Giovanna Roncador Head of Unit
T +34 917328000 (Ext 3130)
groncador@cnio.es

Due to their high specificity and selectivity, monoclonal antibodies (mAbs) are exquisite tools that enable researchers to address basic questions in biology; they are currently one of the most important classes of reagents used in biomedicine.

The Monoclonal Antibodies Unit provides CNIO Research Groups with an “à la carte” generation of mAbs. We are highly specialised in the production of mouse and rat monoclonal antibodies. The Unit also offers mAb characterisation and validation, mediumscale mAb production and a service of Mycoplasma testing for the cell culture facility.

Publications

• Genomic Profile and Pathologic Features of Diffuse Large B-Cell Lymphoma Subtype of Methotrexate-associated Lymphoproliferative Disorder in Rheumatoid Arthritis Patients.(2018)
  Carreras J, Yukie Kikuti Y, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Shiraiwa S, Ando K, Sato S, Suzuki Y, Miura I, Roncador G, Nakamura N.
  Am J Surg Pathol 42, 936-950.
• Overlap at the molecular and immunohistochemical levels between angioimmunoblastic T-cell lymphoma and a subgroup of peripheral T-cell lymphomas without specific morphological features.(2018)
  Manso R, González-Rincón J, Rodríguez-Justo M, Roncador G, Gómez S, Sánchez-Beato M, Piris MA, Rodríguez-Pinilla SM
  Oncotarget 9, 16124-16133.
• Nkx2-3-A Slippery Slope From Development Through Inflammation Toward Hematopoietic Malignancies.(2018)
  Vojkovics D, Kellermayer Z, Kajtár B, Roncador G, Vincze Á, Balogh P
  Biomark Insights 13, 1-6.
• Almost 2% of Spanish breast cancer families are associated to germline pathogenic mutations in the ATM gene.(2017)
  Tavera-Tapia A, Pérez-Cabornero L, Macías JA, Ceballos MI, Roncador G, de la Hoya M, Barroso A, Felipe-Ponce V, Serrano-Blanch R, Hinojo C, Miramar-Gallart MD, Urioste M, Caldés T, Santillan-Garzón S, Benitez J, Osorio A
  Breast Cancer Res Treat 161, 117-134.
• p-MAPK1 expression associated with poor prognosis in angioimmunoblastic T-cell lymphoma patients.(2017)
  Manso R, Roncador G, Montes-Moreno S, Rojo F, Pérez-Sáenz MÁ, Mollejo M, Menárguez J, Carvajal N, García-Cosio M, Llamas P, Piris MA, Rodríguez-Pinilla SM
  Br J Haematol 176, 661-664.
• Clinicopathological characteristics and genomic profile of primary sinonasal tract diffuse large B-cell lymphoma (DLBCL) reveals gain at 1q31 and RGS1 encoding protein; high RGS1 immunohistochemical expression associates with poor overall survival in DLBCL NOS.(2017)
  Carreras J, Kikuti YY, Beà S, Miyaoka M, Hiraiwa S, Ikoma H, Nagao R, Martin-Garcia D, Salaverria I, Sato A, Akifumi I, Roncador G, Garcia JF, Ando K, Campo E, Nakamura N
  Histopathology 70, 595-621.
• KLHL6 is preferentially expressed in germinal center-derived B-cell lymphomas.(2017)
  Kunder CA, Roncador G, Advani RH, Gualco G, Bacchi CE, Sabile JM, Lossos IS, Nie K, Tibshirani RJ, Green MR, Alizadeh AA, Natkunam Y
  Am J Clin Pathol 148, 465-476.
• Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers.(2016)
  Pérez-Guijarro E, Karras P, Cifdaloz M, Martínez-Herranz R, Cañón E, Graña O, Horcajada-Reales C, Alonso-Curbelo D, Calvo TG, Gómez-López G, Bellora N, Riveiro-Falkenbach E, Ortiz-Romero PL, Rodríguez-Peralto JL, Maestre L, Roncador G, de Agustín Asensio JC, Goding CR, Eyras E, Megías D, Méndez R, Soengas MS
  Nat Communications 7, 13418-.
• FOXP2-positive diffuse large B-cell lymphomas exhibit a poor response to R-CHOP therapy and distinct biological signatures.(2016)
  Wong KK, Gascoyne DM, Soilleux EJ, Lyne L, Spearman H, Roncador G, Pedersen LM, Møller MB, Green TM, Banham AH
  Oncotarget 7, 52940-52956.
• The European antibody network’s practical guide to finding and validating suitable antibodies for research.(2016)
  Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS, Šerbec VC, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa JI, Torensma R, Turley H, Pulford K, Banham AH
  MABS 8, 27-36.
• NOTCH pathway inactivation promotes bladder cancer progression.(2015)
  Maraver A, Fernandez-Marcos PJ, Cash TP, Mendez-Pertuz M, Dueñas M, Maietta P, Martinelli P, Muñoz-Martin M, Martínez-Fernández M, Cañamero M, Roncador G, Martinez-Torrecuadrada JL, Grivas D, de la Pompa JL, Valencia A, Paramio JM, Real FX, Serrano M
  J Clin Invest 125, 824-830.
• Whole-Exome Sequencing Identifies MDH2 as a New Familial Paraganglioma Gene.(2015)
  Cascón A, Comino-Méndez I, Currás-Freixes M, de Cubas AA, Contreras L, Richter S, Peitzsch M, Mancikova V, Inglada-Pérez L, Pérez-Barrios A, Calatayud M, Azriel S, Villar-Vicente R, Aller J, Setién F, Moran S, Garcia JF, Río-Machín A, Letón R, Gómez-Graña Á, Apellániz-Ruiz M, Roncador G, Esteller M, Rodríguez-Antona C, Satrústegui J, Eisenhofer G, Urioste M, Robledo M
  J Natl Cancer I 107, djv053-.
• Functional and in silico assessment of MAX variants of unknown significance.(2015)
  Comino-Méndez I, Leandro-García LJ, Montoya G, Inglada-Pérez L, de Cubas AA, Currás-Freixes M, Tysoe C, Izatt L, Letón R, Gómez-Graña Á, Mancikova V, Apellániz-Ruiz M, Mannelli M, Schiavi F, Favier J, Gimenez-Roqueplo AP, Timmers HJ, Roncador G, Garcia JF, Rodríguez-Antona C, Robledo M, Cascón A.
  J Mol Med-Jmm 93, 1247-1255.
• CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma.(2015)
  Martín-Moreno AM, Roncador G, Maestre L, Mata E, Jiménez S, Martínez-Torrecuadrada JL, Reyes-García AI, Rubio C, Tomás JF, Estévez M, Pulford K, Piris MA, García JF.
  PLoS ONE 10, e0125203-.
• PIM kinases as potential therapeutic targets in a subset of peripheral T cell lymphoma cases.(2014)
  Martín-Sánchez E, Odqvist L, Rodríguez-Pinilla SM, Sánchez-Beato M, Roncador G, Domínguez-González B, Blanco-Aparicio C, García Collazo AM, Cantalapiedra EG, Fernández JP, Curiel del Olmo S, Pisonero H, Madureira R, Almaraz C, Mollejo M, Alves FJ, Menárguez J, González-Palacios F, Rodríguez-Peralto JL, Ortiz-Romero PL, Real FX, García JF, Bischoff JR, Piris MA
  PLoS ONE 9, e1121148-.
• Lineage-restricted function of the pluripotency factor NANOG in stratified epithelia.(2014)
  Piazzolla D, Palla AR, Pantoja C, Cañamero M, de Castro IP, Ortega S, Gómez-López G, Dominguez O, Megías D, Roncador G, Luque-Garcia JL, Fernandez-Tresguerres B, Fernandez AF, Fraga MF, Rodriguez-Justo M, Manzanares M, Sánchez-Carbayo M, García-Pedrero JM, Rodrigo JP, Malumbres M, Serrano M
  Nat Communications 5, 4226-.
• Elevated receptor for activated C kinase 1 expression is involved in intracellular Ca2+ influx and potentially associated with compromised regulatory T cell function in patients with asthma.(2014)
  Negoro T, Shimizu S, Narushima M, Banham AH, Wakabayashi H, Takayanagi R, Hagiwara T, Roncador G, Osabe T, Yanai T, Kin M, Ikeda K, Endo A, Akiyama H, Nakano Y
  Clin Exp Allergy 44, 1154-1169.
• Myeloid cell nuclear differentiation antigen is expressed in a subset of marginal zone lymphomas and is useful in the differential diagnosis with follicular lymphoma.(2014)
  Metcalf RA, Monabati A, Vyas M, Roncador G, Gualco G, Bacchi CE, Younes SF, Natkunam Y, Freud AG
  Hum Pathol 45, 1730-1736.
• In Response.(2014)
  Kempf W, Kazakov DV, Cipolat C, Kutzner H, Roncador G, Tomasini D
  Am J Dermatopath 36, 103-.
• Increased Expression of Phosphorylated FADD in Anaplastic Large Cell and Other T-Cell Lymphomas.(2014)
  Patel S, Murphy D, Haralambieva E, Abdulla ZA, Wong KK, Chen H, Gould E, Roncador G, Hatton CS, Anderson AP, Banham AH, Pulford K
  Biomark Insights 3, 77-84.
• SPIB, a novel immunohistochemical marker for human blastic plasmacytoid dendritic cell neoplasms: characterization of its expression in major hematolymphoid neoplasms.(2013)
  Montes-Moreno S, Ramos-Medina R, Martínez-López A, Barrionuevo Cornejo C, Parra Cubillos A, Quintana-Truyenque S, Rodriguez Pinilla SM, Pajares R, Sanchez-Verde L, Martinez-Torrecuadrada J, Roncador G, Piris MA
  Blood 121, 643-647.
• BCL7A protein expression in normal and malignant lymphoid tissues.(2013)
  Ramos-Medina R, Montes-Moreno S, Maestre L, Cañamero M, Rodríguez-Pinilla M, Martínez-Torrecuadrada JL, Piris MA, Majid A, Dyer MJ, Pulford K, Roncador G
  Br J Haematol 160, 106-109.
• MAP17 and SGLT1 protein expression levels as prognostic markers for cervical tumor patient survival.(2013)
  Perez M, Praena-Fernandez JM, Felipe-Abrio B, Lopez-Garcia MA, Lucena-Cacace A, Garcia A, Lleonart M, Roncador G, Marin JJ, Carnero A
  PLoS ONE 8, e56169-.