National Health System Support Services
Molecular Diagnostics
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Inmunohistoquímica/ Programa de Biotecnología
- Contact
- Lydia Sánchez
+(34) 917 328 000
The immunohistochemistry section has a catalog of about 260 antibodies and probes to test the expression of specific markers useful in the diagnosis and prognosis of cancer.
Tests
- Histology
Indication
Technique used for microscopic study of tissues.
Test description
Tissue sections cut and stained with different histological stains such as: Hematoxylin & Eosin, Alcian Blue, Congo Red, Giemsa, Gram, Masson’s Trichrome, Grocott´s Methenamine silver, PAS, Reticulin, Thioflavin, Warthin-Starry or Ziehl-Neelsen.
- Immunohistochemistry
Indication
Technique used to determine the expression of certain proteins in a tissue.
Test description
Tissue sections cut and protein expression detected by specific antibodies. There are two different methods available for signal detection: the Labeled Streptavidin Biotin (LSAB™) method, which is more sensitive than conventional methods; and the Envision® method based on dextran polymer technology, which is more sensitive and minimizes non-specific background staining.
- In situ hybridization (ISH)
Indication
In situ hybridization is used to localize DNA or RNA specific sequences in cells of tissue samples.
Test description
Detection of Herpesvirus 8 (HHV-8), Epstein-Barr virus (EBV) and the kappa and lambda chains of human immunoglobulins with specific labeled probes. A probe is a known sequence of nucleotides that is complementary to the sequence of interest.
Specimen Requirements
Timely delivery/ notification is essential. Please call for all incoming specimens (912 246 916)
If you need additional information, please contact us +34 912 246 917.
- Tissue Requirements
- Tissue requirements:
- Please send 10 % buffered formalin fixed, fresh or frozen tissue containing representative tumor. When possible, send 1 cm cubic of tissue.dentro de las 6 horas siguientes a su extracción.
- For paraffin blocks, please send the entire block containing representative tumor.
- PFor tissue sections, please send unstained 4-micron formalin fixed paraffin-embedded tissue sections on electrostatically charged slides containing representative tumor.
- Place fresh specimens in sterile saline or in a tube containing culture media, or PBS and Gentamycin. The tissue should be delivered within 6 hours of removal.
- Same day shipments of fresh solid tumor specimens should be kept at room temperature until shipped.
- Overnight shipments of fresh tissue specimens should be refrigerated. DO NOT FREEZE!
- Frozen tissue should be kept frozen at least at –70ºC and ship on dry ice.
- Formalin fixed and paraffin-embedded tissue should be shipped at room temperature.
- Tissue requirements:
