Biotechnology Programme

Transgenic Mice Core Unit

Head of Unit:  Sagrario Ortega

Genetically engineered mice are an essential tool for analysing the molecular mechanisms underlying tumour development and cancer biology. Modelling cancer by modifying the germ line of the mouse has become a crucial component of drug discovery as well as for the assessment of experimental therapies at the preclinical stage. The Transgenic Mice Unit at the CNIO offers state-of-theart technology for the manipulation of the mouse genome. Using classical transgenesis, homologous recombination in embryonic stem cells and genome editing by targeted nucleases, the Unit has generated more than 300 mutant alleles of cancer related genes in the mouse germ line. The Unit also provides support and collaborates with CNIO researchers in many aspects related to research with embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and embryo- and mouse model-based research. Finally, the Unit also leads its own research projects focused on the generation of mouse models to study tumour biology, as well as on the screening of cancer-related genes.