Home | Research & Innovation | Scientific Programmes | Structural Biology Programme | Protein Production Unit
The functions of many proteins, particularly those associated with cancer, remain poorly understood. Progress in biomedical research depends on determining protein structures, defining their distribution within cells and tissues, and elucidating their biological roles. A major bottleneck, however, is the difficulty of producing proteins of interest in sufficient quality and quantity for comprehensive analysis, which hampers research progress.
The Protein Production Unit was established to address this limitation. This core facility provides specialised expertise and advanced technologies to develop efficient pipelines for the production of high-quality recombinant proteins. These proteins are essential resources for a variety of downstream applications, including the generation of highly specific antibodies, biophysical and biochemical characterisation, functional assays, and structural studies. Ultimately, the Unit aims to accelerate cancer research at CNIO and support external research groups.
Recent publications
- Garaulet G, Báez BB, Medrano G, Rivas-Sánchez M, Sánchez-Alonso D, Martinez-Torrecuadrada JL, Mulero F. (2024). Radioimmunotheragnosis in Cancer Research. Cancers 16, 2896. CNIO Publication.
- Magro N, Oteo M, Romero E, Ibáñez-Moragues M, Lujan VM, Martínez L, Vela O, López-Melero ME, Arroyo AG, Garaulet G, Martínez-Torrecuadrada JL, Mulero F, Morcillo MA. (2024). Target engagement of an anti-MT1-MMP antibody for triple-negative breast cancer PET imaging and beta therapy. Nucl Med Biol 136-137, 108930. CNIO Publication.
- Laguía O, Bosso G, Martínez-Torrecuadrada J, Míguez-Amil S, Fernández-Leiro R, Blasco MA. (2024). Protocol for the generation and purification of high-molecular-weight covalent RNA-DNA hybrids with T4 RNA ligase. Star Protoc 5, 102930. CNIO Publication.
- García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. (2022). Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. Mol Cell 82, 3438-3452. CNIO Publication.
Open Access - Mulero F, Oteo M, Garaulet G, Magro N, Rebollo L, Medrano G, Santiveri C, Romero E, Sellek RE, Margolles Y, Campos-Olivas R, Arroyo AG, Fernández LA, Morcillo MA, Martínez-Torrecuadrada JL (2022). Development of anti-membrane type 1-matrix metalloproteinase nanobodies as immunoPET probes for triple negative breast cancer imaging. Front Med 24, 1058455. CNIO Publication. Open Access
- Sanclemente M, Nieto P, Garcia-Alonso S, Fernández-García F, Esteban-Burgos L, Guerra C, Drosten M, Caleiras E, Martinez-Torrecuadrada J, Santamaría D, Musteanu M, Barbacid M (2021). RAF1 kinase activity is dispensable for KRAS/p53 mutant lung tumor progression.. Cancer Cell 39, 294-296. CNIO Publication. Open Access
- Compte M, Harwood SL, Martínez-Torrecuadrada J, Perez-Chacon G, González-García P, Tapia-Galisteo A, Van Bergen En Henegouwen PMP, Sánchez A, Fabregat I, Sanz L, Zapata JM, Alvarez-Vallina L (2021). Case report: an EGFR-targeted 4-1BB-agonistic trimerbody does not induce hepatotoxicity in transgenic mice with liver expression of human EGFR.. Front Inmmunol 11, 614363. CNIO Publication.
