Monoclonal Antibodies Core Unit

Giovanna Roncador Head of Unit
T +34 917328000 (Ext 3130)
groncador@cnio.es

Technicians

Álvaro García
Scherezade Jiménez
Lorena Maestre
Ana Isabel Reyes

Since the discovery of hybridoma technology by Caesar Milstein and Georges Köhler in 1975, monoclonal antibodies (mAbs) have become one of the most relevant methodological advances in biomedicine.

MAbs have provided researchers with the ability to study biological processes reliably and with unprecedented accuracy, improving our knowledge about the processes involved in tumour generation and development. Beyond their applications in the laboratory as research tools, mAbs are also used in the area of diagnostics, and serve as therapeutic agents in the treatment of cancer.

The Monoclonal Antibodies Unit provides CNIO Research Groups with à la carte generation of mAbs using hybridoma technology. We are highly specialised in the production of mouse and rat monoclonal antibodies. The Unit also offers mAb characterisation and validation, medium-scale mAb production, as well as a Mycoplasma testing service for the cell culture facility.

Publications

Yamamoto Y, Carreras J, Shimizu T, Kakizaki M, Kikuti YY, Roncador G, Nakamura N, Kotani A (2022). Anti-HBV drug entecavir ameliorates DSS-induced colitis through PD-L1 induction. Pharmacol Res 179, 105918. CNIO Publication.
Peña-Cardelles JF, Pozo-Kreilinger JJ, Roncador G, Esteban-Hernández J, Moro-Rodríguez JE, Sastre-Perona A, Castelo-Fernández B, Cebrián-Carretero JL. (2022). Prognosis Value of Immunoregulatory Molecules in Oral Cancer Microenvironment: An Immunohistochemical Study. Biomedicines 10, 710. CNIO Publication.
Carreras J, Hiraiwa S, Kikuti YY, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Roncador G, Garcia JF, Ando K, Hamoudi R, Nakamura N (2021). Artificial neural networks predicted the overall survival and molecular subtypes of diffuse large B-cell lymphoma using a pancancer immune-oncology panel.. Cancers 13, 6384. CNIO Publication.
Carreras J, Kikuti YY, Hiraiwa S, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Itoh J, Roncador G, Martinez A, Colomo L, Hamoudi R, Ando K, Nakamura N. (2021). High PTX3 expression is associated with a poor prognosis in diffuse large B-cell lymphoma. Cancer Sci 113, 334-348. CNIO Publication.
Monteagudo M, Martínez P, Leandro-García LJ, Martínez-Montes ÁM, Calsina B, Pulgarín-Alfaro M, Díaz-Talavera A, Mellid S, Letón R, Gil E, Pérez-Martínez M, Megías D, Torres-Ruiz R, Rodriguez-Perales S, González P, Caleiras E, Jiménez-Villa S, Roncador G, Álvarez-Escolá C, Regojo RM, Calatayud M, Guadalix S, Currás-Freixes M, Rapizzi E, Canu L, Nölting S, Remde H, Fassnacht M, Bechmann N, Eisenhofer G, Mannelli M, Beuschlein F, Quinkler M, Rodríguez-Antona C, Cascón A, Blasco MA, Montero-Conde C, Robledo M (2021). Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma. Cancers 13, 4758. CNIO Publication.
Voskuil JLA, Bandrowski A, Begley CG, Bradbury ARM, Chalmers AD, Gomes AV, Hardcastle T, Lund-Johansen F, Plückthun A, Roncador G, Solache A, Taussig MJ, Trimmer JS, Williams C, Goodman (2020). The Antibody Society’s antibody validation webinar series.. MABS 12, 1794421. CNIO Publication.
Maestre L, García-García JF, Jiménez S, Reyes-García AI, García-González Á, Montes-Moreno S, Arribas AJ, González-García P, Caleiras E, Banham AH, Piris MÁ, Roncador G. (2020). High-mobility group box (TOX) antibody a useful tool for the identification of B and T cell subpopulations. PLoS One 15, e0229743. CNIO Publication. Open Access
Tsuda S, Carreras J, Kikuti Y, Nakae H, Dekiden-Monma M, Imai J, Tsuruya K, Nakamura J, Tsukune Y, Uchida T, Matsushima M, Roncador G, Suzuki T, Nakamura N, Mine T (2019). Prediction of steroid demand in the treatment of patients with ulcerative colitis by immunohistochemical analysis of the mucosal microenvironment and immune checkpoint: role of macrophages and regulatory markers in disease severity. Pathol Int 69, 260-271. CNIO Publication.
Carreras J, Lopez-Guillermo A, Kikuti YY, Itoh J, Masashi M, Ikoma H, Tomita S, Hiraiwa S, Hamoudi R, Rosenwald A, Leich E, Martinez A, Roncador G, Villamor N, Colomo L, Perez P, Tsuji NM, Campo E, Nakamura N (2019). High TNFRSF14 and low BTLA are associated with poor prognosis in Follicular Lymphoma and in Diffuse Large B-cell Lymphoma transformation. J Clin Exp Hematop 59, 1-16. CNIO Publication.
Carreras J, Yukie Kikuti Y, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Shiraiwa S, Ando K, Sato S, Suzuki Y, Miura I, Roncador G, Nakamura N (2018). Genomic Profile and Pathologic Features of Diffuse Large B-Cell Lymphoma Subtype of Methotrexate-associated Lymphoproliferative Disorder in Rheumatoid Arthritis Patients. Am J Surg Pathol 42, 936-950. CNIO Publication.
Manso R, González-Rincón J, Rodríguez-Justo M, Roncador G, Gómez S, Sánchez-Beato M, Piris MA, Rodríguez-Pinilla SM (2018). Overlap at the molecular and immunohistochemical levels between angioimmunoblastic T-cell lymphoma and a subgroup of peripheral T-cell lymphomas without specific morphological features. Oncotarget 9, 16124-16133. CNIO Publication. Open Access
Vojkovics D, Kellermayer Z, Kajtár B, Roncador G, Vincze Á, Balogh P (2018). Nkx2-3-A Slippery Slope From Development Through Inflammation Toward Hematopoietic Malignancies. Biomark Insights 13, 1-6. CNIO Publication. Open Access
Manso R, Roncador G, Montes-Moreno S, Rojo F, Pérez-Sáenz MÁ, Mollejo M, Menárguez J, Carvajal N, García-Cosio M, Llamas P, Piris MA, Rodríguez-Pinilla SM (2017). p-MAPK1 expression associated with poor prognosis in angioimmunoblastic T-cell lymphoma patients. Br J Haematol 176, 661-664. CNIO Publication.
Carreras J, Kikuti YY, Beà S, Miyaoka M, Hiraiwa S, Ikoma H, Nagao R, Martin-Garcia D, Salaverria I, Sato A, Akifumi I, Roncador G, Garcia JF, Ando K, Campo E, Nakamura N (2017). Clinicopathological characteristics and genomic profile of primary sinonasal tract diffuse large B-cell lymphoma (DLBCL) reveals gain at 1q31 and RGS1 encoding protein; high RGS1 immunohistochemical expression associates with poor overall survival in DLBCL NOS. Histopathology 70, 595-621. CNIO Publication.
Tavera-Tapia A, Pérez-Cabornero L, Macías JA, Ceballos MI, Roncador G, de la Hoya M, Barroso A, Felipe-Ponce V, Serrano-Blanch R, Hinojo C, Miramar-Gallart MD, Urioste M, Caldés T, Santillan-Garzón S, Benitez J, Osorio A (2017). Almost 2% of Spanish breast cancer families are associated to germline pathogenic mutations in the ATM gene. Breast Cancer Res Treat 161, 117-134. CNIO Publication.
Kunder CA, Roncador G, Advani RH, Gualco G, Bacchi CE, Sabile JM, Lossos IS, Nie K, Tibshirani RJ, Green MR, Alizadeh AA, Natkunam Y (2017). KLHL6 is preferentially expressed in germinal center-derived B-cell lymphomas. Am J Clin Pathol 148, 465-476. CNIO Publication.