Home | Research & Innovation | Scientific Programmes | Structural Biology Programme | Protein Production Unit
The roles of numerous proteins, particularly those linked to cancer, remain largely unexplored. Advancing biomedical research requires mapping these proteins´ structures, understanding where and how they are distributed within tissues and cells, and uncovering their specific functions. However, a major obstacle is the difficulty of producing proteins of interest in sufficient quality and quantity for thorough analysis, which slows down research.
The Protein Production Unit was created to tackle this challenge. This core lab provides expertise and advanced technology to develop streamlined methods for producing high quality recombinant proteins. These proteins serve as essential resources for a range of downstream applications such as the production of highly specific antibodies, detailed biophysical and biochemical analyses, functional studies, and structural investigations. Ultimately, this Unit aims to accelerate cutting-edge cancer research at CNIO and support external groups.
Recent publications
- Garaulet G, Báez BB, Medrano G, Rivas-Sánchez M, Sánchez-Alonso D, Martinez-Torrecuadrada JL, Mulero F. (2024). Radioimmunotheragnosis in Cancer Research. Cancers 16, 2896. CNIO Publication.
- Magro N, Oteo M, Romero E, Ibáñez-Moragues M, Lujan VM, Martínez L, Vela O, López-Melero ME, Arroyo AG, Garaulet G, Martínez-Torrecuadrada JL, Mulero F, Morcillo MA. (2024). Target engagement of an anti-MT1-MMP antibody for triple-negative breast cancer PET imaging and beta therapy. Nucl Med Biol 136-137, 108930. CNIO Publication.
- Laguía O, Bosso G, Martínez-Torrecuadrada J, Míguez-Amil S, Fernández-Leiro R, Blasco MA. (2024). Protocol for the generation and purification of high-molecular-weight covalent RNA-DNA hybrids with T4 RNA ligase. Star Protoc 5, 102930. CNIO Publication.
- García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. (2022). Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. Mol Cell 82, 3438-3452. CNIO Publication.
Open Access - Mulero F, Oteo M, Garaulet G, Magro N, Rebollo L, Medrano G, Santiveri C, Romero E, Sellek RE, Margolles Y, Campos-Olivas R, Arroyo AG, Fernández LA, Morcillo MA, Martínez-Torrecuadrada JL (2022). Development of anti-membrane type 1-matrix metalloproteinase nanobodies as immunoPET probes for triple negative breast cancer imaging. Front Med 24, 1058455. CNIO Publication. Open Access
- Sanclemente M, Nieto P, Garcia-Alonso S, Fernández-García F, Esteban-Burgos L, Guerra C, Drosten M, Caleiras E, Martinez-Torrecuadrada J, Santamaría D, Musteanu M, Barbacid M (2021). RAF1 kinase activity is dispensable for KRAS/p53 mutant lung tumor progression.. Cancer Cell 39, 294-296. CNIO Publication. Open Access
- Compte M, Harwood SL, Martínez-Torrecuadrada J, Perez-Chacon G, González-García P, Tapia-Galisteo A, Van Bergen En Henegouwen PMP, Sánchez A, Fabregat I, Sanz L, Zapata JM, Alvarez-Vallina L (2021). Case report: an EGFR-targeted 4-1BB-agonistic trimerbody does not induce hepatotoxicity in transgenic mice with liver expression of human EGFR.. Front Inmmunol 11, 614363. CNIO Publication.
