178. Publicación en otras instituciones.
- (2017). Display of the albumin binding domain in the envelope improves lentiviral vector bioavailability.. Hum Gene Ther Methods 28, 340-351. Publicación CNIO.
- (2016). Targeting MT1-MMP as an ImmunoPET-Based Strategy for Imaging Gliomas. PLoS One 11, e0158634. Publicación CNIO.
Open Access
- (2016). EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells.. Elife pii, e14845. Publicación CNIO.
- (2016). Changes in the folding landscape of the WW domain provide a molecular mechanism for an inherited genetic syndrome. Sci Rep 6, 30293. Publicación CNIO.
Open Access
- (2016). Molecular Architecture of Full Length TRF1 Favors its Interaction with DNA.. J Biol Chem 291, 21829-21835. Publicación CNIO.
- (2016). An Allosteric Cross-Talk Between the Activation Loop and the ATP Binding Site Regulates the Activation of Src Kinase. Sci Rep 6, 24235. Publicación CNIO.
Open Access
- (2016). Experimental and computational evidence on conformational fluctuations as a source of catalytic defects in genetic diseases.. RSC ADVANCES 6, 58604-58612. Publicación CNIO.
- (2016). Structural and dynamics studies of human phenylalanine hydroxilase, a highly regulated allosteric enzime.. Acta Crystallogr A 72, S239. Publicación CNIO.
- (2016). Galectin-related protein: An integral member of the network of chicken galectins 1. From strong sequence conservation of the gene confined to vertebrates to biochemical characteristics of the chicken protein and its crystal structure.. Biochim Biophys Acta 1860, 2285-2297. Publicación en otras instituciones.
- (2016). The structure of VgrG1 from Pseudomonas aeruginosa, the needle tip of the bacterial type VI secretion system.. Acta Crystallogr D Struct Biol 72, 22-33. Publicación en otras instituciones.