Monoclonal Antibodies Core Unit

Giovanna Roncador Head of Unit
T +34 917328000 (Ext 3130)
groncador@cnio.es

Technicians

Lorena Maestre
Ana Isabel Reyes
Andrea Belén Romero

The application of hybridoma technology for the production of monoclonal antibodies ( mAbs ) represents one of the most relevant methodological advances in biomedicine of the past decades. The availability of monoclonal antibodies has significantly improved our knowledge about the processes involved in tumour generation and development, opening up a vast array of new possibilities for basic and applied research.

The Monoclonal Antibodies Unit provides CNIO Research Groups with an ‘a la carte’ generation of mAbs. We are highly specialised in the production of mouse and rat monoclonal antibodies. The Unit also offers mAb characterisation and validation, medium-scale mAb production, as well as a service of Mycoplasma testing for the cell culture facility.

Publications

Tsuda S, Carreras J, Kikuti Y, Nakae H, Dekiden-Monma M, Imai J, Tsuruya K, Nakamura J, Tsukune Y, Uchida T, Matsushima M, Roncador G, Suzuki T, Nakamura N, Mine T (2019). Prediction of steroid demand in the treatment of patients with ulcerative colitis by immunohistochemical analysis of the mucosal microenvironment and immune checkpoint: role of macrophages and regulatory markers in disease severity. Pathol Int (in press). CNIO Publication.
Carreras J, Yukie Kikuti Y, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Shiraiwa S, Ando K, Sato S, Suzuki Y, Miura I, Roncador G, Nakamura N (2018). Genomic Profile and Pathologic Features of Diffuse Large B-Cell Lymphoma Subtype of Methotrexate-associated Lymphoproliferative Disorder in Rheumatoid Arthritis Patients. Am J Surg Pathol 42, 936-950. CNIO Publication.
Manso R, González-Rincón J, Rodríguez-Justo M, Roncador G, Gómez S, Sánchez-Beato M, Piris MA, Rodríguez-Pinilla SM (2018). Overlap at the molecular and immunohistochemical levels between angioimmunoblastic T-cell lymphoma and a subgroup of peripheral T-cell lymphomas without specific morphological features. Oncotarget 9, 16124-16133. CNIO Publication. Open Access
Vojkovics D, Kellermayer Z, Kajtár B, Roncador G, Vincze Á, Balogh P (2018). Nkx2-3-A Slippery Slope From Development Through Inflammation Toward Hematopoietic Malignancies. Biomark Insights 13, 1-6. CNIO Publication. Open Access
Manso R, Roncador G, Montes-Moreno S, Rojo F, Pérez-Sáenz MÁ, Mollejo M, Menárguez J, Carvajal N, García-Cosio M, Llamas P, Piris MA, Rodríguez-Pinilla SM (2017). p-MAPK1 expression associated with poor prognosis in angioimmunoblastic T-cell lymphoma patients.. Br J Haematol 176, 661-664. CNIO Publication.
Carreras J, Kikuti YY, Beà S, Miyaoka M, Hiraiwa S, Ikoma H, Nagao R, Martin-Garcia D, Salaverria I, Sato A, Akifumi I, Roncador G, Garcia JF, Ando K, Campo E, Nakamura N (2017). Clinicopathological characteristics and genomic profile of primary sinonasal tract diffuse large B-cell lymphoma (DLBCL) reveals gain at 1q31 and RGS1 encoding protein; high RGS1 immunohistochemical expression associates with poor overall survival in DLBCL NOS.. Histopathology 70, 595-621. CNIO Publication.
Kunder CA, Roncador G, Advani RH, Gualco G, Bacchi CE, Sabile JM, Lossos IS, Nie K, Tibshirani RJ, Green MR, Alizadeh AA, Natkunam Y (2017). KLHL6 is preferentially expressed in germinal center-derived B-cell lymphomas.. Am J Clin Pathol 148, 465-476. CNIO Publication.
Tavera-Tapia A, Pérez-Cabornero L, Macías JA, Ceballos MI, Roncador G, de la Hoya M, Barroso A, Felipe-Ponce V, Serrano-Blanch R, Hinojo C, Miramar-Gallart MD, Urioste M, Caldés T, Santillan-Garzón S, Benitez J, Osorio A (2017). Almost 2% of Spanish breast cancer families are associated to germline pathogenic mutations in the ATM gene.. Breast Cancer Res Treat 161, 117-134. CNIO Publication.
Wong KK, Gascoyne DM, Soilleux EJ, Lyne L, Spearman H, Roncador G, Pedersen LM, Møller MB, Green TM, Banham AH (2016). FOXP2-positive diffuse large B-cell lymphomas exhibit a poor response to R-CHOP therapy and distinct biological signatures.. Oncotarget 7, 52940-52956. CNIO Publication.
Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS, Šerbec VC, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa JI, Torensma R, Turley H, Pulford K, Banham AH (2016). The European antibody network’s practical guide to finding and validating suitable antibodies for research.. MABS 8, 27-36. CNIO Publication.
Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS (2016). Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Nat Commun 7, 13418. CNIO Publication. Open Access
Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, Reyes-García AI, Rubio C, Tomás JF, Estévez M, Pulford K, Piris MA, García JF. (2015). CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.. PLoS ONE 10, e0125203. CNIO Publication.
Cascón A, Comino-Méndez I, Currás-Freixes M, de Cubas AA, Contreras L, Richter S, Peitzsch M, Mancikova V, Inglada-Pérez L, Pérez-Barrios A, Calatayud M, Azriel S, Villar-Vicente R, Aller J, Setién F, Moran S, Garcia JF, Río-Machín A, Letón R, Gómez-Graña Á, Apellániz-Ruiz M, Roncador G, Esteller M, Rodríguez-Antona C, Satrústegui J, Eisenhofer G, Urioste M, Robledo M (2015). Whole-Exome Sequencing Identifies MDH2 as a New Familial Paraganglioma Gene.. J Natl Cancer I 107, djv053. CNIO Publication.
Maraver A, Fernandez-Marcos PJ, Cash TP, Mendez-Pertuz M, Dueñas M, Maietta P, Martinelli P, Muñoz-Martin M, Martínez-Fernández M, Cañamero M, Roncador G, Martinez-Torrecuadrada JL, Grivas D, de la Pompa JL, Valencia A, Paramio JM, Real FX, Serrano M (2015). NOTCH pathway inactivation promotes bladder cancer progression.. J Clin Invest 125, 824-830. CNIO Publication.
Comino-Méndez I, Leandro-García LJ, Montoya G, Inglada-Pérez L, de Cubas AA, Currás-Freixes M, Tysoe C, Izatt L, Letón R, Gómez-Graña Á, Mancikova V, Apellániz-Ruiz M, Mannelli M, Schiavi F, Favier J, Gimenez-Roqueplo AP, Timmers HJ, Roncador G, Garcia JF, Rodríguez-Antona C, Robledo M, Cascón A. (2015). Functional and in silico assessment of MAX variants of unknown significance.. J Mol Med-Jmm 93, 1247-1255. CNIO Publication.
Negoro T, Shimizu S, Narushima M, Banham AH, Wakabayashi H, Takayanagi R, Hagiwara T, Roncador G, Osabe T, Yanai T, Kin M, Ikeda K, Endo A, Akiyama H, Nakano Y (2014). Elevated receptor for activated C kinase 1 expression is involved in intracellular Ca2+ influx and potentially associated with compromised regulatory T cell function in patients with asthma.. Clin Exp Allergy 44, 1154-1169. CNIO Publication.
Metcalf RA, Monabati A, Vyas M, Roncador G, Gualco G, Bacchi CE, Younes SF, Natkunam Y, Freud AG (2014). Myeloid cell nuclear differentiation antigen is expressed in a subset of marginal zone lymphomas and is useful in the differential diagnosis with follicular lymphoma.. Hum Pathol 45, 1730-1736. CNIO Publication.
Kempf W, Kazakov DV, Cipolat C, Kutzner H, Roncador G, Tomasini D (2014). In Response.. Am J Dermatopath 36, 103. CNIO Publication.
Patel S, Murphy D, Haralambieva E, Abdulla ZA, Wong KK, Chen H, Gould E, Roncador G, Hatton CS, Anderson AP, Banham AH, Pulford K (2014). Increased Expression of Phosphorylated FADD in Anaplastic Large Cell and Other T-Cell Lymphomas.. Biomark Insights 3, 77-84. CNIO Publication.
Martín-Sánchez E, Odqvist L, Rodríguez-Pinilla SM, Sánchez-Beato M, Roncador G, Domínguez-González B, Blanco-Aparicio C, García Collazo AM, Cantalapiedra EG, Fernández JP, Curiel del Olmo S, Pisonero H, Madureira R, Almaraz C, Mollejo M, Alves FJ, Menárguez J, González-Palacios F, Rodríguez-Peralto JL, Ortiz-Romero PL, Real FX, García JF, Bischoff JR, Piris MA (2014). PIM kinases as potential therapeutic targets in a subset of peripheral T cell lymphoma cases.. PLoS ONE 9, e1121148. CNIO Publication.
Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, Luque-Garcia JL, Fernandez-Tresguerres B, Fernandez AF, Fraga MF, Rodriguez-Justo M, Manzanares M, Sánchez-Carbayo M, García-Pedrero JM, Rodrigo JP, Malumbres M, Serrano M (2014). Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia.. Nat Communications 5, 4226. CNIO Publication.