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Assays Panel

Unit Leader: Luís Lombardía

Assays Panel
Molecular alteration  BIOMARKER Technique
Gene expression t(9;22)  or BCR-ABL fusion gene qRT-PCR
t(14;18)  or BCL2-IGH fusion gene qRT-PCR
t(8;21) or AML1- ETO fusion gene qRT-PCR
 t(15;17) or PML-RARA fusion gene qRT-PCR
Inv16  or MYH11-CBFB fusion gene qRT-PCR
Whole genome gene expression DNA Arrays
Mutations JAK2 qRT-PCR or direct sequencing
BCR-ABL (fusion gene) Direct sequencing
EGFR Direct sequencing
GATA1 Direct sequencing
KRAS Direct sequencing
BRAF Direct sequencing
FLT3  PCR and capilary electrophoresis 
KIT Direct sequencing
PDGFRA Direct sequencing
NPM1 Direct sequencing
PI3KCA Direct sequencing
NRAS Direct sequencing
MPL Direct sequencing
PTEN Direct sequencing 
AKT1 Direct sequencing
Rearrangements IGH PCR and capilary electrophoresis 
IGK PCR and capilary electrophoresis 
TCRγ PCR and capilary electrophoresis 
TCRβ PCR and capilary electrophoresis 
Microsatellite instability PCR and capilary electrophoresis 
Copy number variation cMET amplification qRT-PCR