The pattern of DNA methylation is crucial for maintaining cellular function, and abnormalities in DNA methylation, whether in the form of hypermethylation or hypomethylation, can lead to adverse outcomes such as cancer or other diseases.

There are available different solutions, depending on the specific objectives of the research project, the scale of analysis required and the available resources:

Methylation analyisis by genotyping

Infinium Human Methylation EPIC Array

Epigenetic analysis is performed with the Infinium MethylationEPIC v2.0 BeadChip, which is a genome-wide methylation screening tool that targets over 935,000 CpG sites in the most biologically significant regions of the human methylome. The array maintains the ability to quantitatively interrogate CpGs across the genome at single-nucleotide resolution, while providing highly accurate and precise methylation measurements independently of read depth.

Multiple DNA sample types, including those isolated from FFPE, can be analyzed using the streamlined, user-friendly Infinium Methylation assay.

For more information visit: https://www.illumina.com/products/by-type/microarray-kits/infinium-methylation-epic.html

Infinium Methylation Screening Array

The Infinium Methylation Screening Array features 270K methylation sites focused on CpG regions associated with a range of common cell and organism traits, such as cell identity, nonmalignant disease phenotypes, and environmental exposures. Beadchip content was selected for having powerful published trait associations from a combination of Infinium methylation studies, functional genomics sequencing studies, and updated genomic databases. the focused design and higher sample throughput of the Infinium Methylation Screening array enables researchers to apply DNa methylation screening to large population health projects to discover disease targets.

For more information visit: https://www.illumina.com/products/by-type/microarray-kits/infinium-methylation-screening-array.html

Infinium Mouse Methylation Array

The Infinium Mouse Methylation BeadChip offers a unique combination of comprehensive, expert-selected coverage and high sample throughput. It is ideal for genome-wide DNA methylation studies with a large number of samples. The BeadChip interrogates > 285k methylation sites per sample at single-nucleotide resolution. It provides balanced coverage of CpG islands, translation start sites, enhancers, imprinted loci, and other regions.

For more information visit: https://emea.illumina.com/products/by-type/microarray-kits/infinium-mouse-methylation.html

Methylation by sequencing

Coming soon, H2

Other epigenomic applications

Other epigenomic applications based on NGS please contact with us: cegen@cnio.es